Manganese in PDB 7txg: Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P

The structure of Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P, PDB code: 7txg was solved by C.Abad-Zapatero, A.I.Selezneva, L.N.M.Harding, F.Movahedzadeh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.94 / 1.90
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	64.176, 76.226, 77.924, 68.02, 68.22, 76.65
R / Rfree (%)	18.7 / 22.1

The binding sites of Manganese atom in the Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P (pdb code 7txg). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P, PDB code: 7txg:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:75.8 occ:1.00 OD1 A:ASP84 2.2 42.0 1.0 O A:HOH565 2.4 45.6 1.0 O A:LEU86 2.5 37.1 1.0 CG A:ASP84 3.3 40.7 1.0 C A:LEU86 3.4 37.8 1.0 OD2 A:ASP84 3.8 39.1 1.0 N A:GLU87 4.1 34.1 1.0 OE1 A:GLU57 4.2 53.7 1.0 N A:LEU86 4.2 33.5 1.0 CA A:GLU87 4.2 37.8 1.0 CA A:LEU86 4.4 34.7 1.0 O A:HOH592 4.6 46.6 1.0 CB A:ASP84 4.6 39.6 1.0 CG2 A:THR89 4.6 42.8 1.0 CA A:ASP84 4.8 37.0 1.0 OD1 A:ASP33 4.8 57.0 1.0 OE1 A:GLU214 4.9 48.2 1.0 CD A:PRO85 4.9 42.5 1.0 N A:PRO85 4.9 38.3 1.0 C A:ASP84 5.0 40.2 1.0 N A:GLY88 5.0 37.1 1.0

Manganese binding site 2 out of 4 in the Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn406 b:78.2 occ:1.00 OD1 B:ASP84 2.2 47.4 1.0 O B:HOH567 2.4 54.7 1.0 O B:LEU86 2.5 45.3 1.0 CG B:ASP84 3.3 47.5 1.0 C B:LEU86 3.4 44.6 1.0 O B:HOH580 3.5 47.0 1.0 OD2 B:ASP84 3.6 46.4 1.0 O B:HOH506 3.8 46.4 1.0 N B:GLU87 4.2 38.8 1.0 N B:LEU86 4.2 40.6 1.0 CA B:GLU87 4.3 40.9 1.0 CA B:LEU86 4.4 41.3 1.0 CB B:ASP84 4.6 45.5 1.0 CG2 B:THR89 4.6 49.3 1.0 OD1 B:ASP33 4.7 54.7 1.0 OE1 B:GLU57 4.7 61.0 1.0 O B:HOH516 4.7 47.7 1.0 CA B:ASP84 4.8 44.6 1.0 CD B:PRO85 4.9 48.6 1.0 N B:GLY88 4.9 42.5 1.0 N B:PRO85 4.9 45.8 1.0 CB B:LEU86 5.0 42.8 1.0 C B:ASP84 5.0 45.0 1.0

Manganese binding site 3 out of 4 in the Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn703 b:52.5 occ:1.00 O C:HOH989 2.2 35.2 1.0 OD1 C:ASP84 2.2 31.6 1.0 O C:HOH939 2.3 35.8 1.0 O C:HOH969 2.5 40.8 1.0 O C:LEU86 2.5 24.3 1.0 O C:HOH802 3.1 35.7 1.0 CG C:ASP84 3.3 33.4 1.0 C C:LEU86 3.5 26.1 1.0 O C:HOH806 3.7 42.6 1.0 OD2 C:ASP84 3.7 31.7 1.0 O C:HOH926 4.0 28.5 1.0 N C:GLU87 4.1 25.1 1.0 CA C:GLU87 4.2 29.3 1.0 N C:LEU86 4.2 26.8 1.0 O C:HOH865 4.3 45.0 1.0 O C:HOH816 4.4 37.0 1.0 CA C:LEU86 4.4 26.6 1.0 CG2 C:THR89 4.6 27.6 1.0 CB C:ASP84 4.6 28.7 1.0 OE1 C:GLU57 4.7 40.4 1.0 N C:GLY88 4.8 23.2 1.0 OE2 C:GLU214 4.8 32.0 1.0 CA C:ASP84 4.9 29.0 1.0 CB C:LEU86 5.0 25.9 1.0 OD1 C:ASP33 5.0 42.3 1.0

Manganese binding site 4 out of 4 in the Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Structure of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis with Native Mn++ Divalent Cation and Partially Occupied Product F6P within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn607 b:90.8 occ:1.00 OD1 D:ASP84 2.2 49.0 1.0 OE2 D:GLU57 2.2 102.5 1.0 OD2 D:ASP84 2.3 56.0 1.0 CG D:ASP84 2.5 45.0 1.0 O D:LEU86 3.0 31.0 1.0 CD D:GLU57 3.5 110.7 1.0 O D:HOH861 3.7 49.4 1.0 C D:LEU86 3.8 36.5 1.0 CB D:ASP84 3.9 41.2 1.0 OE1 D:GLU57 4.2 111.8 1.0 CA D:GLU87 4.2 39.0 1.0 N D:GLU87 4.3 36.2 1.0 CA D:ASP84 4.5 42.4 1.0 CG D:GLU57 4.5 108.8 1.0 OE2 D:GLU214 4.6 36.2 1.0 N D:LEU86 4.6 36.9 1.0 CD D:PRO85 4.8 46.1 1.0 CA D:LEU86 4.9 37.6 1.0 C D:ASP84 5.0 39.3 1.0

A.I.Selezneva, L.N.M.Harding, H.J.Gutka, F.Movahedzadeh, C.Abad-Zapatero. New Structures of Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis Provide A Framework For A Novel Catalytic Mechanism For the Entire Class To Be Published.