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Manganese in PDB 4m4k: Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose

Enzymatic activity of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose

All present enzymatic activity of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose:
2.4.1.133;

Protein crystallography data

The structure of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose, PDB code: 4m4k was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 36.98 / 2.20
Space group P 43 21 2
Cell size a, b, c (Å), α, β, γ (°) 82.680, 82.680, 131.486, 90.00, 90.00, 90.00
R / Rfree (%) 18.7 / 21.3

Other elements in 4m4k:

The structure of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose also contains other interesting chemical elements:

Chlorine (Cl) 2 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose (pdb code 4m4k). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose, PDB code: 4m4k:

Manganese binding site 1 out of 1 in 4m4k

Go back to Manganese Binding Sites List in 4m4k
Manganese binding site 1 out of 1 in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn401

b:39.8
occ:1.00
 O1B A:GDU402 2.1 40.0 1.0
OD2 A:ASP147 2.2 35.0 1.0
O1A A:GDU402 2.2 38.7 1.0
NE2 A:HIS241 2.2 40.8 1.0
ND1 A:HIS243 2.3 40.2 1.0
O A:HOH619 2.4 35.7 1.0
CG A:ASP147 3.1 39.7 1.0
CE1 A:HIS241 3.1 41.1 1.0
CE1 A:HIS243 3.2 41.0 1.0
CD2 A:HIS241 3.2 41.7 1.0
OD1 A:ASP147 3.3 38.2 1.0
CG A:HIS243 3.4 43.5 1.0
PB A:GDU402 3.4 36.9 1.0
PA A:GDU402 3.5 40.8 1.0
CB A:HIS243 3.7 42.1 1.0
O3A A:GDU402 3.8 42.0 1.0
O A:HOH527 4.0 41.3 1.0
O3B A:GDU402 4.0 43.4 1.0
O3D A:GDU402 4.0 36.5 1.0
NH2 A:ARG250 4.1 42.6 1.0
O A:HOH502 4.2 37.8 1.0
ND1 A:HIS241 4.3 44.0 1.0
O2A A:GDU402 4.3 43.8 1.0
CG A:HIS241 4.3 42.4 1.0
NE2 A:HIS243 4.4 42.9 1.0
CB A:ASP147 4.4 39.0 1.0
CD2 A:HIS243 4.5 42.5 1.0
O2B A:GDU402 4.6 35.9 1.0
O5D A:GDU402 4.7 41.8 1.0
O A:HOH528 4.8 43.7 1.0
C3D A:GDU402 4.8 39.9 1.0
C5D A:GDU402 4.8 36.8 1.0
OD2 A:ASP145 4.8 39.2 1.0

Reference:

Y.Tsutsui, B.Ramakrishnan, P.K.Qasba. Crystal Structures of Beta-1,4-Galactosyltransferase 7 Enzyme Reveal Conformational Changes and Substrate Binding. J.Biol.Chem. V. 288 31963 2013.
ISSN: ISSN 0021-9258
PubMed: 24052259
DOI: 10.1074/JBC.M113.509984
Page generated: Sat Aug 16 14:37:36 2025

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