Manganese in PDB 8pzm: Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese

The structure of Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese, PDB code: 8pzm was solved by M.C.Simpson, C.M.Czekster, C.J.Harding, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.78 / 1.70
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	182.05, 182.05, 87.57, 90, 90, 120
R / Rfree (%)	17 / 18.9

The binding sites of Manganese atom in the Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese (pdb code 8pzm). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese, PDB code: 8pzm:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn605 b:22.4 occ:1.00 OD2 A:ASP310 2.1 22.8 1.0 O2 A:BES601 2.1 22.4 1.0 OE2 A:GLU371 2.2 22.1 1.0 N2 A:BES601 2.3 23.4 1.0 NZ A:LYS287 2.3 21.7 1.0 OD2 A:ASP292 2.4 23.1 1.0 CG A:ASP310 2.9 23.6 1.0 C2 A:BES601 3.0 20.8 1.0 OD1 A:ASP310 3.0 23.2 1.0 C1 A:BES601 3.1 21.5 1.0 CE A:LYS287 3.2 21.5 1.0 CD A:GLU371 3.2 23.1 1.0 MN A:MN606 3.3 23.7 1.0 CG A:ASP292 3.3 21.2 1.0 OE1 A:GLU371 3.5 22.1 1.0 O3 A:BCT603 3.7 23.3 1.0 CB A:ASP292 3.8 22.3 1.0 O A:THR396 4.2 22.7 1.0 OD1 A:ASP292 4.3 21.3 1.0 C3 A:BES601 4.3 24.0 1.0 CB A:ASP310 4.3 21.9 1.0 C6 A:BES601 4.5 23.1 1.0 O3 A:BES601 4.5 24.0 1.0 CG A:GLU371 4.5 22.5 1.0 CD A:LYS287 4.6 22.8 1.0 CG2 A:ILE289 4.7 21.7 1.0 CB A:ILE289 4.7 23.4 1.0 N A:GLY372 4.8 22.4 1.0 CG1 A:ILE289 4.8 21.2 1.0 O A:ASP369 4.9 21.8 1.0

Manganese binding site 2 out of 2 in the Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Intracellular Leucine Aminopeptidase of Pseudomonas Aeruginosa PA14 Bound to Bestatin Inhibitor and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn606 b:23.7 occ:1.00 OD1 A:ASP369 2.1 22.8 1.0 O A:ASP369 2.2 21.8 1.0 OD2 A:ASP292 2.2 23.1 1.0 OE1 A:GLU371 2.2 22.1 1.0 O2 A:BES601 2.2 22.4 1.0 O3 A:BES601 2.5 24.0 1.0 C3 A:BES601 3.0 24.0 1.0 CG A:ASP292 3.0 21.2 1.0 C2 A:BES601 3.0 20.8 1.0 CG A:ASP369 3.1 22.4 1.0 C A:ASP369 3.1 22.2 1.0 CD A:GLU371 3.1 23.1 1.0 OD1 A:ASP292 3.2 21.3 1.0 MN A:MN605 3.3 22.4 1.0 OE2 A:GLU371 3.4 22.1 1.0 CA A:ASP369 3.5 21.6 1.0 C1 A:BES601 3.8 21.5 1.0 NZ A:LYS299 3.8 23.2 1.0 CB A:ASP369 3.8 21.8 1.0 OD2 A:ASP369 4.0 23.0 1.0 O3 A:BCT603 4.0 23.3 1.0 CE A:LYS299 4.1 23.4 1.0 N1 A:BES601 4.1 23.3 1.0 N2 A:BES601 4.2 23.4 1.0 N A:ALA370 4.3 21.9 1.0 N A:GLU371 4.3 22.2 1.0 CB A:ASP292 4.4 22.3 1.0 CG A:GLU371 4.5 22.5 1.0 ND2 A:ASN342 4.6 22.3 1.0 CA A:ALA370 4.7 22.6 1.0 O1 A:BCT603 4.8 25.3 1.0 C4 A:BES601 4.8 23.5 1.0 CA A:GLY294 4.8 21.1 1.0 N A:ASP369 4.9 22.6 1.0 C A:BCT603 4.9 24.2 1.0 OD2 A:ASP310 4.9 22.8 1.0 CB A:GLU371 4.9 22.3 1.0

M.C.Simpson, C.J.Harding, R.M.Czekster, L.Remmel, B.E.Bode, C.M.Czekster. Unveiling the Catalytic Mechanism of A Processive Metalloaminopeptidase. Biochemistry 2023.
ISSN: ISSN 0006-2960
PubMed: 37924287
DOI: 10.1021/ACS.BIOCHEM.3C00420