Atomistry » Manganese » PDB 2a7a-2axt » 2ae7
Atomistry »
  Manganese »
    PDB 2a7a-2axt »
      2ae7 »

Manganese in PDB 2ae7: Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide

Enzymatic activity of Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide

All present enzymatic activity of Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide:
2.4.1.90;

Protein crystallography data

The structure of Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide, PDB code: 2ae7 was solved by V.Ramasamy, B.Ramakrishnan, E.Boeggeman, D.M.Ratner, P.H.Seeberger, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.00 / 2.00
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 106.982, 195.172, 143.919, 90.00, 90.00, 90.00
R / Rfree (%) 21.9 / 24.6

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide (pdb code 2ae7). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide, PDB code: 2ae7:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in 2ae7

Go back to Manganese Binding Sites List in 2ae7
Manganese binding site 1 out of 3 in the Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn400

b:31.3
occ:1.00
 O3B A:UDH399 2.1 30.7 1.0
OD2 A:ASP250 2.1 25.0 1.0
NE2 A:HIS343 2.2 37.2 1.0
NE2 A:HIS340 2.2 28.6 1.0
O1A A:UDH399 2.2 29.6 1.0
O A:HOH482 2.3 23.7 1.0
CG A:ASP250 3.0 26.2 1.0
CD2 A:HIS343 3.1 36.6 1.0
CE1 A:HIS340 3.1 27.9 1.0
CE1 A:HIS343 3.2 38.4 1.0
CD2 A:HIS340 3.3 27.7 1.0
PB A:UDH399 3.3 32.0 1.0
OD1 A:ASP250 3.4 22.6 1.0
PA A:UDH399 3.4 30.2 1.0
O3A A:UDH399 3.6 30.5 1.0
O2B A:UDH399 3.9 34.6 1.0
O3' A:UDH399 3.9 30.5 1.0
O2A A:UDH399 4.3 31.8 1.0
CG A:HIS343 4.3 38.1 1.0
ND1 A:HIS340 4.3 28.8 1.0
NH2 A:ARG187 4.3 34.7 1.0
CB A:ASP250 4.3 24.8 1.0
ND1 A:HIS343 4.3 38.3 1.0
CG A:HIS340 4.4 29.7 1.0
O A:HOH827 4.5 51.2 1.0
NZ A:LYS275 4.5 39.9 1.0
O5' A:UDH399 4.5 31.4 1.0
NH1 A:ARG187 4.6 35.6 1.0
C5B A:UDH399 4.6 29.8 1.0
C3B A:UDH399 4.7 28.4 1.0
O1B A:UDH399 4.7 32.7 1.0
CZ A:ARG187 4.9 33.6 1.0

Manganese binding site 2 out of 3 in 2ae7

Go back to Manganese Binding Sites List in 2ae7
Manganese binding site 2 out of 3 in the Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn402

b:25.5
occ:1.00
 O3B B:UDH401 2.1 28.8 1.0
O1A B:UDH401 2.2 21.7 1.0
NE2 B:HIS343 2.2 24.9 1.0
NE2 B:HIS340 2.3 23.3 1.0
OD2 B:ASP250 2.3 22.6 1.0
O B:HOH484 2.3 25.0 1.0
CG B:ASP250 3.1 19.5 1.0
CD2 B:HIS343 3.1 26.3 1.0
CD2 B:HIS340 3.2 23.9 1.0
CE1 B:HIS340 3.2 23.8 1.0
CE1 B:HIS343 3.3 26.7 1.0
PB B:UDH401 3.3 29.4 1.0
PA B:UDH401 3.4 25.9 1.0
OD1 B:ASP250 3.4 21.0 1.0
O3A B:UDH401 3.6 27.6 1.0
O2B B:UDH401 3.9 31.7 1.0
O3' B:UDH401 3.9 20.6 1.0
NZ B:LYS275 4.1 38.1 1.0
O2A B:UDH401 4.2 28.4 1.0
NH2 B:ARG187 4.3 21.2 1.0
CG B:HIS343 4.3 26.7 1.0
ND1 B:HIS343 4.3 26.2 1.0
NH1 B:ARG187 4.4 21.2 1.0
ND1 B:HIS340 4.4 23.0 1.0
CB B:ASP250 4.4 20.4 1.0
CG B:HIS340 4.4 23.2 1.0
O5' B:UDH401 4.5 24.0 1.0
C5B B:UDH401 4.7 22.4 1.0
C3B B:UDH401 4.7 19.6 1.0
O1B B:UDH401 4.7 28.2 1.0
CZ B:ARG187 4.8 24.1 1.0
O B:HOH723 5.0 46.3 1.0

Manganese binding site 3 out of 3 in 2ae7

Go back to Manganese Binding Sites List in 2ae7
Manganese binding site 3 out of 3 in the Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Human M340H-BETA1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mn404

b:38.6
occ:1.00
 O3B C:UDH403 2.0 38.2 1.0
OD2 C:ASP250 2.1 32.9 1.0
O C:HOH560 2.1 35.3 1.0
NE2 C:HIS343 2.2 41.1 1.0
O1A C:UDH403 2.3 38.0 1.0
NE2 C:HIS340 2.4 44.0 1.0
CG C:ASP250 3.0 31.8 1.0
CE1 C:HIS343 3.1 41.0 1.0
CD2 C:HIS343 3.2 41.2 1.0
PB C:UDH403 3.3 40.8 1.0
OD1 C:ASP250 3.3 30.7 1.0
CD2 C:HIS340 3.4 44.0 1.0
CE1 C:HIS340 3.4 44.0 1.0
PA C:UDH403 3.4 39.7 1.0
O3A C:UDH403 3.6 40.4 1.0
O2B C:UDH403 3.9 41.5 1.0
O3' C:UDH403 4.0 34.2 1.0
NZ C:LYS275 4.1 45.8 1.0
ND1 C:HIS343 4.2 40.3 1.0
O2A C:UDH403 4.3 43.9 1.0
CG C:HIS343 4.3 41.5 1.0
CB C:ASP250 4.4 30.3 1.0
NH2 C:ARG187 4.4 38.2 1.0
ND1 C:HIS340 4.5 45.2 1.0
O5' C:UDH403 4.5 39.4 1.0
CG C:HIS340 4.5 43.9 1.0
NH1 C:ARG187 4.5 40.9 1.0
C5B C:UDH403 4.6 37.0 1.0
O1B C:UDH403 4.7 37.7 1.0
C3B C:UDH403 4.8 34.4 1.0
CZ C:ARG187 5.0 40.6 1.0

Reference:

V.Ramasamy, B.Ramakrishnan, E.Boeggeman, D.M.Ratner, P.H.Seeberger, P.K.Qasba. Oligosaccharide Preferences of BETA1,4-Galactosyltransferase-I: Crystal Structures of MET340HIS Mutant of Human BETA1,4-Galactosyltransferase-I with A Pentasaccharide and Trisaccharides of the N-Glycan Moiety J.Mol.Biol. V. 353 53 2005.
ISSN: ISSN 0022-2836
PubMed: 16157350
DOI: 10.1016/J.JMB.2005.07.050
Page generated: Sat Oct 5 13:24:06 2024

Last articles

Zn in 9MJ5
Zn in 9HNW
Zn in 9G0L
Zn in 9FNE
Zn in 9DZN
Zn in 9E0I
Zn in 9D32
Zn in 9DAK
Zn in 8ZXC
Zn in 8ZUF
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy