Manganese in PDB 1gx5: Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese

All present enzymatic activity of Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese:
2.7.7.48;

The structure of Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese, PDB code: 1gx5 was solved by S.Bressanelli, F.A.Rey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.78 / 1.7
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	67.076, 96.225, 95.995, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 22.3

The binding sites of Manganese atom in the Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese (pdb code 1gx5). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese, PDB code: 1gx5:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in the Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1533 b:39.6 occ:0.68 O A:HOH2373 2.4 35.4 1.0 O A:THR221 2.4 23.6 1.0 O2A A:GTP1531 2.5 62.4 0.6 O A:HOH2649 2.6 40.1 1.0 O2B A:GTP1531 2.6 59.7 0.6 OD1 A:ASP220 2.9 44.1 1.0 OD2 A:ASP318 3.1 50.0 1.0 O3G A:GTP1531 3.4 53.0 0.6 C A:THR221 3.6 22.9 1.0 PA A:GTP1531 3.7 67.0 0.6 PB A:GTP1531 3.7 59.0 0.6 O3A A:GTP1531 3.7 65.2 0.6 O A:HOH2650 3.8 46.7 1.0 MN A:MN1534 3.8 41.5 0.6 N A:THR221 4.0 27.2 1.0 CG A:ASP220 4.1 49.1 1.0 O3B A:GTP1531 4.2 59.0 0.6 PG A:GTP1531 4.2 58.2 0.6 CA A:THR221 4.3 22.4 1.0 CG A:ASP318 4.3 43.8 1.0 O2G A:GTP1531 4.3 62.2 0.6 N A:PHE224 4.4 24.0 1.0 N A:CYS223 4.4 22.8 1.0 C A:ASP220 4.6 24.8 1.0 CB A:THR221 4.6 22.0 1.0 N A:ARG222 4.6 25.0 1.0 CB A:PHE224 4.7 22.2 1.0 O1A A:GTP1531 4.7 62.8 0.6 O5' A:GTP1531 4.7 66.2 0.6 OD2 A:ASP220 4.7 52.5 1.0 CA A:ARG222 4.8 30.0 1.0 C A:ARG222 4.8 26.8 1.0 O A:HOH2651 4.9 53.0 1.0 CB A:ASP318 4.9 35.3 1.0 CA A:ASP220 4.9 33.3 1.0

Manganese binding site 2 out of 3 in the Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1534 b:41.5 occ:0.62 OD1 A:ASP319 2.1 47.8 1.0 OD2 A:ASP318 2.3 50.0 1.0 O A:HOH2651 2.3 53.0 1.0 O A:HOH2652 2.6 53.5 1.0 O A:HOH2650 2.6 46.7 1.0 O2A A:GTP1531 2.6 62.4 0.6 O5' A:GTP1531 3.0 66.2 0.6 CG A:ASP318 3.0 43.8 1.0 OD1 A:ASP318 3.2 47.3 1.0 CG A:ASP319 3.4 44.8 1.0 PA A:GTP1531 3.4 67.0 0.6 O A:HOH2649 3.6 40.1 1.0 MN A:MN1533 3.8 39.6 0.7 OD2 A:ASP319 4.1 47.3 1.0 O3A A:GTP1531 4.3 65.2 0.6 O A:HOH2520 4.3 63.8 1.0 CB A:ASP318 4.4 35.3 1.0 CB A:ASP319 4.4 30.1 1.0 N A:ASP319 4.4 24.9 1.0 C A:ASP318 4.5 28.2 1.0 CA A:ASP319 4.5 25.3 1.0 O A:ASP318 4.6 32.5 1.0 O1A A:GTP1531 4.6 62.8 0.6 O A:HOH2372 4.7 52.7 1.0 O A:HOH2453 4.8 45.2 1.0 OD1 A:ASP220 4.8 44.1 1.0

Manganese binding site 3 out of 3 in the Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Hepatitis C Virus Rna Polymerase in Complex with Gtp and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1535 b:61.0 occ:0.60 O1G A:GTP1532 1.7 54.1 0.5 O2B A:GTP1532 2.3 54.8 0.5 PG A:GTP1532 2.5 59.0 0.5 O2G A:GTP1532 2.8 57.4 0.5 O3B A:GTP1532 3.1 54.1 0.5 PB A:GTP1532 3.1 38.3 0.5 O3A A:GTP1532 3.6 58.1 0.5 O2' A:GTP1532 3.9 47.0 0.5 O3G A:GTP1532 3.9 55.9 0.5 C2' A:GTP1532 4.1 44.6 0.5 C2' A:GTP1532 4.1 56.2 0.5 O1B A:GTP1532 4.3 52.0 0.5 O1B A:GTP1532 4.5 57.3 0.5 C5' A:GTP1532 4.5 57.4 0.5 O2A A:GTP1532 4.6 49.0 0.5 C3' A:GTP1532 4.6 58.5 0.5 O2' A:GTP1532 4.7 60.4 0.5 C8 A:GTP1532 4.8 53.4 1.0 O5' A:GTP1532 4.9 47.2 0.5

S.Bressanelli, L.Tomei, F.A.Rey, R.De Francesco. A Structural Analysis of the Hepatitis C Virus Rna Polymerase in Complex with Ribonucleotides J.Virol. V. 76 3482 2002.
ISSN: ISSN 0022-538X
PubMed: 11884572
DOI: 10.1128/JVI.76.7.3482-3492.2002