Manganese in PDB 7ulf: L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus

All present enzymatic activity of L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus:
6.3.2.31; 6.3.2.34;

The structure of L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus, PDB code: 7ulf was solved by G.Bashiri, C.J.Squire, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.04 / 1.61
Space group	P 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	68.71, 68.71, 92.45, 90, 90, 90
R / Rfree (%)	19.1 / 21.2

The binding sites of Manganese atom in the L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus (pdb code 7ulf). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus, PDB code: 7ulf:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn303 b:17.4 occ:1.00 O3G A:GTP302 2.1 17.7 1.0 OE1 A:GLU208 2.1 19.5 1.0 O2B A:GTP302 2.1 18.5 1.0 O A:HOH409 2.1 20.2 1.0 O2A A:GTP302 2.2 17.2 1.0 OG1 A:THR151 2.2 17.1 1.0 CD A:GLU208 3.0 20.9 1.0 PB A:GTP302 3.2 17.8 1.0 CB A:THR151 3.2 18.9 1.0 PG A:GTP302 3.3 20.0 1.0 OE2 A:GLU208 3.3 23.3 1.0 PA A:GTP302 3.4 19.3 1.0 O3B A:GTP302 3.4 17.5 1.0 O3A A:GTP302 3.5 17.1 1.0 O A:HOH430 3.9 25.3 1.0 O A:HOH448 4.0 21.2 1.0 O2G A:GTP302 4.0 19.3 1.0 CG2 A:THR151 4.0 19.5 1.0 OD1 A:ASN203 4.1 21.0 1.0 O A:HOH463 4.1 28.2 1.0 O A:GLY207 4.3 20.5 1.0 CG A:GLU208 4.3 20.8 1.0 CA A:GLU208 4.4 21.2 1.0 CA A:THR151 4.4 17.2 1.0 O1G A:GTP302 4.4 21.1 1.0 N A:THR151 4.5 17.8 1.0 O1A A:GTP302 4.5 19.2 1.0 O5' A:GTP302 4.5 19.7 1.0 C5' A:GTP302 4.5 18.4 1.0 O1B A:GTP302 4.6 17.5 1.0 CB A:GLU208 4.6 21.0 1.0 CG A:ASN203 4.9 19.6 1.0

Manganese binding site 2 out of 2 in the L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of L-Glutamate/Gtp Complex of F420-Gamma Glutamyl Ligase (Cofe) From Archaeoglobus Fulgidus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn304 b:23.0 occ:1.00 O2G A:GTP302 2.1 19.3 1.0 OD1 A:ASP150 2.1 23.3 1.0 O A:HOH469 2.2 31.8 1.0 OD2 A:ASP109 2.2 24.4 1.0 O A:HOH407 2.2 24.2 1.0 O A:HOH421 2.3 21.1 1.0 CG A:ASP150 3.1 23.8 1.0 CG A:ASP109 3.1 23.4 1.0 PG A:GTP302 3.3 20.0 1.0 OD2 A:ASP150 3.4 29.3 1.0 OD1 A:ASP109 3.4 24.1 1.0 O3B A:GTP302 3.6 17.5 1.0 O A:HOH415 3.7 31.1 1.0 O1G A:GTP302 3.8 21.1 1.0 O A:GLY107 4.0 23.6 1.0 O A:HOH427 4.0 28.4 1.0 OG A:SER40 4.2 26.2 1.0 O1B A:GTP302 4.3 17.5 1.0 PB A:GTP302 4.4 17.8 1.0 CB A:ASP150 4.5 21.5 1.0 CB A:ASP109 4.5 22.3 1.0 O3G A:GTP302 4.5 17.7 1.0 O A:HOH466 4.5 20.9 1.0 N A:THR151 4.6 17.8 1.0 O A:THR151 4.6 18.0 1.0 C A:GLY107 4.7 22.6 1.0 CA A:GLY107 4.7 21.9 1.0 CA A:ASP150 4.8 19.2 1.0 OG1 A:THR41 4.8 21.7 1.0 O2B A:GTP302 4.9 18.5 1.0

G.Bashiri, W.Bramley, E.Bulloch, S.Stutely, M.Middleditch, P.Young, M.Naqvi, P.Harris, E.N.Baker, C.J.Squire. A Universal Mechanism For Poly-Glutamylation To Be Published.