Manganese in PDB 6wxx: Crystal Structure of CA4-Activated CARD1

The structure of Crystal Structure of CA4-Activated CARD1, PDB code: 6wxx was solved by J.Rostol, W.Xie, D.J.Patel, L.Marraffini, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.88 / 3.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	85.064, 121.216, 103.708, 90, 113.06, 90
R / Rfree (%)	20.4 / 23.7

The binding sites of Manganese atom in the Crystal Structure of CA4-Activated CARD1 (pdb code 6wxx). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Crystal Structure of CA4-Activated CARD1, PDB code: 6wxx:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Crystal Structure of CA4-Activated CARD1


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of CA4-Activated CARD1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn901 b:123.9 occ:1.00 O B:CYS309 2.8 78.7 1.0 OD2 B:ASP294 2.8 84.1 1.0 NZ B:LYS310 3.4 84.4 1.0 OE1 B:GLU308 3.4 83.5 1.0 CG B:ASP294 3.6 76.8 1.0 OD1 B:ASP294 3.7 80.0 1.0 CE B:LYS310 3.9 76.8 1.0 C B:CYS309 3.9 62.6 1.0 OE2 B:GLU259 4.1 96.7 1.0 CD B:LYS310 4.1 65.2 1.0 CD B:GLU308 4.5 89.0 1.0 N B:CYS309 4.6 69.6 1.0 CA B:CYS309 4.7 59.1 1.0 N B:LYS310 4.9 50.1 1.0 CB B:ASP294 5.0 63.0 1.0 CB B:CYS309 5.0 56.9 1.0 CA B:LYS310 5.0 49.2 1.0

Manganese binding site 2 out of 4 in the Crystal Structure of CA4-Activated CARD1


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of CA4-Activated CARD1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn901 b:124.8 occ:1.00 O A:CYS309 3.2 67.4 1.0 OE2 A:GLU259 3.3 97.4 1.0 OD1 A:ASP294 3.4 89.5 1.0 CG A:ASP294 4.0 82.2 1.0 OD2 A:ASP294 4.1 84.8 1.0 NZ A:LYS310 4.3 73.1 1.0 C A:CYS309 4.4 64.0 1.0 CD A:GLU259 4.5 82.2 1.0 OE2 A:GLU308 4.5 83.1 1.0 CD A:LYS310 4.8 65.6 1.0 CE A:LYS310 5.0 76.7 1.0

Manganese binding site 3 out of 4 in the Crystal Structure of CA4-Activated CARD1


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of CA4-Activated CARD1 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn901 b:110.1 occ:1.00 O D:CYS309 2.8 75.4 1.0 OD2 D:ASP294 3.0 87.3 1.0 CG D:ASP294 3.8 73.3 1.0 NZ D:LYS310 3.8 56.3 1.0 OE1 D:GLU259 3.9 82.1 1.0 OD1 D:ASP294 4.0 79.1 1.0 C D:CYS309 4.0 62.4 1.0 OE2 D:GLU308 4.1 91.1 1.0 CE D:LYS310 4.4 56.1 1.0 CD D:GLU259 4.4 77.1 1.0 CD D:LYS310 4.4 62.7 1.0 CG D:GLU259 4.8 70.7 1.0 CA D:CYS309 4.9 56.9 1.0 N D:CYS309 4.9 62.2 1.0 N D:LYS310 5.0 59.4 1.0 CB D:CYS309 5.0 59.1 1.0 CA D:LYS310 5.0 57.4 1.0

Manganese binding site 4 out of 4 in the Crystal Structure of CA4-Activated CARD1


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of CA4-Activated CARD1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn901 b:135.2 occ:1.00 OE2 C:GLU259 3.1 97.6 1.0 O C:CYS309 3.7 81.0 1.0 NZ C:LYS310 3.8 88.4 1.0 OD2 C:ASP294 3.9 90.8 1.0 CD C:GLU259 4.3 91.8 1.0 OD1 C:ASP294 4.5 99.2 1.0 CG C:ASP294 4.5 91.1 1.0 CE C:LYS310 4.7 90.1 1.0 CD C:LYS310 4.7 94.3 1.0 OE1 C:GLU308 4.9 77.2 1.0 C C:CYS309 4.9 75.9 1.0

J.Rostol, W.Xie, D.J.Patel, L.Marraffini. Crystal Structure of CA4-Activated CARD1 To Be Published.