Manganese in PDB 8ecy: Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex

All present enzymatic activity of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex:
2.3.1.225; 6.3.1.2;

The structure of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex also contains other interesting chemical elements:

Chlorine	(Cl)	11 atoms
Calcium	(Ca)	5 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20;

Binding sites:

The binding sites of Manganese atom in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex (pdb code 8ecy). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 20 binding sites of Manganese where determined in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex, PDB code: 8ecy:
Jump to Manganese binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Manganese binding site 1 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:28.5 occ:1.00 OE2 A:GLU203 2.1 73.8 1.0 OE2 A:GLU136 2.4 38.7 1.0 OE2 A:GLU196 2.8 44.1 1.0 CD A:GLU203 3.3 52.2 1.0 CD A:GLU196 3.4 35.7 1.0 CD A:GLU136 3.5 33.5 1.0 OE1 A:GLU196 3.7 41.6 1.0 OE1 A:GLU203 3.9 56.0 1.0 CG A:GLU136 4.1 29.1 1.0 OE1 A:GLU134 4.2 52.5 1.0 O A:HOH639 4.3 24.9 1.0 O A:HOH584 4.3 50.0 1.0 OE1 A:GLU136 4.4 37.5 1.0 CG A:GLU196 4.5 31.6 1.0 CG A:GLU203 4.5 42.6 1.0 CB A:GLU203 4.8 32.6 1.0 ND2 A:ASN194 5.0 26.4 1.0

Manganese binding site 2 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:108.4 occ:1.00 OE2 A:GLU338 2.2 51.9 1.0 OE1 A:GLU134 2.7 52.5 1.0 OE2 A:GLU134 2.9 53.4 1.0 CD A:GLU338 3.1 42.5 1.0 CD A:GLU134 3.1 46.5 1.0 ND1 A:HIS253 3.4 38.8 1.0 OE1 A:GLU338 3.5 48.3 1.0 O A:HOH519 3.5 26.2 1.0 NH1 A:ARG340 4.0 44.5 1.0 CB A:HIS253 4.2 33.7 1.0 CG A:HIS253 4.2 38.9 1.0 CE1 A:HIS253 4.3 40.2 1.0 CG A:GLU338 4.3 39.6 1.0 NH1 A:ARG324 4.4 52.6 1.0 CG A:GLU134 4.6 38.2 1.0

Manganese binding site 3 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Mn401 b:48.2 occ:1.00 OE2 J:GLU203 2.1 73.8 1.0 OE2 J:GLU136 2.4 38.7 1.0 OE2 J:GLU196 2.8 44.1 1.0 CD J:GLU203 3.2 52.2 1.0 CD J:GLU196 3.4 35.7 1.0 CD J:GLU136 3.4 33.5 1.0 OE1 J:GLU196 3.7 41.6 1.0 OE1 J:GLU203 3.8 56.0 1.0 CG J:GLU136 4.0 29.1 1.0 OE1 J:GLU134 4.2 52.5 1.0 O J:HOH605 4.3 50.0 1.0 OE1 J:GLU136 4.4 37.5 1.0 CG J:GLU203 4.4 42.6 1.0 CG J:GLU196 4.4 31.6 1.0 CB J:GLU203 4.7 32.6 1.0 ND2 J:ASN194 5.0 26.4 1.0

Manganese binding site 4 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Mn402 b:100.1 occ:1.00 OE2 J:GLU338 2.1 51.9 1.0 OE1 J:GLU134 2.9 52.5 1.0 CD J:GLU338 3.0 42.5 1.0 ND1 J:HIS253 3.1 38.8 1.0 OE2 J:GLU134 3.1 53.4 1.0 CD J:GLU134 3.3 46.5 1.0 OE1 J:GLU338 3.4 48.3 1.0 NH1 J:ARG340 3.7 44.5 1.0 O J:HOH511 3.7 25.1 1.0 CE1 J:HIS253 4.1 40.2 1.0 CG J:HIS253 4.1 38.9 1.0 CB J:HIS253 4.1 33.7 1.0 CG J:GLU338 4.3 39.6 1.0 NH1 J:ARG324 4.4 52.6 1.0 CZ J:ARG340 4.8 47.2 1.0 CG J:GLU134 4.8 38.2 1.0

Manganese binding site 5 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ L:Mn401 b:28.7 occ:1.00 OE2 L:GLU203 2.1 73.8 1.0 OE2 L:GLU136 2.4 38.7 1.0 O L:HOH505 2.6 25.2 1.0 OE2 L:GLU196 2.8 44.1 1.0 CD L:GLU203 3.3 52.2 1.0 CD L:GLU196 3.4 35.7 1.0 CD L:GLU136 3.4 33.5 1.0 OE1 L:GLU196 3.7 41.6 1.0 OE1 L:GLU203 3.8 56.0 1.0 CG L:GLU136 4.0 29.1 1.0 OE1 L:GLU134 4.3 52.5 1.0 O L:HOH579 4.4 50.0 1.0 OE1 L:GLU136 4.4 37.5 1.0 O L:HOH624 4.4 25.2 1.0 CG L:GLU196 4.4 31.6 1.0 CG L:GLU203 4.4 42.6 1.0 CB L:GLU203 4.7 32.6 1.0 ND2 L:ASN194 4.9 26.4 1.0

Manganese binding site 6 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 6 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ L:Mn402 b:87.5 occ:1.00 OE2 L:GLU338 2.1 51.9 1.0 OE1 L:GLU134 2.8 52.5 1.0 OE2 L:GLU134 2.9 53.4 1.0 CD L:GLU338 3.0 42.5 1.0 CD L:GLU134 3.2 46.5 1.0 ND1 L:HIS253 3.4 38.8 1.0 O L:HOH510 3.4 26.9 1.0 OE1 L:GLU338 3.4 48.3 1.0 NH1 L:ARG340 3.9 44.5 1.0 O L:HOH505 4.2 25.2 1.0 CB L:HIS253 4.2 33.7 1.0 CG L:GLU338 4.2 39.6 1.0 CG L:HIS253 4.2 38.9 1.0 CE1 L:HIS253 4.3 40.2 1.0 NH1 L:ARG324 4.3 52.6 1.0 CG L:GLU134 4.6 38.2 1.0

Manganese binding site 7 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 7 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ Q:Mn401 b:37.1 occ:1.00 OE2 Q:GLU203 2.1 73.8 1.0 OE2 Q:GLU136 2.4 38.7 1.0 OE2 Q:GLU196 2.8 44.1 1.0 CD Q:GLU203 3.2 52.2 1.0 CD Q:GLU196 3.4 35.7 1.0 CD Q:GLU136 3.4 33.5 1.0 OE1 Q:GLU196 3.7 41.6 1.0 OE1 Q:GLU203 3.8 56.0 1.0 CG Q:GLU136 4.0 29.1 1.0 OE1 Q:GLU134 4.2 52.5 1.0 O Q:HOH591 4.3 50.0 1.0 OE1 Q:GLU136 4.3 37.5 1.0 O Q:HOH636 4.3 24.9 1.0 CG Q:GLU203 4.4 42.6 1.0 CG Q:GLU196 4.4 31.6 1.0 CB Q:GLU203 4.7 32.6 1.0 ND2 Q:ASN194 5.0 26.4 1.0

Manganese binding site 8 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 8 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ Q:Mn402 b:87.5 occ:1.00 OE2 Q:GLU338 2.4 51.9 1.0 OE2 Q:GLU134 2.8 53.4 1.0 OE1 Q:GLU134 3.0 52.5 1.0 CD Q:GLU338 3.1 42.5 1.0 CD Q:GLU134 3.2 46.5 1.0 OE1 Q:GLU338 3.4 48.3 1.0 ND1 Q:HIS253 3.9 38.8 1.0 NH1 Q:ARG324 4.0 52.6 1.0 NH1 Q:ARG340 4.2 44.5 1.0 CG Q:GLU338 4.3 39.6 1.0 CB Q:HIS253 4.6 33.7 1.0 CG Q:HIS253 4.7 38.9 1.0 CG Q:GLU134 4.7 38.2 1.0 CZ Q:ARG324 4.8 57.3 1.0 CE1 Q:HIS253 4.8 40.2 1.0

Manganese binding site 9 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 9 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ H:Mn401 b:59.9 occ:1.00 OE2 H:GLU203 2.0 73.8 1.0 OE2 H:GLU136 2.3 38.7 1.0 OE2 H:GLU196 2.7 44.1 1.0 CD H:GLU203 3.2 52.2 1.0 CD H:GLU196 3.3 35.7 1.0 CD H:GLU136 3.3 33.5 1.0 OE1 H:GLU196 3.6 41.6 1.0 OE1 H:GLU203 3.8 56.0 1.0 CG H:GLU136 3.9 29.1 1.0 O H:HOH588 4.2 50.0 1.0 OE1 H:GLU136 4.2 37.5 1.0 CG H:GLU196 4.3 31.6 1.0 O H:HOH628 4.3 25.5 1.0 OE1 H:GLU134 4.3 52.5 1.0 CG H:GLU203 4.3 42.6 1.0 CB H:GLU203 4.6 32.6 1.0 ND2 H:ASN194 4.9 26.4 1.0

Manganese binding site 10 out of 20 in the Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 10 of Cryoem Structure of Bovine Bestrophin-2 and Glutamine Synthetase Complex within 5.0Å range: probe atom residue distance (Å) B Occ H:Mn402 b:102.2 occ:1.00 OE2 H:GLU338 2.2 51.9 1.0 OE1 H:GLU134 2.7 52.5 1.0 ND1 H:HIS253 2.8 38.8 1.0 CD H:GLU338 3.3 42.5 1.0 OE2 H:GLU134 3.3 53.4 1.0 CD H:GLU134 3.4 46.5 1.0 NH1 H:ARG340 3.6 44.5 1.0 CE1 H:HIS253 3.7 40.2 1.0 OE1 H:GLU338 3.7 48.3 1.0 CG H:HIS253 3.8 38.9 1.0 CB H:HIS253 4.0 33.7 1.0 CG H:GLU338 4.6 39.6 1.0 CZ H:ARG340 4.6 47.2 1.0 CG H:GLU134 4.8 38.2 1.0 NH1 H:ARG324 4.9 52.6 1.0 NE2 H:HIS253 4.9 40.8 1.0 CD2 H:HIS253 4.9 39.3 1.0

A.P.Owji, K.Yu, A.Kittredge, J.Wang, Y.Zhang, T.Yang. Bestrophin-2 and Glutamine Synthetase Form A Complex For Glutamate Release. Nature V. 611 180 2022.
ISSN: ESSN 1476-4687
PubMed: 36289327
DOI: 10.1038/S41586-022-05373-X