Manganese in PDB 1gx0: Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex

All present enzymatic activity of Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex:
2.4.1.151;

The structure of Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex, PDB code: 1gx0 was solved by E.Boix, Y.Zhang, G.J.Swaminathan, K.Brew, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.19 / 1.8
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.059, 94.277, 94.364, 90.00, 99.09, 90.00
R / Rfree (%)	18.9 / 21.1

The binding sites of Manganese atom in the Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex (pdb code 1gx0). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex, PDB code: 1gx0:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1370 b:19.6 occ:1.00 O1B A:UDP1369 2.1 20.1 1.0 OD2 A:ASP225 2.2 14.9 1.0 OD1 A:ASP227 2.2 20.1 1.0 O A:HOH2250 2.2 16.9 1.0 O1A A:UDP1369 2.3 16.4 1.0 OD2 A:ASP227 2.4 19.8 1.0 CG A:ASP227 2.6 20.2 1.0 CG A:ASP225 3.2 16.3 1.0 PB A:UDP1369 3.3 19.9 1.0 O3A A:UDP1369 3.4 20.4 1.0 PA A:UDP1369 3.4 20.6 1.0 CB A:ASP225 3.6 16.1 1.0 NZ A:LYS359 3.7 18.2 1.0 O3B A:UDP1369 3.8 21.0 1.0 O3' A:UDP1369 3.8 17.8 1.0 O A:HOH2248 3.9 24.9 1.0 CB A:ASP227 4.1 19.5 1.0 O A:HOH2233 4.2 21.9 1.0 O A:HOH2132 4.3 29.6 1.0 OD1 A:ASP225 4.3 17.8 1.0 O5' A:UDP1369 4.3 19.7 1.0 C5' A:UDP1369 4.4 17.3 1.0 O A:HOH2229 4.5 20.8 1.0 O A:HOH2249 4.5 17.9 1.0 O2B A:UDP1369 4.6 20.7 1.0 O A:HOH2133 4.6 18.3 1.0 C3' A:UDP1369 4.6 19.3 1.0 O2A A:UDP1369 4.7 21.2 1.0 O A:ASP227 4.8 17.9 1.0 CB A:ALA282 4.8 17.1 1.0 C4' A:UDP1369 4.9 18.3 1.0 N A:ASP227 4.9 18.5 1.0 CA A:ASP227 5.0 18.8 1.0 CA A:ASP225 5.0 16.9 1.0

Manganese binding site 2 out of 2 in the Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Alpha-,1,3 Galactosyltransferase - Beta-D-Galactose Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1370 b:20.4 occ:1.00 O1B B:UDP1369 2.1 18.5 1.0 O B:HOH2245 2.2 16.8 1.0 OD2 B:ASP225 2.2 16.5 1.0 OD1 B:ASP227 2.2 20.9 1.0 O1A B:UDP1369 2.2 15.0 1.0 OD2 B:ASP227 2.5 16.9 1.0 CG B:ASP227 2.7 19.6 1.0 CG B:ASP225 3.2 18.9 1.0 PB B:UDP1369 3.3 19.7 1.0 PA B:UDP1369 3.4 19.2 1.0 O3A B:UDP1369 3.4 19.6 1.0 CB B:ASP225 3.6 15.1 1.0 O3B B:UDP1369 3.7 20.2 1.0 NZ B:LYS359 3.7 19.2 1.0 O3' B:UDP1369 3.8 18.4 1.0 O B:HOH2241 4.1 23.9 1.0 CB B:ASP227 4.2 17.3 1.0 O B:HOH2228 4.2 20.3 1.0 O5' B:UDP1369 4.3 19.1 1.0 OD1 B:ASP225 4.3 18.7 1.0 O6 B:GAL1371 4.5 52.7 1.0 C5' B:UDP1369 4.5 17.4 1.0 O B:HOH2126 4.5 19.6 1.0 O B:HOH2128 4.5 19.3 1.0 O2B B:UDP1369 4.6 20.6 1.0 O2A B:UDP1369 4.6 19.2 1.0 O B:HOH2225 4.6 21.3 1.0 C3' B:UDP1369 4.6 17.5 1.0 CB B:ALA282 4.7 14.8 1.0 O B:ASP227 4.8 18.1 1.0 C4' B:UDP1369 4.9 17.1 1.0 CA B:ASP225 5.0 17.1 1.0 N B:ASP227 5.0 16.3 1.0 CA B:ASP227 5.0 17.5 1.0

E.Boix, Y.Zhang, G.J.Swaminathan, K.Brew, K.R.Acharya. Structural Basis of Ordered Binding of Donor and Acceptor Substrates to the Retaining Glycosyltransferase, Alpha -1,3 Galactosyltransferase J.Biol.Chem. V. 277 28310 2002.
ISSN: ISSN 0021-9258
PubMed: 12011052
DOI: 10.1074/JBC.M202631200