Manganese in PDB 8gin: Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+

All present enzymatic activity of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+:
2.7.7.86;

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+, PDB code: 8gin was solved by S.Wu, J.Sohn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.78 / 2.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	77.392, 98.709, 142.26, 90, 90, 90
R / Rfree (%)	18.1 / 22.5

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Zinc	(Zn)	2 atoms

The binding sites of Manganese atom in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ (pdb code 8gin). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+, PDB code: 8gin:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn603 b:72.8 occ:1.00 OD2 A:ASP307 2.4 57.7 1.0 OE2 A:GLU211 2.4 49.7 1.0 OD1 A:ASP213 2.5 49.3 1.0 O2A A:ATP601 2.5 45.6 1.0 PA A:ATP601 3.2 41.6 1.0 O1A A:ATP601 3.4 46.2 1.0 CD A:GLU211 3.4 47.3 1.0 CG A:ASP213 3.5 46.2 1.0 CG A:ASP307 3.6 55.2 1.0 O5' A:ATP601 3.6 40.4 1.0 OE1 A:GLU211 3.8 49.1 1.0 OD2 A:ASP213 3.8 46.3 1.0 C5' A:ATP601 4.0 46.1 1.0 CB A:ASP307 4.2 50.9 1.0 MG A:MG604 4.4 39.5 1.0 O3G A:ATP601 4.5 44.4 1.0 OD1 A:ASP307 4.6 56.4 1.0 O3A A:ATP601 4.6 47.4 1.0 CB A:ASP213 4.7 46.0 1.0 CG A:GLU211 4.7 46.4 1.0

Manganese binding site 2 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn603 b:84.6 occ:1.00 OD2 C:ASP307 2.4 60.3 1.0 O2A C:ATP601 2.5 50.3 1.0 OD1 C:ASP213 2.5 56.7 1.0 OE2 C:GLU211 2.6 54.7 1.0 PA C:ATP601 3.1 46.9 1.0 O1A C:ATP601 3.2 52.9 1.0 CG C:ASP213 3.5 53.1 1.0 CD C:GLU211 3.6 55.9 1.0 CG C:ASP307 3.6 55.9 1.0 O5' C:ATP601 3.6 45.8 1.0 OD2 C:ASP213 3.7 52.0 1.0 OE1 C:GLU211 3.9 57.7 1.0 C5' C:ATP601 4.0 51.7 1.0 CB C:ASP307 4.3 51.9 1.0 O3A C:ATP601 4.5 54.5 1.0 OD1 C:ASP307 4.6 56.8 1.0 MG C:MG604 4.7 48.4 1.0 O3G C:ATP601 4.8 50.1 1.0 CB C:ASP213 4.8 51.1 1.0 CG C:GLU211 4.8 46.9 1.0

S.Wu, S.B.Gabelli, J.Sohn. The Structural Basis For 2'-5'/3'-5'-Cgamp Synthesis By Cgas Nat Commun V. 15 4012 2024.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-024-48365-3