Manganese in PDB 6yvc: Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa

The structure of Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa, PDB code: 6yvc was solved by F.Altegoer, G.Bange, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.78 / 1.85
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	85.33, 85.38, 122.17, 90, 102.06, 90
R / Rfree (%)	19.1 / 22.2

The binding sites of Manganese atom in the Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa (pdb code 6yvc). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa, PDB code: 6yvc:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:24.1 occ:0.48 NE2 A:HIS70 2.1 27.4 1.0 O A:HOH314 2.2 32.9 1.0 NE2 A:HIS40 2.2 20.1 1.0 OD1 A:ASP131 2.2 23.3 1.0 O A:HOH379 2.3 34.4 1.0 OD2 A:ASP131 2.8 24.3 1.0 CG A:ASP131 2.8 22.0 1.0 CE1 A:HIS70 3.0 26.0 1.0 CE1 A:HIS40 3.1 19.3 1.0 CD2 A:HIS70 3.1 26.9 1.0 CD2 A:HIS40 3.1 20.1 1.0 O A:HOH420 3.3 46.1 1.0 OH A:TYR37 3.6 37.3 1.0 O A:HOH434 4.0 35.0 1.0 ND1 A:HIS70 4.2 24.6 1.0 ND1 A:HIS40 4.2 18.6 1.0 ND2 A:ASN135 4.2 17.9 1.0 CG A:HIS70 4.2 23.9 1.0 CG A:HIS40 4.2 19.4 1.0 CB A:ASP131 4.3 17.5 1.0 CG2 A:VAL44 4.7 13.2 1.0 CZ A:TYR37 4.8 32.1 1.0 O A:HOH433 4.9 34.8 1.0 CA A:ASP131 4.9 14.2 1.0

Manganese binding site 2 out of 4 in the Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn201 b:21.3 occ:0.50 NE2 B:HIS70 2.1 28.9 1.0 OD1 B:ASP131 2.2 22.7 1.0 NE2 B:HIS40 2.2 22.2 1.0 O B:HOH326 2.3 34.4 1.0 O B:HOH368 2.4 42.5 1.0 O B:HOH308 2.8 44.5 1.0 CG B:ASP131 2.8 21.5 1.0 OD2 B:ASP131 2.9 22.0 1.0 CE1 B:HIS70 3.0 30.1 1.0 CE1 B:HIS40 3.0 19.7 1.0 CD2 B:HIS70 3.1 30.7 1.0 CD2 B:HIS40 3.2 19.9 1.0 OH B:TYR37 3.7 35.6 1.0 ND1 B:HIS40 4.1 18.9 1.0 ND1 B:HIS70 4.1 29.7 1.0 ND2 B:ASN135 4.2 20.8 1.0 CG B:HIS70 4.3 25.6 1.0 CB B:ASP131 4.3 17.1 1.0 CG B:HIS40 4.3 18.3 1.0 O B:HOH447 4.4 41.1 1.0 O B:HOH470 4.5 42.2 1.0 CG2 B:VAL44 4.6 12.2 1.0 CZ B:TYR37 4.9 27.2 1.0 O B:HOH417 4.9 40.3 1.0 CA B:ASP131 4.9 13.9 1.0

Manganese binding site 3 out of 4 in the Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn201 b:28.5 occ:0.48 NE2 C:HIS70 2.0 32.3 1.0 O C:HOH307 2.2 32.5 1.0 NE2 C:HIS40 2.2 25.3 1.0 OD1 C:ASP131 2.4 26.1 1.0 O C:HOH444 2.6 36.4 1.0 CE1 C:HIS70 2.8 32.0 1.0 CG C:ASP131 3.0 25.7 1.0 OD2 C:ASP131 3.0 25.4 1.0 CD2 C:HIS70 3.1 32.2 1.0 CD2 C:HIS40 3.1 22.5 1.0 CE1 C:HIS40 3.1 23.7 1.0 OH C:TYR37 3.5 38.5 1.0 ND1 C:HIS70 4.0 30.0 1.0 CG C:HIS70 4.2 29.4 1.0 ND1 C:HIS40 4.2 22.6 1.0 CG C:HIS40 4.3 24.9 1.0 ND2 C:ASN135 4.3 21.9 1.0 CB C:ASP131 4.3 18.4 1.0 O C:HOH456 4.5 51.7 1.0 CG2 C:VAL44 4.7 12.3 1.0 O C:HOH424 4.7 37.1 1.0 CZ C:TYR37 4.7 33.9 1.0 CB C:ASP71 5.0 25.1 1.0

Manganese binding site 4 out of 4 in the Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of the Small Alarmone Hydrolase (Sah) of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn201 b:21.7 occ:0.44 O D:HOH405 2.0 29.6 1.0 NE2 D:HIS70 2.1 23.9 1.0 NE2 D:HIS40 2.2 26.5 1.0 OD1 D:ASP131 2.3 24.4 1.0 CG D:ASP131 2.9 25.1 1.0 OD2 D:ASP131 2.9 26.2 1.0 O D:HOH341 2.9 42.6 1.0 CE1 D:HIS70 3.0 22.0 1.0 CE1 D:HIS40 3.1 25.0 1.0 CD2 D:HIS70 3.1 23.9 1.0 CD2 D:HIS40 3.1 24.1 1.0 OH D:TYR37 3.7 38.7 1.0 ND1 D:HIS70 4.1 23.5 1.0 ND1 D:HIS40 4.2 25.0 1.0 CG D:HIS70 4.2 21.0 1.0 CG D:HIS40 4.2 23.4 1.0 ND2 D:ASN135 4.3 22.6 1.0 CB D:ASP131 4.3 19.6 1.0 O D:HOH454 4.4 35.3 1.0 O D:HOH392 4.4 30.9 1.0 CG2 D:VAL44 4.6 15.2 1.0 O D:HOH411 4.8 31.5 1.0 CZ D:TYR37 4.9 32.9 1.0 CA D:ASP131 5.0 18.5 1.0

W.Steinchen, M.Valentini, K.Glatzel, M.Majkini, F.Altegoer, M.Lechner, A.Filloux, G.Bange. Characterization of A Bacterial Small (P)Ppgpp Hydrolase Suggests A Role in Pseudomonas Aeruginosa Biofilm Formation To Be Published.