Atomistry » Manganese » PDB 4ee3-4fo6 » 4ee5
Atomistry »
  Manganese »
    PDB 4ee3-4fo6 »
      4ee5 »

Manganese in PDB 4ee5: Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose

Enzymatic activity of Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose

All present enzymatic activity of Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose:
2.4.1.22; 2.4.1.38; 2.4.1.90;

Protein crystallography data

The structure of Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose, PDB code: 4ee5 was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.02 / 2.20
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 107.303, 195.341, 143.908, 90.00, 90.00, 90.00
R / Rfree (%) 20.3 / 25

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose (pdb code 4ee5). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose, PDB code: 4ee5:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in 4ee5

Go back to Manganese Binding Sites List in 4ee5
Manganese binding site 1 out of 3 in the Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn405

b:48.2
occ:1.00
 O3B A:UDP404 2.1 77.4 1.0
OD2 A:ASP250 2.2 34.3 1.0
NE2 A:HIS343 2.3 45.2 1.0
O1A A:UDP404 2.3 48.9 1.0
NE2 A:HIS340 2.4 36.5 1.0
O A:HOH551 2.8 45.6 1.0
PB A:UDP404 3.0 79.7 1.0
O2B A:UDP404 3.1 78.1 1.0
CG A:ASP250 3.1 36.7 1.0
CE1 A:HIS343 3.2 49.4 1.0
CD2 A:HIS343 3.3 46.4 1.0
O3A A:UDP404 3.3 64.8 1.0
CE1 A:HIS340 3.4 37.8 1.0
PA A:UDP404 3.4 55.1 1.0
CD2 A:HIS340 3.4 36.0 1.0
OD1 A:ASP250 3.6 33.0 1.0
O3' A:UDP404 4.1 40.5 1.0
NH2 A:ARG187 4.1 34.2 1.0
O2A A:UDP404 4.2 55.5 1.0
ND1 A:HIS343 4.3 51.2 1.0
CB A:ASP250 4.4 32.7 1.0
O1B A:UDP404 4.4 75.2 1.0
CG A:HIS343 4.4 49.7 1.0
NH1 A:ARG187 4.4 37.4 1.0
ND1 A:HIS340 4.5 39.3 1.0
CG A:HIS340 4.6 38.8 1.0
O5' A:UDP404 4.6 43.1 1.0
C3' A:UDP404 4.7 33.7 1.0
NZ A:LYS275 4.8 50.1 1.0
CZ A:ARG187 4.8 38.0 1.0
O A:HOH522 4.9 44.0 1.0
C5' A:UDP404 5.0 34.8 1.0

Manganese binding site 2 out of 3 in 4ee5

Go back to Manganese Binding Sites List in 4ee5
Manganese binding site 2 out of 3 in the Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn405

b:38.0
occ:1.00
 NE2 B:HIS340 2.2 32.8 1.0
O1A B:UDP404 2.3 44.5 1.0
NE2 B:HIS343 2.3 34.2 1.0
O3B B:UDP404 2.3 76.8 1.0
OD2 B:ASP250 2.3 32.4 1.0
CE1 B:HIS340 3.1 33.9 1.0
CG B:ASP250 3.2 28.2 1.0
PB B:UDP404 3.2 79.2 1.0
CD2 B:HIS343 3.2 35.7 1.0
CE1 B:HIS343 3.2 30.3 1.0
CD2 B:HIS340 3.3 29.0 1.0
O B:HOH586 3.4 37.2 1.0
O2B B:UDP404 3.4 75.2 1.0
PA B:UDP404 3.4 53.2 1.0
O3A B:UDP404 3.5 63.2 1.0
OD1 B:ASP250 3.6 25.5 1.0
O B:HOH609 3.6 60.5 1.0
NH2 B:ARG187 4.0 23.8 1.0
O2A B:UDP404 4.2 54.4 1.0
O3' B:UDP404 4.2 30.4 1.0
NZ B:LYS275 4.3 45.4 1.0
ND1 B:HIS340 4.3 30.3 1.0
NH1 B:ARG187 4.3 24.5 1.0
CB B:ASP250 4.3 27.7 1.0
ND1 B:HIS343 4.4 39.5 1.0
CG B:HIS340 4.4 32.6 1.0
CG B:HIS343 4.4 34.6 1.0
O1B B:UDP404 4.6 74.3 1.0
CZ B:ARG187 4.7 30.8 1.0
O5' B:UDP404 4.7 44.5 1.0
C3' B:UDP404 4.8 25.9 1.0

Manganese binding site 3 out of 3 in 4ee5

Go back to Manganese Binding Sites List in 4ee5
Manganese binding site 3 out of 3 in the Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-1 (M340H-B4GAL-T1) in Complex with Trisaccharide From Lacto-N- Neotetraose within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mn405

b:55.6
occ:1.00
 OD2 C:ASP250 2.2 42.4 1.0
O3B C:UDP404 2.2 82.4 1.0
NE2 C:HIS343 2.3 55.2 1.0
O1A C:UDP404 2.3 71.9 1.0
NE2 C:HIS340 2.5 56.4 1.0
O C:HOH584 2.8 50.9 1.0
CE1 C:HIS343 3.1 57.9 1.0
CG C:ASP250 3.2 37.9 1.0
PA C:UDP404 3.3 66.7 1.0
CD2 C:HIS340 3.3 54.3 1.0
PB C:UDP404 3.4 85.2 1.0
CD2 C:HIS343 3.4 55.9 1.0
CE1 C:HIS340 3.5 56.2 1.0
O3A C:UDP404 3.5 75.8 1.0
OD1 C:ASP250 3.7 38.2 1.0
O2A C:UDP404 3.9 71.3 1.0
NZ C:LYS275 4.0 62.3 1.0
O2B C:UDP404 4.1 84.7 1.0
O3' C:UDP404 4.2 39.9 1.0
NH2 C:ARG187 4.2 44.7 1.0
ND1 C:HIS343 4.3 53.6 1.0
CG C:HIS343 4.5 56.5 1.0
CB C:ASP250 4.5 36.9 1.0
O1B C:UDP404 4.5 83.8 1.0
CG C:HIS340 4.5 52.9 1.0
ND1 C:HIS340 4.6 57.1 1.0
NH1 C:ARG187 4.6 44.9 1.0
O5' C:UDP404 4.7 63.8 1.0
C3' C:UDP404 4.9 43.5 1.0
CZ C:ARG187 4.9 47.5 1.0

Reference:

B.Ramakrishnan, E.Boeggeman, P.K.Qasba. Binding of N-Acetylglucosamine (Glcnac) Beta 1-6-Branched Oligosaccharide Acceptors to Beta 4-Galactosyltransferase I Reveals A New Ligand Binding Mode. J.Biol.Chem. V. 287 28666 2012.
ISSN: ISSN 0021-9258
PubMed: 22740701
DOI: 10.1074/JBC.M112.373514
Page generated: Sat Oct 5 19:18:57 2024

Last articles

Mg in 1IBR
Mg in 1IAQ
Mg in 1IAH
Mg in 1I9V
Mg in 1I97
Mg in 1IA0
Mg in 1I94
Mg in 1I9T
Mg in 1I9S
Mg in 1I96
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy