Manganese in PDB 7xgc: Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan

The structure of Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan, PDB code: 7xgc was solved by Y.Guo, T.Hoshino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.54 / 1.60
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	66.177, 66.177, 125.727, 90, 90, 90
R / Rfree (%)	20.5 / 22.8

The binding sites of Manganese atom in the Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan (pdb code 7xgc). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan, PDB code: 7xgc:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn202 b:79.7 occ:1.00 O A:HOH380 2.8 38.1 1.0 OH A:TYR115 2.9 34.9 1.0 N A:VAL107 3.0 22.3 1.0 O A:VAL107 3.4 27.5 1.0 CA A:GLY106 3.5 27.2 1.0 C A:GLY106 3.7 25.3 1.0 O A:HOH315 3.7 37.0 1.0 CD2 A:HIS46 3.8 27.4 1.0 CZ A:TYR115 3.9 34.1 1.0 CA A:VAL107 4.0 23.3 1.0 C A:VAL107 4.0 25.7 1.0 O A:ILE105 4.0 27.6 1.0 CE1 A:TYR115 4.3 29.0 1.0 CB A:VAL107 4.4 24.1 1.0 NE2 A:HIS46 4.4 25.6 1.0 CG A:HIS46 4.5 24.3 1.0 NZ A:LYS119 4.6 52.4 1.0 N A:GLY106 4.6 24.4 1.0 O A:HOH347 4.6 30.1 1.0 C A:ILE105 4.8 28.9 1.0 CE2 A:TYR115 4.8 30.6 1.0 CB A:HIS46 4.9 27.3 1.0 O A:GLY106 4.9 28.1 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Influenza Polymerase Acidic Subunit N-Terminal Domain Crystallized By Ammonium Sulfate with Glycan within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn203 b:114.1 occ:1.00 OE1 A:GLU65 2.3 40.8 1.0 CD A:GLU65 3.4 37.0 1.0 CE1 A:HIS46 3.7 25.7 1.0 OE2 A:GLU65 3.8 44.7 1.0 O A:HOH336 3.9 38.8 1.0 OD1 A:ASP93 4.0 29.1 1.0 ND1 A:HIS46 4.0 26.3 1.0 O A:HOH348 4.3 34.1 1.0 O A:HOH316 4.3 42.2 1.0 NE2 A:HIS46 4.6 25.6 1.0 CG A:GLU65 4.7 34.4 1.0

Y.Guo, T.Hoshino. Influence of Glycan Agents on Protein Crystallization with Ammonium Sulfate Cryst.Growth Des. 2022.
ISSN: ESSN 1528-7505
DOI: 10.1021/ACS.CGD.2C00901