Atomistry » Manganese » PDB 1r2m-1sfy » 1s4p
Atomistry »
  Manganese »
    PDB 1r2m-1sfy »
      1s4p »

Manganese in PDB 1s4p: Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor

Enzymatic activity of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor

All present enzymatic activity of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor:
2.4.1.131;

Protein crystallography data

The structure of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor, PDB code: 1s4p was solved by Y.D.Lobsanov, P.A.Romero, B.Sleno, B.Yu, P.Yip, A.Herscovics, P.L.Howell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.96 / 2.01
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 60.694, 101.068, 62.036, 90.00, 98.81, 90.00
R / Rfree (%) 20.3 / 25.6

Other elements in 1s4p:

The structure of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor also contains other interesting chemical elements:

Chlorine (Cl) 4 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor (pdb code 1s4p). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor, PDB code: 1s4p:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 1s4p

Go back to Manganese Binding Sites List in 1s4p
Manganese binding site 1 out of 2 in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn909

b:24.8
occ:1.00
 NE2 A:HIS388 2.1 21.1 1.0
OE1 A:GLU247 2.2 21.3 1.0
O1A A:GDP904 2.2 25.4 1.0
O2B A:GDP904 2.3 26.3 1.0
O A:HOH939 2.3 21.5 1.0
O A:HOH1156 2.4 18.2 1.0
CE1 A:HIS388 2.8 21.1 1.0
PB A:GDP904 3.3 26.2 1.0
CD2 A:HIS388 3.4 22.2 1.0
PA A:GDP904 3.4 25.2 1.0
CD A:GLU247 3.4 20.6 1.0
O3A A:GDP904 3.6 25.4 1.0
O3B A:GDP904 3.7 26.2 1.0
O A:HOH971 3.9 24.3 1.0
O A:HOH1029 4.0 35.5 1.0
O A:HOH969 4.0 26.3 1.0
ND1 A:HIS388 4.1 20.9 1.0
OE2 A:GLU247 4.1 23.9 1.0
C5' A:GDP904 4.3 23.3 1.0
O5' A:GDP904 4.3 25.2 1.0
CG A:HIS388 4.3 18.5 1.0
CB A:GLU247 4.4 17.2 1.0
CG A:GLU247 4.5 16.4 1.0
O2A A:GDP904 4.5 26.0 1.0
O A:HOH1236 4.6 28.8 1.0
O1B A:GDP904 4.7 27.8 1.0
O A:HOH1050 4.7 34.2 1.0
C3' A:GDP904 4.7 23.8 1.0
O A:HOH994 4.8 28.1 1.0
O A:PRO248 4.9 20.3 1.0
C4' A:GDP904 5.0 23.6 1.0

Manganese binding site 2 out of 2 in 1s4p

Go back to Manganese Binding Sites List in 1s4p
Manganese binding site 2 out of 2 in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Ternary Complex with Gdp/Mn and Methyl-Alpha-Mannoside Acceptor within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn906

b:25.0
occ:1.00
 NE2 B:HIS388 2.2 19.1 1.0
O1A B:GDP905 2.2 22.4 1.0
O2B B:GDP905 2.3 25.5 1.0
OE1 B:GLU247 2.3 21.6 1.0
O B:HOH1141 2.3 17.3 1.0
O B:HOH1140 2.3 22.6 1.0
CE1 B:HIS388 3.0 18.6 1.0
PB B:GDP905 3.3 25.5 1.0
CD2 B:HIS388 3.3 18.2 1.0
PA B:GDP905 3.4 23.2 1.0
CD B:GLU247 3.5 21.2 1.0
O3B B:GDP905 3.5 25.9 1.0
O3A B:GDP905 3.6 23.6 1.0
O B:HOH1010 3.8 24.9 1.0
O B:HOH983 3.9 24.6 1.0
O B:HOH1026 4.0 39.9 1.0
ND1 B:HIS388 4.2 20.3 1.0
OE2 B:GLU247 4.2 27.2 1.0
C5' B:GDP905 4.2 23.3 1.0
O5' B:GDP905 4.3 24.6 1.0
CG B:HIS388 4.4 16.5 1.0
CB B:GLU247 4.4 15.3 1.0
O B:HOH1131 4.5 30.6 1.0
O2A B:GDP905 4.5 25.7 1.0
CG B:GLU247 4.6 21.1 1.0
O1B B:GDP905 4.6 28.1 1.0
O B:HOH1091 4.6 24.6 1.0
C3' B:GDP905 4.7 23.5 1.0
O B:PRO248 4.9 17.6 1.0
O B:HOH978 4.9 42.1 1.0
C4' B:GDP905 5.0 24.9 1.0

Reference:

Y.D.Lobsanov, P.A.Romero, B.Sleno, B.Yu, P.Yip, A.Herscovics, P.L.Howell. Structure of KRE2P/MNT1P: A Yeast {Alpha}1,2-Mannosyltransferase Involved in Mannoprotein Biosynthesis J.Biol.Chem. V. 279 17921 2004.
ISSN: ISSN 0021-9258
PubMed: 14752117
DOI: 10.1074/JBC.M312720200
Page generated: Sat Oct 5 12:22:23 2024

Last articles

La in 5YTQ
La in 6IP9
La in 1DJG
La in 6DAM
La in 5KKB
La in 2OQR
La in 2RPV
La in 5KIJ
La in 2K0J
La in 2I18
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy