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Manganese in PDB 7d6g: Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5

Enzymatic activity of Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5

All present enzymatic activity of Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5:
1.5.1.3;

Protein crystallography data

The structure of Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5, PDB code: 7d6g was solved by Q.Wan, C.Dealwis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 1.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 34.102, 45.595, 99.113, 90, 90, 90
R / Rfree (%) 17.7 / 21.9

Manganese Binding Sites:

The binding sites of Manganese atom in the Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5 (pdb code 7d6g). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5, PDB code: 7d6g:

Manganese binding site 1 out of 1 in 7d6g

Go back to Manganese Binding Sites List in 7d6g
Manganese binding site 1 out of 1 in the Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Neutron Crystal Structure of E.Coli Dihydrofolate Reductase Complexed with Folate and Nadp+ at PH4.5 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn201

b:86.8
occ:1.00
OD1 A:ASP87 2.6 52.4 1.0
CG A:ASP87 3.6 57.7 1.0
OD2 A:ASP87 3.9 66.2 1.0
D1 A:DOD336 4.8 34.6 1.0
HA A:ASP87 4.8 37.5 1.0
CB A:ASP87 5.0 29.9 1.0

Reference:

Q.Wan, B.C.Bennett, T.Wymore, Z.Li, M.A.Wilson, C.L.Brooks Iii, P.Langan, A.Kovalevsky, C.G.Dealwis. Capturing the Catalytic Proton of Dihydrofolate Reductase: Implications For General Acid-Base Catalysis Acs Catalysis V. 11 5873 2021.
ISSN: ESSN 2155-5435
DOI: 10.1021/ACSCATAL.1C00417
Page generated: Sun Oct 6 08:24:31 2024

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