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Manganese in PDB 6dpa: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dpa was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.83 / 1.49
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.199, 37.746, 62.051, 90.00, 96.25, 90.00
R / Rfree (%) 15.5 / 18.6

Other elements in 6dpa:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 4 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C (pdb code 6dpa). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dpa:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 6dpa

Go back to Manganese Binding Sites List in 6dpa
Manganese binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn201

b:28.1
occ:0.90
O A:HOH315 1.9 21.5 1.0
OD2 A:ASP71 2.0 28.5 0.9
OD1 A:ASP192 2.0 35.5 1.0
OP1 b:C5 2.2 36.6 0.7
OP3 b:C5 2.2 30.8 0.3
O A:HOH305 2.3 29.8 0.7
O A:HOH318 2.3 27.8 1.0
OP1 b:C5 2.4 16.3 0.3
P b:C5 2.8 30.1 0.3
CG A:ASP71 3.0 24.6 0.9
HA A:ASP192 3.1 48.8 1.0
CG A:ASP192 3.2 40.2 1.0
H A:TYR193 3.4 46.8 1.0
OD1 A:ASP71 3.5 26.2 0.9
P b:C5 3.5 17.5 0.7
HB2 A:ASP192 3.7 44.6 1.0
CB A:ASP192 3.8 37.2 1.0
CA A:ASP192 3.8 40.7 1.0
MN A:MN202 3.9 26.3 0.9
OP2 b:C5 3.9 16.0 0.3
O5' b:C5 3.9 46.7 0.3
O A:VAL72 3.9 21.3 1.0
OP2 b:G6 3.9 23.4 1.0
OP2 b:C5 4.1 30.9 0.7
K A:K203 4.1 40.5 0.2
OE2 A:GLU188 4.2 57.6 1.0
HG22 A:ILE189 4.2 32.0 1.0
N A:TYR193 4.2 39.0 1.0
OD2 A:ASP192 4.2 36.0 1.0
O5' b:C5 4.3 15.2 0.7
CB A:ASP71 4.3 25.1 0.9
OD2 A:ASP132 4.4 30.6 1.0
C5' b:C5 4.5 23.0 1.0
HD1 A:TYR193 4.5 41.8 1.0
C A:ASP192 4.6 28.5 1.0
O3' B:U4 4.6 23.0 0.7
HB3 A:ASP192 4.7 44.6 1.0
HG21 A:ILE189 4.7 32.0 1.0
HB2 A:TYR193 4.8 54.5 1.0
CG2 A:ILE189 4.8 26.7 1.0
H A:VAL72 4.8 27.5 1.0
HG23 A:ILE189 4.9 32.0 1.0
O b:HOH103 4.9 34.8 0.8
N A:ASP192 4.9 33.2 1.0

Manganese binding site 2 out of 2 in 6dpa

Go back to Manganese Binding Sites List in 6dpa
Manganese binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn202

b:26.3
occ:0.90
OD1 A:ASP71 2.1 26.2 0.9
OE1 A:GLU109 2.3 26.3 1.0
OD1 A:ASP132 2.3 23.0 1.0
OP1 b:C5 2.5 16.3 0.3
OD2 A:ASP132 2.7 30.6 1.0
OP1 b:C5 2.7 36.6 0.7
CG A:ASP132 2.8 22.7 1.0
O3' B:U4 2.9 23.0 0.7
CG A:ASP71 3.0 24.6 0.9
O A:HOH344 3.1 21.8 1.0
CD A:GLU109 3.1 29.7 1.0
OE2 A:GLU109 3.2 22.5 1.0
OD2 A:ASP71 3.4 28.5 0.9
P b:C5 3.5 17.5 0.7
HG12 A:VAL72 3.5 28.2 1.0
H A:VAL72 3.6 27.5 1.0
C3' B:U4 3.7 14.1 0.3
O3' B:U4 3.8 15.1 0.3
O A:HOH318 3.8 27.8 1.0
P b:C5 3.9 30.1 0.3
MN A:MN201 3.9 28.1 0.9
C5' b:C5 4.1 23.0 1.0
C3' B:U4 4.2 20.7 0.7
H A:ASP132 4.2 19.7 1.0
CB A:ASP71 4.2 25.1 0.9
HG21 A:ILE189 4.3 32.0 1.0
O5' b:C5 4.3 15.2 0.7
O2' B:U4 4.3 20.7 0.7
CB A:ASP132 4.3 18.9 1.0
N A:VAL72 4.4 22.9 1.0
OP2 b:C5 4.4 16.0 0.3
HB2 A:SER133 4.4 22.6 1.0
CG1 A:VAL72 4.4 23.5 1.0
C4' B:U4 4.5 20.2 0.7
O5' b:C5 4.5 46.7 0.3
CA A:ASP71 4.5 20.1 1.0
CG A:GLU109 4.5 22.0 1.0
C4' B:U4 4.6 23.5 0.3
O A:HOH315 4.7 21.5 1.0
OP2 b:C5 4.7 30.9 0.7
HB3 A:ASP132 4.7 22.7 1.0
C5' B:U4 4.7 24.3 0.3
C2' B:U4 4.8 14.3 0.3
HG13 A:VAL72 4.8 28.2 1.0
HG3 A:GLU109 4.8 26.4 1.0
HG22 A:ILE189 4.8 32.0 1.0
OP3 b:C5 4.8 30.8 0.3
K A:K214 4.8 27.3 0.2
O A:VAL72 4.8 21.3 1.0
HG2 A:GLU109 4.8 26.4 1.0
HG11 A:VAL72 4.8 28.2 1.0
HB2 A:ASP132 4.8 22.7 1.0
C2' B:U4 4.9 31.0 0.7
N A:ASP132 4.9 16.4 1.0
CG2 A:ILE189 5.0 26.7 1.0
CA A:ASP132 5.0 17.3 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sun Oct 6 04:04:56 2024

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