Manganese in PDB 2ilz: Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+

All present enzymatic activity of Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+:
2.7.7.48;

The structure of Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+, PDB code: 2ilz was solved by A.A.Thompson, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.30 / 2.50
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	128.159, 128.159, 112.610, 90.00, 90.00, 120.00
R / Rfree (%)	23 / 25.5

The structure of Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+ also contains other interesting chemical elements:

Arsenic	(As)	4 atoms
Sodium	(Na)	2 atoms

The binding sites of Manganese atom in the Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+ (pdb code 2ilz). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+, PDB code: 2ilz:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2001 b:99.4 occ:1.00 OD2 A:ASP328 2.8 43.2 1.0 OD1 A:ASP328 2.9 44.8 1.0 CG A:ASP328 3.1 41.5 1.0 O A:HOH653 3.2 40.8 1.0 OD2 A:ASP329 3.8 45.1 1.0 CB A:ASP329 3.9 39.6 1.0 N A:ASP329 4.0 38.3 1.0 CG A:ASP329 4.0 42.3 1.0 CA A:ASP329 4.1 36.9 1.0 MN A:MN2002 4.2 0.5 1.0 C A:ASP328 4.5 39.5 1.0 CB A:ASP328 4.5 39.2 1.0 OD1 A:ASP329 4.8 46.2 1.0 O A:HOH542 4.9 50.1 1.0 O A:ASP328 5.0 41.9 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Poliovirus Polymerase Complexed with Gtp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2002 b:0.5 occ:1.00 O2B A:GTP1001 1.8 92.8 1.0 PB A:GTP1001 2.8 90.9 1.0 O A:TYR234 2.9 41.2 1.0 O3B A:GTP1001 3.1 95.6 1.0 O A:HOH542 3.4 50.1 1.0 O1B A:GTP1001 3.5 92.4 1.0 OD2 A:ASP328 3.8 43.2 1.0 OD2 A:ASP233 4.1 63.8 1.0 C A:TYR234 4.1 39.6 1.0 MN A:MN2001 4.2 99.4 1.0 O3A A:GTP1001 4.3 88.9 1.0 O A:HOH631 4.3 51.6 1.0 O2G A:GTP1001 4.3 98.5 1.0 PG A:GTP1001 4.4 99.5 1.0 CB A:ASP233 4.4 56.3 1.0 N A:TYR234 4.7 44.5 1.0 O A:HOH653 4.7 40.8 1.0 CG A:ASP233 4.7 60.4 1.0

A.A.Thompson, R.A.Albertini, O.B.Peersen. Stabilization of Poliovirus Polymerase By Ntp Binding and Fingers-Thumb Interactions. J.Mol.Biol. V. 366 1459 2007.
ISSN: ISSN 0022-2836
PubMed: 17223130
DOI: 10.1016/J.JMB.2006.11.070