Manganese in PDB 7lpy: Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases

The structure of Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases, PDB code: 7lpy was solved by R.Thapar, A.S.Andrew, J.A.Tainer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.32 / 1.85
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	58.555, 74.639, 96.709, 90, 90, 90
R / Rfree (%)	17.9 / 20.5

The binding sites of Manganese atom in the Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases (pdb code 7lpy). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases, PDB code: 7lpy:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn502 b:39.0 occ:1.00 O2P A:AMP501 1.9 39.8 1.0 O A:HOH608 1.9 48.9 1.0 OD2 A:ASP312 1.9 38.9 1.0 OD2 A:ASP150 2.1 37.2 1.0 OE2 A:GLU152 2.1 42.5 1.0 CG A:ASP312 3.1 43.7 1.0 CD A:GLU152 3.2 46.2 1.0 CG A:ASP150 3.2 40.7 1.0 P A:AMP501 3.3 42.1 1.0 OE1 A:GLU152 3.6 42.1 1.0 OD1 A:ASP150 3.6 38.8 1.0 MN A:MN503 3.6 45.7 1.0 CB A:ASP312 3.7 36.2 1.0 O3P A:AMP501 3.8 40.9 1.0 O5' A:AMP501 3.9 38.2 1.0 O A:PHE151 4.0 38.2 1.0 O A:HOH685 4.1 49.8 1.0 OD1 A:ASP312 4.1 40.2 1.0 CA A:HIS307 4.2 46.0 1.0 O A:SER308 4.3 42.4 1.0 O A:HOH639 4.3 37.7 1.0 C A:HIS307 4.4 48.6 1.0 CG A:GLU152 4.4 38.0 1.0 O1P A:AMP501 4.4 38.6 1.0 CA A:GLY309 4.4 34.5 1.0 CB A:ASP150 4.5 38.8 1.0 C5' A:AMP501 4.5 36.4 1.0 C A:SER308 4.5 41.9 1.0 N A:GLY309 4.6 40.3 1.0 O A:HIS307 4.7 47.7 1.0 N A:SER308 4.9 46.1 1.0 C A:PHE151 4.9 38.6 1.0 N A:HIS307 4.9 46.0 1.0

Manganese binding site 2 out of 4 in the Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn503 b:45.7 occ:1.00 O A:HOH639 2.2 37.7 1.0 OD1 A:ASP150 2.2 38.8 1.0 O A:HOH603 2.3 36.9 1.0 O2P A:AMP501 2.3 39.8 1.0 O1P A:AMP501 2.3 38.6 1.0 O A:HOH685 2.4 49.8 1.0 P A:AMP501 2.8 42.1 1.0 CG A:ASP150 3.1 40.7 1.0 OD2 A:ASP150 3.4 37.2 1.0 MN A:MN502 3.6 39.0 1.0 O5' A:AMP501 3.9 38.2 1.0 O3P A:AMP501 3.9 40.9 1.0 O A:HOH629 4.0 35.7 1.0 C5' A:AMP501 4.1 36.4 1.0 OD2 A:ASP249 4.1 41.6 1.0 OD1 A:ASP249 4.3 39.5 1.0 O A:PHE151 4.4 38.2 1.0 CB A:ASP150 4.4 38.8 1.0 O A:CYS245 4.5 44.5 1.0 CG A:ASP249 4.6 39.7 1.0 O A:HOH608 4.7 48.9 1.0 OD2 A:ASP312 4.7 38.9 1.0 CA A:ASP150 4.8 37.6 1.0

Manganese binding site 3 out of 4 in the Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn502 b:45.7 occ:1.00 O2P B:AMP501 1.9 45.5 1.0 OD2 B:ASP312 2.0 42.2 1.0 OD2 B:ASP150 2.0 40.9 1.0 OE2 B:GLU152 2.1 44.0 1.0 O B:HOH610 2.3 54.5 1.0 CG B:ASP312 3.1 43.8 1.0 CG B:ASP150 3.1 42.3 1.0 CD B:GLU152 3.1 45.0 1.0 P B:AMP501 3.3 46.9 1.0 OD1 B:ASP150 3.5 39.5 1.0 OE1 B:GLU152 3.6 47.1 1.0 MN B:MN503 3.7 50.9 1.0 CB B:ASP312 3.7 40.4 1.0 O1P B:AMP501 3.9 46.1 1.0 O B:PHE151 3.9 39.1 1.0 O5' B:AMP501 4.0 44.4 1.0 OD1 B:ASP312 4.1 38.3 1.0 O B:SER308 4.2 46.0 1.0 CA B:HIS307 4.2 55.2 1.0 O B:HOH648 4.3 44.3 1.0 C B:HIS307 4.4 55.1 1.0 O B:HOH606 4.4 38.4 1.0 O3P B:AMP501 4.4 54.9 1.0 CB B:ASP150 4.4 40.8 1.0 CG B:GLU152 4.4 36.8 1.0 CA B:GLY309 4.4 34.9 1.0 C5' B:AMP501 4.5 49.1 1.0 C B:SER308 4.5 44.9 1.0 N B:GLY309 4.6 42.0 1.0 O B:HIS307 4.6 62.0 1.0 C B:PHE151 4.9 45.7 1.0 N B:SER308 4.9 53.9 1.0 O B:ASP150 4.9 44.1 1.0 N B:HIS307 5.0 51.9 1.0

Manganese binding site 4 out of 4 in the Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3-Prime - 5-Prime Exoribonucleases within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn503 b:50.9 occ:1.00 OD1 B:ASP150 2.1 39.5 1.0 O B:HOH608 2.1 47.0 1.0 O B:HOH606 2.1 38.4 1.0 O3P B:AMP501 2.2 54.9 1.0 O2P B:AMP501 2.3 45.5 1.0 O B:HOH648 2.4 44.3 1.0 P B:AMP501 2.7 46.9 1.0 CG B:ASP150 3.1 42.3 1.0 OD2 B:ASP150 3.4 40.9 1.0 MN B:MN502 3.7 45.7 1.0 O B:HOH615 3.8 43.3 1.0 O5' B:AMP501 3.9 44.4 1.0 O1P B:AMP501 3.9 46.1 1.0 C5' B:AMP501 4.0 49.1 1.0 OD2 B:ASP249 4.0 53.4 1.0 OD1 B:ASP249 4.1 44.7 1.0 O B:PHE151 4.2 39.1 1.0 CB B:ASP150 4.4 40.8 1.0 O2 B:GOL504 4.4 69.4 1.0 O3 B:GOL504 4.4 72.7 1.0 CG B:ASP249 4.5 51.8 1.0 C3 B:GOL504 4.5 71.7 1.0 O B:CYS245 4.6 50.2 1.0 OD2 B:ASP312 4.7 42.2 1.0 CA B:ASP150 4.8 40.5 1.0 O B:HOH610 4.9 54.5 1.0

R.Thapar, A.S.Arvai, J.A.Tainer. Crystal Structures and Ribonuclease Activity of the Flavivirus Host Factor ERI3 That Is Involved in Viral Rna Synthesis Define the Eri Subfamily of Structure-Specific 3' - 5' Exoribonucleases To Be Published.