Manganese in PDB 6wb5: Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp

The structure of Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp, PDB code: 6wb5 was solved by P.D.Jeffrey, J.N.Balaich, M.A.Estrella, M.S.Donia, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.33 / 3.10
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	86.551, 86.551, 164.334, 90, 90, 120
R / Rfree (%)	16.3 / 24.9

The binding sites of Manganese atom in the Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp (pdb code 6wb5). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp, PDB code: 6wb5:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn303 b:86.9 occ:1.00 O3G A:ANP302 2.0 92.0 1.0 ND1 A:HIS162 2.1 42.3 1.0 O2G A:ANP302 2.1 219.5 1.0 O2B A:ANP302 2.4 156.3 1.0 PG A:ANP302 2.5 260.5 1.0 CE1 A:HIS162 2.8 40.1 1.0 CG A:HIS162 3.2 42.4 1.0 PB A:ANP302 3.6 187.5 1.0 N3B A:ANP302 3.7 52.8 1.0 O1G A:ANP302 3.7 258.5 1.0 CB A:HIS162 3.8 42.9 1.0 CA A:HIS162 3.8 52.1 1.0 OD2 A:ASP160 3.9 51.3 1.0 OD1 A:ASP160 3.9 42.6 1.0 NE2 A:HIS162 4.0 43.6 1.0 CD2 A:HIS162 4.2 43.4 1.0 CG A:ASP160 4.2 44.8 1.0 O6B C:AC13 4.5 65.6 1.0 O1B A:ANP302 4.6 188.7 1.0 CE A:MET210 4.6 120.2 1.0 O3A A:ANP302 4.7 133.4 1.0 N A:HIS162 4.7 57.8 1.0 CA A:GLY246 4.8 55.4 1.0 C A:HIS162 4.9 51.8 1.0 CB A:SER184 5.0 40.1 1.0

Manganese binding site 2 out of 2 in the Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Microbiome-Derived Acarbose Kinase MAK1 As A Complex with Acarbose and Amp-Pnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn303 b:61.9 occ:1.00 O2B B:ANP302 2.0 134.0 1.0 O2G B:ANP302 2.1 209.0 1.0 ND1 B:HIS162 2.2 51.6 1.0 CE1 B:HIS162 2.8 50.9 1.0 PB B:ANP302 3.3 65.5 1.0 CG B:HIS162 3.4 36.0 1.0 PG B:ANP302 3.6 282.1 1.0 O1B B:ANP302 3.7 80.7 1.0 CB B:HIS162 3.9 29.4 1.0 CA B:HIS162 4.0 42.3 1.0 NE2 B:HIS162 4.0 40.1 1.0 N3B B:ANP302 4.0 117.1 1.0 OD2 B:ASP160 4.1 72.9 1.0 OD1 B:ASP160 4.2 72.9 1.0 CD2 B:HIS162 4.3 29.4 1.0 O1G B:ANP302 4.3 281.3 1.0 O3A B:ANP302 4.5 96.2 1.0 CG B:ASP160 4.5 69.9 1.0 O3G B:ANP302 4.6 146.7 1.0 CE B:MET210 4.7 86.7 1.0 CA B:GLY246 4.8 49.9 1.0 CB B:SER184 4.9 57.5 1.0 O6B D:AC13 4.9 61.9 1.0 N B:HIS162 4.9 50.3 1.0

J.N.Balaich, M.A.Estrella, P.D.Jeffrey, A.Biswas, A.Korennykh, M.S.Donia. Acarbose Sugar Kinases Encoded By the Human Microbiome Mediates Microbial Competition For Complex Carbohydrates To Be Published.