Manganese in PDB 8h2c: Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni

All present enzymatic activity of Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni:
2.5.1.97;

The structure of Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni, PDB code: 8h2c was solved by W.S.Song, M.A.Park, D.U.Ki, S.I.Yoon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.90
Space group	P 4 21 2
Cell size a, b, c (Å), α, β, γ (°)	176.805, 176.805, 99.824, 90, 90, 90
R / Rfree (%)	24.5 / 29.5

The binding sites of Manganese atom in the Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni (pdb code 8h2c). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni, PDB code: 8h2c:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:37.2 occ:1.00 NE2 A:HIS211 2.1 63.2 1.0 NE2 A:HIS232 2.1 67.5 1.0 CE1 A:HIS211 2.6 62.9 1.0 CE1 A:HIS232 3.0 65.4 1.0 CD2 A:HIS232 3.1 64.1 1.0 CD2 A:HIS211 3.3 58.8 1.0 OE1 A:GLU17 3.3 64.2 1.0 OE2 A:GLU17 3.4 65.3 1.0 CD A:GLU17 3.8 61.4 1.0 ND1 A:HIS211 3.8 63.4 1.0 ND1 A:HIS232 4.1 64.9 1.0 CG A:HIS211 4.2 58.9 1.0 CG A:HIS232 4.2 61.8 1.0 OG A:SER209 4.2 35.5 1.0

Manganese binding site 2 out of 4 in the Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn401 b:49.2 occ:1.00 NE2 B:HIS211 2.0 66.5 1.0 NE2 B:HIS232 2.2 38.6 1.0 CE1 B:HIS211 2.5 65.5 1.0 CE1 B:HIS232 3.1 40.3 1.0 CD2 B:HIS232 3.2 39.2 1.0 CD2 B:HIS211 3.2 63.4 1.0 OE2 B:GLU17 3.5 64.7 1.0 OE1 B:GLU17 3.7 40.7 1.0 ND1 B:HIS211 3.7 65.0 1.0 CD B:GLU17 4.0 65.3 1.0 OG B:SER209 4.1 33.4 1.0 CG B:HIS211 4.1 61.9 1.0 ND1 B:HIS232 4.2 40.9 1.0 CG B:HIS232 4.3 39.8 1.0 O B:HOH515 4.6 20.5 1.0

Manganese binding site 3 out of 4 in the Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn401 b:41.4 occ:1.00 NE2 C:HIS211 2.1 58.3 1.0 NE2 C:HIS232 2.3 44.8 1.0 CE1 C:HIS211 2.6 57.2 1.0 CE1 C:HIS232 3.1 47.2 1.0 OE2 C:GLU17 3.2 57.5 1.0 CD2 C:HIS232 3.3 45.1 1.0 OE1 C:GLU17 3.4 54.3 1.0 CD2 C:HIS211 3.4 54.8 1.0 CD C:GLU17 3.7 54.0 1.0 ND1 C:HIS211 3.9 57.0 1.0 OG C:SER209 4.2 46.0 1.0 ND1 C:HIS232 4.2 47.8 1.0 CG C:HIS211 4.3 54.5 1.0 CG C:HIS232 4.3 45.3 1.0

Manganese binding site 4 out of 4 in the Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn401 b:28.9 occ:1.00 NE2 D:HIS211 1.9 40.2 1.0 NE2 D:HIS232 2.2 37.5 1.0 CE1 D:HIS211 2.5 38.4 1.0 CE1 D:HIS232 3.1 38.7 1.0 CD2 D:HIS211 3.2 39.1 1.0 CD2 D:HIS232 3.3 37.6 1.0 OE2 D:GLU17 3.7 49.5 1.0 ND1 D:HIS211 3.7 39.0 1.0 OE1 D:GLU17 3.7 50.2 1.0 CG D:HIS211 4.1 39.5 1.0 CD D:GLU17 4.1 48.1 1.0 ND1 D:HIS232 4.2 38.9 1.0 OG D:SER209 4.2 36.4 1.0 CG D:HIS232 4.3 37.9 1.0 CE2 D:PHE246 5.0 37.1 1.0

W.S.Song, M.A.Park, D.U.Ki, S.I.Yoon. Structural Analysis of the Pseudaminic Acid Synthase Psei From Campylobacter Jejuni. Biochem.Biophys.Res.Commun. V. 635 252 2022.
ISSN: ESSN 1090-2104
PubMed: 36283338
DOI: 10.1016/J.BBRC.2022.10.050