Manganese in PDB 8uwb: Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase.

The structure of Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase., PDB code: 8uwb was solved by F.Wachter, R.P.Nowak, E.S.Fischer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	128.36 / 3.15
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	83.839, 92.597, 134.657, 83.77, 72.46, 73.46
R / Rfree (%)	22.1 / 26.3

The binding sites of Manganese atom in the Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase. (pdb code 8uwb). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase., PDB code: 8uwb:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn401 b:83.7 occ:1.00 OD1 C:ASN117 2.0 90.5 1.0 ND1 C:HIS241 2.2 94.2 1.0 NE2 C:HIS167 2.4 77.2 1.0 OD2 C:ASP85 2.5 88.2 1.0 MN C:MN402 2.9 149.9 1.0 CG C:ASN117 2.9 90.0 1.0 CE1 C:HIS167 3.0 87.2 1.0 CE1 C:HIS241 3.1 100.7 1.0 CG C:HIS241 3.2 89.8 1.0 CA C:HIS241 3.2 97.6 1.0 ND2 C:ASN117 3.3 97.8 1.0 O C:HIS241 3.3 86.5 1.0 CG C:ASP85 3.4 87.4 1.0 CB C:HIS241 3.6 89.2 1.0 CD2 C:HIS167 3.6 86.2 1.0 OD1 C:ASP85 3.7 82.3 1.0 C C:HIS241 3.7 97.4 1.0 OD2 C:ASP57 3.9 87.3 1.0 NE2 C:HIS241 4.2 95.8 1.0 CB C:ASN117 4.2 82.7 1.0 ND1 C:HIS167 4.3 92.7 1.0 CD2 C:HIS241 4.3 85.0 1.0 N C:HIS241 4.4 91.7 1.0 CD2 C:HIS118 4.5 101.5 1.0 CG C:HIS167 4.6 78.0 1.0 CB C:ASP85 4.6 86.2 1.0 CG C:ASP57 4.7 89.8 1.0 N C:ASN117 4.7 107.6 1.0 NE2 C:HIS59 4.7 76.3 1.0 OD1 C:ASP57 4.8 97.2 1.0

Manganese binding site 2 out of 4 in the Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn402 b:149.9 occ:1.00 NE2 C:HIS59 2.3 76.3 1.0 OD2 C:ASP85 2.4 88.2 1.0 MN C:MN401 2.9 83.7 1.0 CE1 C:HIS59 3.1 86.5 1.0 OD2 C:ASP57 3.2 87.3 1.0 CD2 C:HIS59 3.5 84.7 1.0 CG C:ASP85 3.5 87.4 1.0 OH C:TYR265 3.6 92.0 1.0 O C:HIS241 4.0 86.5 1.0 CD2 C:HIS118 4.1 101.5 1.0 CG C:ASP57 4.2 89.8 1.0 ND1 C:HIS59 4.3 79.3 1.0 NE2 C:HIS118 4.3 107.3 1.0 OD1 C:ASN117 4.3 90.5 1.0 CB C:ASP85 4.4 86.2 1.0 NH2 C:ARG89 4.4 115.4 1.0 OD1 C:ASP85 4.4 82.3 1.0 CG C:HIS59 4.5 78.5 1.0 CZ C:TYR265 4.6 84.3 1.0 C C:HIS241 4.6 97.4 1.0 CB C:ASP57 4.8 86.2 1.0 CA C:HIS241 4.8 97.6 1.0 CE1 C:PHE260 4.8 78.1 1.0 ND2 C:ASN117 4.8 97.8 1.0 ND1 C:HIS241 4.9 94.2 1.0 NE C:ARG89 4.9 112.1 1.0 CE1 C:HIS167 4.9 87.2 1.0 NE2 C:HIS167 4.9 77.2 1.0 OD1 C:ASP57 5.0 97.2 1.0

Manganese binding site 3 out of 4 in the Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase. within 5.0Å range: probe atom residue distance (Å) B Occ F:Mn401 b:83.3 occ:1.00 OD1 F:ASN117 2.1 75.5 1.0 ND1 F:HIS241 2.2 93.0 1.0 NE2 F:HIS167 2.3 90.2 1.0 OD2 F:ASP85 2.5 91.4 1.0 CE1 F:HIS167 2.8 94.6 1.0 MN F:MN402 2.9 152.1 1.0 CG F:ASN117 3.0 74.6 1.0 CE1 F:HIS241 3.1 101.3 1.0 CA F:HIS241 3.1 95.2 1.0 CG F:HIS241 3.2 93.9 1.0 CG F:ASP85 3.3 100.4 1.0 O F:HIS241 3.4 112.2 1.0 CD2 F:HIS167 3.5 99.7 1.0 ND2 F:ASN117 3.5 76.0 1.0 CB F:HIS241 3.5 91.6 1.0 OD1 F:ASP85 3.6 87.7 1.0 C F:HIS241 3.7 106.2 1.0 OD2 F:ASP57 3.9 82.7 1.0 ND1 F:HIS167 4.1 99.1 1.0 NE2 F:HIS241 4.2 96.2 1.0 N F:HIS241 4.2 103.1 1.0 CD2 F:HIS241 4.3 92.4 1.0 CB F:ASN117 4.3 57.4 1.0 CG F:HIS167 4.4 84.1 1.0 CB F:ASP85 4.5 99.2 1.0 CG F:ASP57 4.6 92.7 1.0 CD2 F:HIS118 4.6 85.6 1.0 OD1 F:ASP57 4.7 102.8 1.0 N F:ASN117 4.7 82.0 1.0 NE2 F:HIS59 4.8 77.1 1.0

Manganese binding site 4 out of 4 in the Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of PP2A PPP2R1A-PPP2CA-PPP2R5E Phosphatase. within 5.0Å range: probe atom residue distance (Å) B Occ F:Mn402 b:152.1 occ:1.00 OD2 F:ASP85 2.5 91.4 1.0 NE2 F:HIS59 2.8 77.1 1.0 MN F:MN401 2.9 83.3 1.0 CE1 F:HIS59 3.4 86.7 1.0 OD2 F:ASP57 3.4 82.7 1.0 CG F:ASP85 3.7 100.4 1.0 O F:HIS241 3.8 112.2 1.0 CD2 F:HIS59 3.9 89.6 1.0 OH F:TYR265 3.9 107.1 1.0 CD2 F:HIS118 4.0 85.6 1.0 NH2 F:ARG89 4.1 133.0 1.0 NE2 F:HIS118 4.1 88.2 1.0 OD1 F:ASN117 4.2 75.5 1.0 OD1 F:ASP85 4.4 87.7 1.0 CG F:ASP57 4.4 92.7 1.0 C F:HIS241 4.5 106.2 1.0 ND2 F:ASN117 4.5 76.0 1.0 ND1 F:HIS241 4.6 93.0 1.0 ND1 F:HIS59 4.6 83.6 1.0 CB F:ASP85 4.6 99.2 1.0 CA F:HIS241 4.6 95.2 1.0 CG F:ASN117 4.8 74.6 1.0 NE2 F:HIS167 4.9 90.2 1.0 CG F:HIS59 4.9 93.4 1.0 CZ F:TYR265 4.9 83.6 1.0 NE F:ARG89 5.0 132.3 1.0 CZ F:ARG89 5.0 134.6 1.0 CE1 F:HIS167 5.0 94.6 1.0

F.Wachter, R.P.Nowak, S.Ficarro, J.Marto, E.S.Fischer. Structural Characterization of Methylation-Independent PP2A Assembly Guides ALPHAFOLD2MULTIMER Prediction of Family-Wide PP2A Complexes. J.Biol.Chem. 07268 2024.
ISSN: ESSN 1083-351X
PubMed: 38582449
DOI: 10.1016/J.JBC.2024.107268