Manganese in PDB 8d3q: Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer

All present enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer:
3.1.12.1;

The structure of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer also contains other interesting chemical elements:

Iron

(Fe)

8 atoms

The binding sites of Manganese atom in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer (pdb code 8d3q). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer, PDB code: 8d3q:

Manganese binding site 1 out of 1 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range: probe atom residue distance (Å) B Occ I:Mn302 b:139.2 occ:1.00 OE1 I:GLU108 1.8 110.3 1.0 N I:TYR109 1.9 106.8 1.0 C I:GLU108 2.0 110.3 1.0 C I:TYR109 2.1 106.8 1.0 CA I:TYR109 2.2 106.8 1.0 O I:GLU108 2.2 110.3 1.0 CD I:GLU108 2.3 110.3 1.0 O I:TYR109 2.5 106.8 1.0 N I:LYS110 2.7 91.4 1.0 OE2 I:GLU108 2.9 110.3 1.0 CA I:GLU108 3.2 110.3 1.0 CG I:GLU108 3.2 110.3 1.0 CB I:GLU108 3.3 110.3 1.0 CG I:LYS110 3.4 91.4 1.0 CB I:TYR109 3.7 106.8 1.0 CA I:LYS110 3.7 91.4 1.0 CB I:LYS110 3.8 91.4 1.0 O I:PHE145 4.1 102.7 1.0 N I:GLU108 4.2 110.3 1.0 OD2 I:ASP82 4.2 110.4 1.0 CD I:LYS110 4.6 91.4 1.0 CG I:TYR109 4.6 106.8 1.0 CD1 I:TYR109 4.7 106.8 1.0 OP1 H:DG29 4.7 85.8 1.0 CE I:LYS110 4.9 91.4 1.0 C I:LYS110 5.0 91.4 1.0

Y.Dhingra, S.K.Suresh, P.Juneja, D.G.Sashital. Pam Binding Ensures Orientational Integration During CAS4-CAS1-CAS2-Mediated Crispr Adaptation. Mol.Cell V. 82 4353 2022.
ISSN: ISSN 1097-2765
PubMed: 36272411
DOI: 10.1016/J.MOLCEL.2022.09.030