Manganese in PDB 7qjj: X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A

The structure of X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A, PDB code: 7qjj was solved by K.Ramanadane, M.S.Straub, R.Dutzler, C.Manatschal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	12.00 / 4.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	92.9, 115.93, 148.66, 90, 107.59, 90
R / Rfree (%)	28.4 / 32.7

The binding sites of Manganese atom in the X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A (pdb code 7qjj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A, PDB code: 7qjj:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:183.0 occ:1.00 O A:THR224 3.2 183.7 1.0 OD1 A:ASP55 3.2 241.9 1.0 O A:GLY53 3.7 315.7 1.0 CG A:ASP55 4.2 257.4 1.0 C A:THR224 4.3 196.4 1.0 OD1 A:ASN54 4.4 284.9 1.0 N A:ASP55 4.5 288.0 1.0 OG1 A:THR224 4.5 192.7 1.0 OD2 A:ASP55 4.5 246.0 1.0 CA A:ASN54 4.5 322.3 1.0 CA A:GLY58 4.6 252.1 1.0 C A:GLY53 4.6 361.4 1.0 CA A:THR225 4.7 226.2 1.0 CE A:MET230 4.8 234.2 1.0 N A:GLY58 5.0 245.3 1.0

Manganese binding site 2 out of 2 in the X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of X-Ray Structure of A MN2+ Soak of Elenrmt in Complex with Two Nanobodies at 4.6A within 5.0Å range: probe atom residue distance (Å) B Occ E:Mn501 b:200.9 occ:1.00 O E:THR224 3.2 178.6 1.0 OD1 E:ASP55 3.2 265.8 1.0 O E:GLY53 3.6 310.6 1.0 CG E:ASP55 4.2 267.1 1.0 C E:THR224 4.4 178.4 1.0 OD1 E:ASN54 4.4 279.4 1.0 N E:ASP55 4.5 314.9 1.0 CA E:ASN54 4.5 335.9 1.0 OD2 E:ASP55 4.5 251.1 1.0 C E:GLY53 4.6 364.8 1.0 OG1 E:THR224 4.6 196.1 1.0 CA E:GLY58 4.6 289.3 1.0 CA E:THR225 4.8 183.2 1.0 CE E:MET230 4.8 224.0 1.0 N E:ASN54 5.0 358.6 1.0 N E:GLY58 5.0 288.9 1.0

K.Ramanadane, M.S.Straub, R.Dutzler, C.Manatschal. Structural and Functional Properties of A Magnesium Transporter of the SLC11/Nramp Family. Elife V. 11 2022.
ISSN: ESSN 2050-084X
PubMed: 35001872
DOI: 10.7554/ELIFE.74589