Manganese in PDB 7qwj: Crystal Structure of GLC7 Phosphatase

All present enzymatic activity of Crystal Structure of GLC7 Phosphatase:
3.1.3.16;

The structure of Crystal Structure of GLC7 Phosphatase, PDB code: 7qwj was solved by K.Malhotra, M.Carminati, A.Bertolotti, D.Bellini, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.27 / 1.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	38.284, 83.294, 96.532, 90, 90, 90
R / Rfree (%)	21.9 / 26.7

The binding sites of Manganese atom in the Crystal Structure of GLC7 Phosphatase (pdb code 7qwj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of GLC7 Phosphatase, PDB code: 7qwj:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of GLC7 Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of GLC7 Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:20.2 occ:1.00 O3 A:PO4403 2.1 19.8 0.7 OD1 A:ASN123 2.1 21.9 1.0 NE2 A:HIS172 2.2 17.9 1.0 ND1 A:HIS247 2.2 19.4 1.0 OD1 A:ASP91 2.3 21.6 1.0 O2 A:PO4403 2.6 19.2 0.7 P A:PO4403 2.9 32.2 0.7 CE1 A:HIS247 3.0 19.0 1.0 CG A:ASP91 3.1 19.1 1.0 CE1 A:HIS172 3.2 16.1 1.0 CD2 A:HIS172 3.2 18.5 1.0 CG A:ASN123 3.2 20.2 1.0 MN A:MN402 3.4 22.5 1.0 OD2 A:ASP91 3.4 19.3 1.0 CG A:HIS247 3.4 19.9 1.0 ND2 A:ASN123 3.7 19.6 1.0 CA A:HIS247 3.8 17.4 1.0 O4 A:PO4403 3.8 22.8 0.7 CB A:HIS247 3.9 16.7 1.0 O1 A:PO4403 4.1 15.8 0.7 O A:HIS247 4.1 19.6 1.0 OD2 A:ASP63 4.1 22.0 1.0 NE2 A:HIS247 4.2 19.0 1.0 CD2 A:HIS124 4.2 22.6 1.0 ND1 A:HIS172 4.3 18.6 1.0 CG A:HIS172 4.3 19.1 1.0 CB A:ASP91 4.4 18.4 1.0 CD2 A:HIS247 4.4 19.2 1.0 C A:HIS247 4.4 19.8 1.0 N A:ASN123 4.5 19.7 1.0 CB A:ASN123 4.5 23.4 1.0 O A:LEU204 4.7 18.6 1.0 NE2 A:HIS124 4.8 23.1 1.0 N A:HIS247 4.8 17.1 1.0 NH1 A:ARG220 5.0 23.5 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of GLC7 Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of GLC7 Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:22.5 occ:1.00 OD2 A:ASP63 2.0 22.0 1.0 O3 A:PO4403 2.2 19.8 0.7 O A:HOH544 2.2 20.6 1.0 OD1 A:ASP91 2.2 21.6 1.0 NE2 A:HIS65 2.2 23.5 1.0 O4 A:PO4403 2.4 22.8 0.7 P A:PO4403 2.8 32.2 0.7 CE1 A:HIS65 3.1 22.7 1.0 CD2 A:HIS65 3.2 23.8 1.0 CG A:ASP63 3.2 19.6 1.0 CG A:ASP91 3.3 19.1 1.0 MN A:MN401 3.4 20.2 1.0 CB A:ASP91 3.7 18.4 1.0 O2 A:PO4403 3.8 19.2 0.7 CB A:ASP63 3.9 19.8 1.0 O1 A:PO4403 3.9 15.8 0.7 OD1 A:ASP63 4.1 20.4 1.0 O A:HOH560 4.2 27.6 1.0 ND1 A:HIS65 4.2 24.8 1.0 O A:HIS247 4.3 19.6 1.0 CG A:HIS65 4.3 24.2 1.0 OD2 A:ASP91 4.4 19.3 1.0 CE1 A:HIS172 4.5 16.1 1.0 NE2 A:HIS172 4.5 17.9 1.0 CD2 A:HIS124 4.5 22.6 1.0 CE1 A:PHE266 4.5 21.2 1.0 OH A:TYR271 4.6 34.8 1.0 CA A:HIS247 4.6 17.4 1.0 C A:HIS247 4.6 19.8 1.0 NE2 A:HIS124 4.6 23.1 1.0 ND1 A:HIS247 5.0 19.4 1.0

D.Bellini, K.Malhotra, M.Carminati, A.Bertolotti. Crystal Structure of GLC7 Phosphatase To Be Published.