Manganese in PDB 8a8f: Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2

All present enzymatic activity of Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2:
3.1.3.16;

The structure of Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2, PDB code: 8a8f was solved by M.Carminati, C.M.Manav, D.Bellini, L.A.Passmore, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.12 / 1.85
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	90.23, 90.23, 101.14, 90, 90, 120
R / Rfree (%)	19.2 / 22.1

The binding sites of Manganese atom in the Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2 (pdb code 8a8f). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2, PDB code: 8a8f:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:38.0 occ:0.80 O A:HOH507 1.7 42.6 1.0 OD1 A:ASP63 2.1 40.7 1.0 O1 A:PO4403 2.1 35.8 0.8 NE2 A:HIS65 2.1 42.4 1.0 OD2 A:ASP91 2.2 35.1 1.0 O A:HOH572 2.3 43.4 1.0 CE1 A:HIS65 3.0 43.2 1.0 CG A:ASP91 3.2 42.7 1.0 P A:PO4403 3.2 39.5 0.8 CD2 A:HIS65 3.2 44.5 1.0 MN A:MN402 3.2 37.8 1.0 CG A:ASP63 3.3 43.3 1.0 O2 A:PO4403 3.5 36.5 0.8 CB A:ASP91 3.6 42.9 1.0 O4 A:PO4403 3.9 37.7 0.8 O A:HOH505 4.0 48.1 1.0 NH1 A:ARG95 4.0 48.5 1.0 OD2 A:ASP63 4.2 48.3 1.0 ND1 A:HIS65 4.2 42.8 1.0 CB A:ASP63 4.3 44.9 1.0 OD1 A:ASP91 4.3 41.8 1.0 CG A:HIS65 4.3 43.4 1.0 CD2 A:HIS124 4.3 40.6 1.0 OH A:TYR271 4.4 52.7 1.0 O A:HIS247 4.5 40.1 1.0 O3 A:PO4403 4.5 39.2 0.8 NE2 A:HIS172 4.5 37.9 1.0 CE1 A:HIS172 4.5 40.1 1.0 NE2 A:HIS124 4.6 41.2 1.0 CA A:HIS247 4.6 40.8 1.0 CE1 A:PHE266 4.7 44.8 1.0 C A:HIS247 4.8 45.3 1.0 OD1 A:ASN123 4.9 40.7 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:37.8 occ:1.00 OD2 A:ASP91 2.0 35.1 1.0 OD1 A:ASN123 2.1 40.7 1.0 O2 A:PO4403 2.2 36.5 0.8 NE2 A:HIS172 2.2 37.9 1.0 O A:HOH507 2.2 42.6 1.0 ND1 A:HIS247 2.3 36.8 1.0 CG A:ASP91 3.1 42.7 1.0 CG A:ASN123 3.1 38.8 1.0 CE1 A:HIS172 3.1 40.1 1.0 CE1 A:HIS247 3.1 38.7 1.0 CD2 A:HIS172 3.2 35.6 1.0 MN A:MN401 3.2 38.0 0.8 P A:PO4403 3.3 39.5 0.8 CG A:HIS247 3.3 37.6 1.0 OD1 A:ASP91 3.5 41.8 1.0 ND2 A:ASN123 3.5 40.8 1.0 O1 A:PO4403 3.6 35.8 0.8 CB A:HIS247 3.7 41.0 1.0 CA A:HIS247 3.8 40.8 1.0 OD1 A:ASP63 4.0 40.7 1.0 O4 A:PO4403 4.1 37.7 0.8 O A:HIS247 4.1 40.1 1.0 CD2 A:HIS124 4.3 40.6 1.0 ND1 A:HIS172 4.3 37.3 1.0 NE2 A:HIS247 4.3 39.6 1.0 CG A:HIS172 4.3 37.4 1.0 CD2 A:HIS247 4.4 38.5 1.0 CB A:ASP91 4.5 42.9 1.0 C A:HIS247 4.5 45.3 1.0 O3 A:PO4403 4.5 39.2 0.8 CB A:ASN123 4.5 35.4 1.0 N A:ASN123 4.6 34.7 1.0 O A:LEU204 4.8 38.8 1.0 N A:HIS247 4.8 41.4 1.0 NE2 A:HIS124 4.9 41.2 1.0 O A:HOH572 5.0 43.4 1.0 NH1 A:ARG220 5.0 38.7 1.0

M.Carminati, C.M.Manav, D.Bellini, L.A.Passmore. Crystal Structure of GLC7 Phosphatase in Complex with the Regulatory Region of REF2 To Be Published.