Manganese in PDB 2am3: Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose

All present enzymatic activity of Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose:
2.4.1.101;

The structure of Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose, PDB code: 2am3 was solved by J.M.Rini, R.D.Gordon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.10 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.628, 82.492, 102.250, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 22.6

The binding sites of Manganese atom in the Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose (pdb code 2am3). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose, PDB code: 2am3:

Manganese binding site 1 out of 1 in the Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with Udp-Glucose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn448 b:13.2 occ:1.00 OD2 A:ASP213 2.2 12.7 1.0 O2A A:UPG449 2.2 15.9 1.0 O A:HOH554 2.2 17.3 1.0 O A:HOH489 2.2 14.5 1.0 O2B A:UPG449 2.2 21.8 1.0 O A:HOH533 2.3 14.8 1.0 CG A:ASP213 3.1 12.3 1.0 PA A:UPG449 3.4 14.4 1.0 OD1 A:ASP213 3.4 13.3 1.0 PB A:UPG449 3.5 27.6 1.0 O3A A:UPG449 3.7 14.1 1.0 NH1 A:ARG117 4.0 16.9 1.0 NH2 A:ARG117 4.0 9.4 1.0 O A:GLY317 4.3 28.7 1.0 O1A A:UPG449 4.3 15.0 1.0 O2' A:UPG449 4.3 33.8 1.0 O1B A:UPG449 4.3 31.4 1.0 O5C A:UPG449 4.4 9.7 1.0 OG1 A:THR315 4.4 14.9 1.0 OE1 A:GLU211 4.4 19.4 1.0 CB A:ASP213 4.5 11.0 1.0 C5C A:UPG449 4.5 11.8 1.0 CZ A:ARG117 4.5 13.7 1.0 O3B A:UPG449 4.7 29.9 1.0 CA A:GLY317 4.9 25.7 1.0

R.D.Gordon, P.Sivarajah, M.Satkunarajah, D.Ma, C.A.Tarling, D.Vizitiu, S.G.Withers, J.M.Rini. X-Ray Crystal Structures of Rabbit N-Acetylglucosaminyltransferase I (Gnt I) in Complex with Donor Substrate Analogues. J.Mol.Biol. V. 360 67 2006.
ISSN: ISSN 0022-2836
PubMed: 16769084
DOI: 10.1016/J.JMB.2006.04.058