Manganese in PDB 2faf: The Structure of Chicken Mitochondrial Pepck.

All present enzymatic activity of The Structure of Chicken Mitochondrial Pepck.:
4.1.1.32;

The structure of The Structure of Chicken Mitochondrial Pepck., PDB code: 2faf was solved by T.Holyoak, S.M.Sullivan, T.Nowak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.08 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	110.807, 47.737, 127.205, 90.00, 111.31, 90.00
R / Rfree (%)	16.1 / 19.1

The binding sites of Manganese atom in the The Structure of Chicken Mitochondrial Pepck. (pdb code 2faf). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the The Structure of Chicken Mitochondrial Pepck., PDB code: 2faf:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the The Structure of Chicken Mitochondrial Pepck.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of The Structure of Chicken Mitochondrial Pepck. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1701 b:14.9 occ:1.00 OD1 A:ASP330 1.9 18.2 1.0 NZ A:LYS263 2.0 19.2 1.0 NE2 A:HIS283 2.0 16.6 1.0 SG A:CYS307 2.3 31.8 1.0 CG A:ASP330 2.9 18.1 1.0 CE1 A:HIS283 3.0 18.2 1.0 CD2 A:HIS283 3.0 15.0 1.0 OD2 A:ASP330 3.1 21.8 1.0 CE A:LYS263 3.1 21.3 1.0 CB A:CYS307 3.4 34.4 1.0 CA A:CYS307 4.1 34.5 1.0 ND1 A:HIS283 4.1 15.3 1.0 CG A:HIS283 4.1 14.8 1.0 OH7 A:1PE1262 4.2 28.8 0.5 CB A:ASP330 4.3 16.3 1.0 C16 A:1PE1262 4.4 29.7 0.5 O A:HOH2081 4.4 33.6 1.0 CD A:LYS263 4.5 17.1 1.0 O A:HOH1899 4.5 32.0 1.0 OH7 A:1PE1262 4.6 27.1 0.5 C26 A:1PE1262 4.6 29.5 0.5 C A:CYS307 4.7 34.4 1.0 O A:ASP330 4.7 17.2 1.0 N A:GLY308 4.8 34.7 1.0 CA A:ASP330 4.9 17.0 1.0 C16 A:1PE1262 4.9 28.5 0.5 CE2 A:PHE504 5.0 16.0 1.0

Manganese binding site 2 out of 2 in the The Structure of Chicken Mitochondrial Pepck.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of The Structure of Chicken Mitochondrial Pepck. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1702 b:16.7 occ:1.00 OD1 B:ASP330 1.9 19.8 1.0 NZ B:LYS263 2.0 25.5 1.0 NE2 B:HIS283 2.0 20.4 1.0 SG B:CYS307 2.3 28.0 1.0 CG B:ASP330 2.8 20.2 1.0 CD2 B:HIS283 2.9 20.1 1.0 CE B:LYS263 3.0 25.8 1.0 CE1 B:HIS283 3.0 19.3 1.0 OD2 B:ASP330 3.0 21.4 1.0 CB B:CYS307 3.3 31.7 1.0 CA B:CYS307 4.0 31.6 1.0 CG B:HIS283 4.1 19.2 1.0 ND1 B:HIS283 4.1 20.1 1.0 O B:HOH1977 4.2 33.2 1.0 O B:HOH1947 4.2 27.2 1.0 CB B:ASP330 4.3 19.6 1.0 CD B:LYS263 4.4 24.3 1.0 O B:HOH1974 4.5 31.9 1.0 O B:HOH1965 4.6 36.3 1.0 O B:ASP330 4.7 20.2 1.0 C B:CYS307 4.7 30.9 1.0 N B:GLY308 4.8 30.4 1.0 CA B:ASP330 4.8 19.4 1.0

T.Holyoak, S.M.Sullivan, T.Nowak. Structural Insights Into the Mechanism of Pepck Catalysis Biochemistry V. 45 8254 2006.
ISSN: ISSN 0006-2960
PubMed: 16819824
DOI: 10.1021/BI060269G