Manganese in PDB 8jyi: Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E

All present enzymatic activity of Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E:
3.1.26.4;

The structure of Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E, PDB code: 8jyi was solved by Y.Ito, H.Lu, M.Kitajima, H.Ishikawa, Y.Nakata, Y.Iwatani, T.Hoshino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.62 / 1.92
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	61.693, 61.693, 83.234, 90, 90, 90
R / Rfree (%)	21.7 / 25.3

The structure of Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Manganese atom in the Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E (pdb code 8jyi). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E, PDB code: 8jyi:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:37.0 occ:1.00 OAO A:V9O203 1.8 121.1 1.0 OD1 A:ASP23 2.0 37.7 1.0 OE2 A:GLU58 2.0 55.6 1.0 OD1 A:ASP78 2.0 52.1 1.0 OAP A:V9O203 2.2 134.9 1.0 CG A:ASP78 2.8 56.6 1.0 CD A:GLU58 2.9 47.8 1.0 OD2 A:ASP78 3.0 50.0 1.0 CAN A:V9O203 3.0 158.3 1.0 CG A:ASP23 3.1 40.6 1.0 MN A:MN202 3.1 69.1 1.0 OE1 A:GLU58 3.1 45.0 1.0 CAJ A:V9O203 3.2 153.7 1.0 O A:HOH307 3.4 39.3 1.0 OD2 A:ASP23 3.5 50.7 1.0 CAI A:V9O203 3.6 163.3 1.0 O A:GLY24 3.8 42.0 1.0 CAM A:V9O203 4.1 175.5 1.0 OAZ A:V9O203 4.2 159.8 1.0 CB A:ASP78 4.3 51.8 1.0 N A:GLY24 4.3 38.0 1.0 CG A:GLU58 4.3 41.5 1.0 CB A:ASP23 4.4 34.4 1.0 CAE A:V9O203 4.4 163.4 1.0 CAF A:V9O203 4.6 147.9 1.0 CA A:ASP23 4.6 35.7 1.0 C A:ASP78 4.7 47.5 1.0 C A:GLY24 4.8 41.0 1.0 N A:SER79 4.8 38.5 1.0 CA A:ASP78 4.8 42.1 1.0 NH2 A:ARG147 4.9 78.8 1.0 O A:ASP78 4.9 60.3 1.0 N A:ASP78 4.9 41.0 1.0 OD1 A:ASP139 4.9 74.7 1.0 CAH A:V9O203 4.9 177.3 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn202 b:69.1 occ:1.00 OAZ A:V9O203 2.0 159.8 1.0 OD2 A:ASP23 2.2 50.7 1.0 OD1 A:ASP139 2.2 74.7 1.0 OAO A:V9O203 2.3 121.1 1.0 OD2 A:ASP78 2.5 50.0 1.0 CAM A:V9O203 2.8 175.5 1.0 CAN A:V9O203 2.9 158.3 1.0 CG A:ASP23 3.0 40.6 1.0 MN A:MN201 3.1 37.0 1.0 CG A:ASP139 3.1 71.7 1.0 OD1 A:ASP23 3.1 37.7 1.0 CG A:ASP78 3.5 56.6 1.0 OBJ A:V9O203 3.7 170.8 1.0 CB A:ASP139 3.7 56.9 1.0 OD1 A:ASP78 3.8 52.1 1.0 NH2 A:ARG147 4.0 78.8 1.0 OD2 A:ASP139 4.0 79.1 1.0 CAL A:V9O203 4.1 188.8 1.0 CAI A:V9O203 4.2 163.3 1.0 ND2 A:ASN135 4.3 43.3 1.0 CA A:ASP139 4.3 50.9 1.0 CB A:ASP23 4.3 34.4 1.0 CB A:ALA128 4.4 83.8 1.0 OAP A:V9O203 4.5 134.9 1.0 CBB A:V9O203 4.5 174.4 1.0 CBA A:V9O203 4.7 180.7 1.0 OD1 A:ASN135 4.7 46.1 1.0 CB A:ASP78 4.8 51.8 1.0 CAJ A:V9O203 4.8 153.7 1.0 CG A:ASN135 4.9 44.6 1.0 OE2 A:GLU58 5.0 55.6 1.0 CZ A:ARG147 5.0 85.4 1.0

Y.Ito, H.Lu, M.Kitajima, H.Ishikawa, Y.Nakata, Y.Iwatani, T.Hoshino. Crystal Structure of Engineered Hiv-1 Reverse Transcriptase Rnase H Domain Complexed with Laccaic Acid E To Be Published.