Manganese in PDB 7zyz: Cryo-Em Structure of "Ct Oxa" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa

All present enzymatic activity of Cryo-Em Structure of "Ct Oxa" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa:
6.4.1.1;

The binding sites of Manganese atom in the Cryo-Em Structure of "Ct Oxa" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa (pdb code 7zyz). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Cryo-Em Structure of "Ct Oxa" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa, PDB code: 7zyz:

Manganese binding site 1 out of 1 in the Cryo-Em Structure of "Ct Oxa" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Cryo-Em Structure of "Ct Oxa" Conformation of Lactococcus Lactis Pyruvate Carboxylase with Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1201 b:34.8 occ:1.00 OQ2 A:KCX703 2.0 28.7 1.0 O A:HOH1320 2.0 31.2 1.0 NE2 A:HIS734 2.0 28.4 1.0 OD2 A:ASP534 2.1 26.8 1.0 NE2 A:HIS732 2.3 25.6 1.0 OQ1 A:KCX703 2.5 28.7 1.0 CX A:KCX703 2.6 28.7 1.0 CE1 A:HIS734 2.7 28.4 1.0 CG A:ASP534 3.1 26.8 1.0 CE1 A:HIS732 3.2 25.6 1.0 CD2 A:HIS734 3.2 28.4 1.0 OD1 A:ASP534 3.4 26.8 1.0 CD2 A:HIS732 3.4 25.6 1.0 O A:HOH1302 3.5 33.1 1.0 NZ A:KCX703 3.9 28.7 1.0 O1 A:OAA1202 3.9 35.3 1.0 ND1 A:HIS734 4.0 28.4 1.0 O3 A:OAA1202 4.0 35.3 1.0 NE2 A:GLN768 4.1 26.2 1.0 CG A:HIS734 4.2 28.4 1.0 NH2 A:ARG533 4.3 26.9 1.0 ND1 A:HIS732 4.3 25.6 1.0 O A:HOH1308 4.4 28.1 1.0 CG A:HIS732 4.5 25.6 1.0 CB A:ASP534 4.5 26.8 1.0 CE A:KCX703 4.7 28.7 1.0 CA A:MET705 4.8 33.5 1.0 O A:MET705 4.9 33.5 1.0 C1 A:OAA1202 4.9 35.3 1.0 C3 A:OAA1202 4.9 35.3 1.0

J.P.Lopez-Alonso, M.Lazaro, D.Gil-Carton, P.H.Choi, L.Tong, M.Valle. Cryoem Structural Exploration of Catalytically Active Enzyme Pyruvate Carboxylase. Nat Commun V. 13 6185 2022.
ISSN: ESSN 2041-1723
PubMed: 36261450
DOI: 10.1038/S41467-022-33987-2