Manganese in PDB 1gx4: Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex

All present enzymatic activity of Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex:
2.4.1.151;

The structure of Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex, PDB code: 1gx4 was solved by E.Boix, Y.Zhang, G.J.Swaminathan, K.Brew, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.46
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.626, 95.245, 95.638, 90.00, 99.09, 90.00
R / Rfree (%)	14.9 / 19.4

The binding sites of Manganese atom in the Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex (pdb code 1gx4). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex, PDB code: 1gx4:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:9.6 occ:1.00 O1B A:UDP401 2.2 11.7 1.0 OD2 A:ASP225 2.2 9.4 1.0 OD1 A:ASP227 2.2 9.4 1.0 O A:HOH674 2.2 10.1 1.0 O1A A:UDP401 2.2 10.0 1.0 OD2 A:ASP227 2.5 9.8 1.0 CG A:ASP227 2.7 10.1 1.0 CG A:ASP225 3.2 9.6 1.0 PB A:UDP401 3.3 10.5 1.0 PA A:UDP401 3.4 10.2 1.0 O3A A:UDP401 3.6 10.7 1.0 CB A:ASP225 3.7 8.9 1.0 O3B A:UDP401 3.8 12.1 1.0 NZ A:LYS359 3.9 10.6 1.0 O A:HOH542 3.9 11.0 0.5 O3' A:UDP401 3.9 9.5 1.0 O A:HOH704 4.2 13.0 1.0 CB A:ASP227 4.2 10.0 1.0 OD1 A:ASP225 4.3 10.2 1.0 C5' A:UDP401 4.4 11.3 1.0 O5' A:UDP401 4.4 11.3 1.0 O A:HOH550 4.5 10.8 1.0 O A:HOH661 4.5 11.5 1.0 O A:HOH725 4.5 28.1 1.0 O2A A:UDP401 4.5 11.1 1.0 O2B A:UDP401 4.6 11.2 1.0 O A:HOH583 4.7 11.0 1.0 C3' A:UDP401 4.7 9.7 1.0 CB A:ALA282 4.9 9.5 1.0 O A:ASP227 4.9 10.6 1.0 C4' A:UDP401 4.9 10.5 1.0 N A:ASP227 5.0 9.7 1.0

Manganese binding site 2 out of 2 in the Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Alpha-,1,3 Galactosyltransferase - N-Acetyl Lactosamine Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn402 b:9.9 occ:1.00 O1B B:UDP401 2.1 10.7 1.0 OD2 B:ASP225 2.2 10.2 1.0 O B:HOH645 2.2 10.4 1.0 OD1 B:ASP227 2.2 9.0 1.0 O1A B:UDP401 2.3 9.2 1.0 OD2 B:ASP227 2.5 9.9 1.0 CG B:ASP227 2.7 10.4 1.0 CG B:ASP225 3.2 8.9 1.0 PB B:UDP401 3.3 10.3 1.0 PA B:UDP401 3.5 10.3 1.0 O3A B:UDP401 3.6 10.5 1.0 CB B:ASP225 3.7 7.8 1.0 O3B B:UDP401 3.7 11.1 1.0 NZ B:LYS359 3.8 10.9 1.0 O B:HOH566 3.8 33.6 1.0 O3' B:UDP401 3.9 9.1 1.0 O B:HOH662 4.2 13.2 1.0 CB B:ASP227 4.2 10.1 1.0 OD1 B:ASP225 4.3 9.8 1.0 O5' B:UDP401 4.4 9.8 1.0 C5' B:UDP401 4.4 9.1 1.0 O B:HOH551 4.5 10.3 1.0 O B:HOH628 4.5 11.8 1.0 O2A B:UDP401 4.5 11.7 1.0 O2B B:UDP401 4.6 10.6 1.0 O B:HOH762 4.7 11.5 1.0 O B:HOH723 4.7 22.3 1.0 C3' B:UDP401 4.7 8.2 1.0 CB B:ALA282 4.8 9.6 1.0 O B:ASP227 4.9 10.1 1.0 C4' B:UDP401 4.9 8.6 1.0

E.Boix, Y.Zhang, G.J.Swaminathan, K.Brew, K.R.Acharya. Structural Basis of Ordered Binding of Donor and Acceptor Substrates to the Retaining Glycosyltransferase, Alpha -1,3 Galactosyltransferase J.Biol.Chem. V. 277 28310 2002.
ISSN: ISSN 0021-9258
PubMed: 12011052
DOI: 10.1074/JBC.M202631200