Manganese in PDB 1o6l: Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide

All present enzymatic activity of Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide:
2.7.1.37;

The structure of Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide, PDB code: 1o6l was solved by J.Yang, P.Cron, V.M.Good, V.Thompson, B.A.Hemmings, D.Barford, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.08 / 1.6
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	44.936, 60.997, 131.315, 90.00, 90.00, 90.00
R / Rfree (%)	19.8 / 22.7

The binding sites of Manganese atom in the Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide (pdb code 1o6l). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide, PDB code: 1o6l:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1481 b:11.4 occ:1.00 O2A A:ANP1480 2.1 12.1 1.0 O2G A:ANP1480 2.1 11.2 1.0 OD1 A:ASN280 2.2 9.2 1.0 O A:HOH2399 2.2 12.7 1.0 OD2 A:ASP293 2.2 11.4 1.0 N3B A:ANP1480 2.8 12.9 1.0 PG A:ANP1480 3.0 11.5 1.0 CG A:ASN280 3.2 10.8 1.0 CG A:ASP293 3.2 10.0 1.0 PA A:ANP1480 3.4 13.4 1.0 ND2 A:ASN280 3.6 9.7 1.0 CB A:ASP293 3.6 9.9 1.0 O1G A:ANP1480 3.8 12.0 1.0 PB A:ANP1480 3.8 13.4 1.0 CE A:LYS277 3.8 11.5 1.0 O3A A:ANP1480 3.9 14.0 1.0 O2B A:ANP1480 3.9 14.3 1.0 MN A:MN1482 4.0 13.1 1.0 O3G A:ANP1480 4.2 11.9 1.0 OD1 A:ASP293 4.3 11.7 1.0 O3' A:ANP1480 4.3 12.8 1.0 NZ A:LYS277 4.3 11.9 1.0 O1A A:ANP1480 4.4 12.1 1.0 OD2 A:ASP275 4.5 11.2 1.0 O5' A:ANP1480 4.5 12.6 1.0 O C:HOH2007 4.5 25.4 1.0 CB A:ASN280 4.5 8.8 1.0 O A:HOH2395 4.6 24.9 1.0 C5' A:ANP1480 4.6 11.9 1.0 C3' A:ANP1480 4.8 12.1 1.0 O A:GLU279 4.8 10.1 1.0 CA A:ASN280 4.8 9.6 1.0 C A:GLU279 5.0 10.7 1.0 N A:ASN280 5.0 9.7 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of An Activated Akt/Protein Kinase B (Pkb-Pif Chimera) Ternary Complex with Amp-Pnp and GSK3 Peptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1482 b:13.1 occ:1.00 O1G A:ANP1480 2.1 12.0 1.0 O2B A:ANP1480 2.2 14.3 1.0 O A:HOH2401 2.2 13.1 1.0 OD1 A:ASP293 2.3 11.7 1.0 OD2 A:ASP293 2.3 11.4 1.0 O A:HOH2400 2.3 18.4 1.0 CG A:ASP293 2.6 10.0 1.0 PG A:ANP1480 3.2 11.5 1.0 PB A:ANP1480 3.3 13.4 1.0 N3B A:ANP1480 3.5 12.9 1.0 O2G A:ANP1480 3.7 11.2 1.0 OG C:SER9 3.9 16.2 1.0 MN A:MN1481 4.0 11.4 1.0 OD2 A:ASP275 4.1 11.2 1.0 O A:HOH2041 4.1 18.9 1.0 CB A:ASP293 4.1 9.9 1.0 O A:HOH2108 4.1 36.4 1.0 O1B A:ANP1480 4.3 16.6 1.0 NZ A:LYS181 4.3 16.7 1.0 CA A:GLY295 4.4 12.8 1.0 O3A A:ANP1480 4.5 14.0 1.0 O A:HOH2180 4.5 25.7 1.0 O3G A:ANP1480 4.5 11.9 1.0 O2A A:ANP1480 4.5 12.1 1.0 N A:GLY295 4.6 12.5 1.0 CB C:SER9 4.6 14.8 1.0 PA A:ANP1480 4.8 13.4 1.0 O A:HOH2398 4.8 33.9 1.0 C A:GLY295 4.9 13.3 1.0 O1A A:ANP1480 4.9 12.1 1.0 CA A:ASP293 4.9 10.6 1.0 O A:ASP293 5.0 11.3 1.0

J.Yang, P.Cron, V.M.Good, V.Thompson, B.A.Hemmings, D.Barford. Crystal Structure of An Activated Akt/Protein Kinase B Ternary Complex with Gsk-3 Peptide and Amp-Pnp Nat.Struct.Biol. V. 9 940 2002.
ISSN: ISSN 1072-8368
PubMed: 12434148
DOI: 10.1038/NSB870