Manganese in PDB 8jbk: Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State

The structure of Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State, PDB code: 8jbk was solved by R.Kanai, B.Vilsen, F.Cornelius, C.Toyoshima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	193.74, 74.49, 162.56, 90, 115.72, 90
R / Rfree (%)	22.9 / 27

The structure of Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State also contains other interesting chemical elements:

Sodium

(Na)

8 atoms

The binding sites of Manganese atom in the Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State (pdb code 8jbk). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State, PDB code: 8jbk:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1101 b:58.8 occ:1.00 OD1 A:ASP710 2.2 68.4 1.0 O A:HOH1212 2.2 62.8 1.0 O A:HOH1207 2.3 63.5 1.0 CG A:ASP710 3.0 60.7 1.0 OD2 A:ASP710 3.1 61.0 1.0 O A:HOH1215 3.3 40.9 1.0 N A:GLY711 4.1 54.4 1.0 OD2 A:ASP714 4.2 49.2 1.0 CB A:ASP710 4.4 54.9 1.0 CA A:GLY711 4.5 51.7 1.0 C A:ASP710 4.7 59.7 1.0 O A:HOH1201 4.8 54.2 1.0 N A:ASP710 4.9 57.4 1.0 CA A:ASP710 4.9 59.0 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Na+,K+-Atpase in the E1.3NA+ State within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn1101 b:69.9 occ:1.00 OD1 C:ASP710 2.2 78.3 1.0 O C:HOH1201 2.2 58.1 1.0 O C:HOH1206 2.3 58.3 1.0 CG C:ASP710 2.9 68.6 1.0 OD2 C:ASP710 3.0 71.6 1.0 OD2 C:ASP714 4.0 72.7 1.0 N C:GLY711 4.2 53.1 1.0 CB C:ASP710 4.4 62.6 1.0 N C:ASP710 4.7 63.1 1.0 CA C:GLY711 4.7 54.3 1.0 C C:ASP710 4.8 49.9 1.0 CA C:ASP710 4.9 58.7 1.0 O C:HOH1208 4.9 38.0 1.0

R.Kanai, B.Vilsen, F.Cornelius, C.Toyoshima. Crystal Structures of Na + ,K + -Atpase Reveal the Mechanism That Converts the K + -Bound Form to Na + -Bound Form and Opens and Closes the Cytoplasmic Gate. Febs Lett. 2023.
ISSN: ISSN 0014-5793
PubMed: 37357620
DOI: 10.1002/1873-3468.14689