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Manganese in PDB 1o23: Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose

Enzymatic activity of Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose

All present enzymatic activity of Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose:
2.4.1.90;

Protein crystallography data

The structure of Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose, PDB code: 1o23 was solved by B.Ramakrishnan, P.S.Shah, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.96 / 2.32
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 55.545, 99.379, 102.568, 90.00, 104.09, 90.00
R / Rfree (%) 20.1 / 26.5

Other elements in 1o23:

The structure of Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose also contains other interesting chemical elements:

Calcium (Ca) 2 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose (pdb code 1o23). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose, PDB code: 1o23:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 1o23

Go back to Manganese Binding Sites List in 1o23
Manganese binding site 1 out of 2 in the Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn807

b:40.4
occ:1.00
OD2 B:ASP254 2.1 40.8 1.0
O1A B:UPG809 2.2 38.4 1.0
O B:HOH919 2.2 40.8 1.0
O2B B:UPG809 2.3 44.4 1.0
NE2 B:HIS347 2.3 52.8 1.0
SD B:MET344 2.8 41.1 1.0
CG B:ASP254 3.1 41.1 1.0
CE1 B:HIS347 3.1 52.7 1.0
CE B:MET344 3.2 40.5 1.0
PA B:UPG809 3.3 43.2 1.0
PB B:UPG809 3.3 43.7 1.0
CD2 B:HIS347 3.3 52.2 1.0
O3A B:UPG809 3.4 44.7 1.0
OD1 B:ASP254 3.4 40.4 1.0
O3B B:UPG809 3.6 47.7 1.0
CG B:MET344 3.8 38.6 1.0
O3C B:UPG809 3.9 43.2 1.0
O B:HOH1017 4.0 47.0 1.0
O2A B:UPG809 4.1 47.2 1.0
ND1 B:HIS347 4.3 51.4 1.0
CB B:ASP254 4.4 38.5 1.0
NH2 B:ARG191 4.4 46.5 1.0
O5C B:UPG809 4.4 39.5 1.0
CG B:HIS347 4.4 53.4 1.0
NH1 B:ARG191 4.5 48.9 1.0
O1B B:UPG809 4.6 45.4 1.0
C3C B:UPG809 4.7 41.8 1.0
C5C B:UPG809 4.7 40.5 1.0
CZ B:ARG191 5.0 46.0 1.0

Manganese binding site 2 out of 2 in 1o23

Go back to Manganese Binding Sites List in 1o23
Manganese binding site 2 out of 2 in the Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Lactose Synthase in the Presence of Udp-Glucose within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mn808

b:37.6
occ:1.00
O1A D:UPG810 2.1 39.9 1.0
OD2 D:ASP656 2.1 35.0 1.0
O2B D:UPG810 2.2 40.2 1.0
O D:HOH907 2.2 43.3 1.0
NE2 D:HIS749 2.3 33.7 1.0
SD D:MET746 2.7 41.1 1.0
CG D:ASP656 3.1 35.5 1.0
CE1 D:HIS749 3.1 34.5 1.0
CE D:MET746 3.3 40.9 1.0
PA D:UPG810 3.3 41.2 1.0
CD2 D:HIS749 3.4 32.1 1.0
OD1 D:ASP656 3.4 37.2 1.0
PB D:UPG810 3.5 44.8 1.0
O3A D:UPG810 3.6 45.5 1.0
CG D:MET746 3.8 40.4 1.0
O3C D:UPG810 3.9 37.6 1.0
O3B D:UPG810 4.0 47.8 1.0
O D:HOH1135 4.0 47.7 1.0
O2A D:UPG810 4.2 45.6 1.0
ND1 D:HIS749 4.3 36.1 1.0
O5C D:UPG810 4.4 43.9 1.0
NH2 D:ARG593 4.4 39.6 1.0
CB D:ASP656 4.4 35.9 1.0
CG D:HIS749 4.4 36.4 1.0
O1B D:UPG810 4.5 48.3 1.0
C5C D:UPG810 4.6 38.0 1.0
NH1 D:ARG593 4.6 38.8 1.0
C3C D:UPG810 4.7 38.9 1.0

Reference:

B.Ramakrishnan, P.S.Shah, P.K.Qasba. Alpha-Lactalbumin (La) Stimulates Milk Beta-1,4-Galactosyltransferase I (Beta 4GAL-T1) to Transfer Glucose From Udp-Glucose to N-Acetylglucosamine. Crystal Structure of Beta 4GAL-T1 X La Complex with Udp-Glc. J.Biol.Chem. V. 276 37665 2001.
ISSN: ISSN 0021-9258
PubMed: 11485999
DOI: 10.1074/JBC.M102458200
Page generated: Sat Oct 5 11:53:42 2024

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