Manganese in PDB 8gip: Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+

All present enzymatic activity of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+:
2.7.7.86;

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+, PDB code: 8gip was solved by S.Wu, J.Sohn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.79 / 2.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	77.933, 98.397, 142.47, 90, 90, 90
R / Rfree (%)	18.6 / 24.2

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ also contains other interesting chemical elements:

Zinc	(Zn)	2 atoms
Magnesium	(Mg)	2 atoms

The binding sites of Manganese atom in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ (pdb code 8gip). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+, PDB code: 8gip:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn604 b:66.0 occ:1.00 OE2 A:GLU211 2.2 44.9 1.0 OD2 A:ASP307 2.4 50.3 1.0 OD1 A:ASP213 2.5 46.2 1.0 O2A A:ATP601 2.5 39.7 1.0 CD A:GLU211 3.2 42.1 1.0 PA A:ATP601 3.4 32.5 1.0 CG A:ASP307 3.5 47.1 1.0 CG A:ASP213 3.5 42.2 1.0 O1A A:ATP601 3.6 42.0 1.0 OE1 A:GLU211 3.6 40.5 1.0 MG A:MG602 3.8 39.2 1.0 OD2 A:ASP213 3.8 41.8 1.0 O5' A:ATP601 3.9 36.4 1.0 CB A:ASP307 4.1 43.0 1.0 C5' A:ATP601 4.3 37.9 1.0 OD1 A:ASP307 4.4 48.6 1.0 CG A:GLU211 4.5 40.1 1.0 O3G A:ATP601 4.6 42.8 1.0 O A:HOH719 4.7 46.8 1.0 O3A A:ATP601 4.7 37.2 1.0 CB A:ASP213 4.8 39.1 1.0

Manganese binding site 2 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn604 b:72.5 occ:1.00 OE2 C:GLU211 2.2 49.9 1.0 OD1 C:ASP213 2.3 47.5 1.0 OD2 C:ASP307 2.4 56.1 1.0 O2A C:ATP601 2.8 43.5 1.0 CD C:GLU211 3.2 50.1 1.0 CG C:ASP307 3.2 53.2 1.0 CG C:ASP213 3.3 45.8 1.0 PA C:ATP601 3.5 41.1 1.0 OE1 C:GLU211 3.6 47.4 1.0 OD2 C:ASP213 3.7 47.2 1.0 CB C:ASP307 3.8 49.7 1.0 O1A C:ATP601 3.8 42.0 1.0 O5' C:ATP601 3.9 39.5 1.0 OD1 C:ASP307 4.1 54.7 1.0 C5' C:ATP601 4.2 41.8 1.0 CG C:GLU211 4.5 41.5 1.0 CB C:ASP213 4.6 45.3 1.0 MG C:MG602 4.8 42.2 1.0 O3A C:ATP601 5.0 44.5 1.0 O3G C:ATP601 5.0 41.3 1.0

S.Wu, S.B.Gabelli, J.Sohn. The Structural Basis For 2'-5'/3'-5'-Cgamp Synthesis By Cgas Nat Commun V. 15 4012 2024.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-024-48365-3