Manganese in PDB 8e6n: X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State

The structure of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State, PDB code: 8e6n was solved by A.T.Bozzi, S.Ray, C.M.Zimanyi, J.M.Nicoludis, R.Gaudet, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.19 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	105.758, 80.389, 51.754, 90, 94.72, 90
R / Rfree (%)	22.4 / 27.1

The binding sites of Manganese atom in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State (pdb code 8e6n). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State, PDB code: 8e6n:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:88.6 occ:0.70 OD1 A:ASP56 2.4 84.0 1.0 O A:ALA53 2.4 65.3 1.0 O A:HOH625 2.6 62.8 1.0 O A:HOH631 2.7 60.8 1.0 SD A:MET230 3.0 84.9 1.0 CG A:ASP56 3.2 59.4 1.0 OD1 A:ASN59 3.3 68.4 1.0 CE A:MET230 3.4 61.7 1.0 C A:ALA53 3.6 64.2 1.0 CA A:TYR54 3.7 54.7 1.0 CG A:MET230 3.7 66.8 1.0 OD2 A:ASP56 3.7 72.4 1.0 CG A:ASN59 4.1 60.9 1.0 N A:TYR54 4.1 58.6 1.0 ND2 A:ASN59 4.1 51.7 1.0 O A:THR228 4.2 71.2 1.0 C A:TYR54 4.2 57.3 1.0 CB A:ASP56 4.3 50.5 1.0 N A:ASP56 4.5 54.9 1.0 O A:TYR54 4.6 53.9 1.0 O A:HOH602 4.7 80.8 1.0 CB A:TYR54 4.8 55.7 1.0 N A:MET55 4.8 52.4 1.0 CA A:ALA53 4.8 56.3 1.0 CD2 A:TYR54 4.8 43.6 1.0 CG A:TYR54 4.9 57.4 1.0 CA A:ASP56 5.0 52.4 1.0

Manganese binding site 2 out of 2 in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter G223W Mutant in An Outward-Open, Manganese-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn502 b:84.3 occ:0.50 O A:HOH620 2.3 61.0 1.0 NE2 A:HIS287 2.4 79.2 1.0 CD2 A:HIS287 3.2 71.2 1.0 CE1 A:HIS287 3.4 72.2 1.0 CG A:HIS287 4.3 67.8 1.0 ND1 A:HIS287 4.4 68.4 1.0

S.Ray, S.P.Berry, E.A.Wilson, C.H.Zhang, M.Shekhar, A.Singharoy, R.Gaudet. High-Resolution Structures with Bound Mn 2+ and Cd 2+ Map the Metal Import Pathway in An Nramp Transporter. Elife V. 12 2023.
ISSN: ESSN 2050-084X
PubMed: 37039477
DOI: 10.7554/ELIFE.84006