Manganese in PDB 6vf6: Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Enzymatic activity of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
All present enzymatic activity of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min):
2.7.7.7;
Protein crystallography data
The structure of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min), PDB code: 6vf6
was solved by
J.A.Jamsen,
S.H.Wilson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
33.44 /
1.69
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
60.263,
62.333,
118.847,
90,
90,
90
|
R / Rfree (%)
|
16.6 /
18.9
|
Other elements in 6vf6:
The structure of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) also contains other interesting chemical elements:
Manganese Binding Sites:
The binding sites of Manganese atom in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
(pdb code 6vf6). This binding sites where shown within
5.0 Angstroms radius around Manganese atom.
In total 8 binding sites of Manganese where determined in the
Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min), PDB code: 6vf6:
Jump to Manganese binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Manganese binding site 1 out
of 8 in 6vf6
Go back to
Manganese Binding Sites List in 6vf6
Manganese binding site 1 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 1 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn504
b:18.5
occ:1.00
|
OP1
|
P:8GM5
|
2.0
|
21.8
|
1.0
|
OD2
|
A:ASP418
|
2.1
|
17.8
|
1.0
|
OD1
|
A:ASP332
|
2.1
|
21.2
|
1.0
|
O
|
P:HOH105
|
2.2
|
18.6
|
1.0
|
O
|
A:HOH752
|
2.4
|
34.5
|
1.0
|
OD2
|
A:ASP330
|
2.4
|
26.2
|
1.0
|
CG
|
A:ASP418
|
3.0
|
17.8
|
1.0
|
CG
|
A:ASP332
|
3.1
|
18.3
|
1.0
|
P
|
P:8GM5
|
3.2
|
20.5
|
1.0
|
CG
|
A:ASP330
|
3.2
|
27.4
|
1.0
|
O3'
|
P:DA4
|
3.3
|
20.4
|
1.0
|
MN
|
A:MN505
|
3.3
|
18.4
|
1.0
|
OD2
|
A:ASP332
|
3.5
|
18.2
|
1.0
|
CB
|
A:ASP418
|
3.8
|
17.5
|
1.0
|
CB
|
A:ASP330
|
3.8
|
21.7
|
1.0
|
OD1
|
A:ASP418
|
3.9
|
16.3
|
1.0
|
OP2
|
P:8GM5
|
4.0
|
20.3
|
1.0
|
OD1
|
A:ASP330
|
4.1
|
31.1
|
1.0
|
C5'
|
P:DA4
|
4.2
|
19.4
|
1.0
|
O2
|
A:GOA512
|
4.3
|
23.1
|
1.0
|
C3'
|
P:DA4
|
4.4
|
20.3
|
1.0
|
NH2
|
A:ARG416
|
4.4
|
16.3
|
1.0
|
O
|
P:HOH102
|
4.4
|
35.7
|
1.0
|
CB
|
A:ASP332
|
4.4
|
15.2
|
1.0
|
C4'
|
P:DA4
|
4.4
|
19.9
|
1.0
|
O5'
|
P:8GM5
|
4.5
|
21.1
|
1.0
|
O
|
A:VAL331
|
4.5
|
17.5
|
1.0
|
OP1
|
P:DA4
|
4.6
|
18.8
|
1.0
|
N
|
A:ASP418
|
4.7
|
15.2
|
1.0
|
C
|
A:VAL331
|
4.8
|
16.4
|
1.0
|
CA
|
A:ASP418
|
4.9
|
16.3
|
1.0
|
CA
|
A:ASP332
|
4.9
|
15.4
|
1.0
|
CZ3
|
A:TRP434
|
4.9
|
16.5
|
1.0
|
C5'
|
P:8GM5
|
4.9
|
21.4
|
1.0
|
O5'
|
P:DA4
|
5.0
|
19.4
|
1.0
|
N
|
A:ASP332
|
5.0
|
15.7
|
1.0
|
N
|
A:VAL331
|
5.0
|
17.1
|
1.0
|
|
Manganese binding site 2 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 2 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 2 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn505
b:18.4
occ:1.00
|
OD2
|
A:ASP332
|
2.1
|
18.2
|
1.0
|
O12
|
A:GOA512
|
2.1
|
19.2
|
1.0
|
O2
|
A:GOA512
|
2.1
|
23.1
|
1.0
|
OD2
|
A:ASP330
|
2.1
|
26.2
|
1.0
|
O
|
A:HOH691
|
2.2
|
18.7
|
1.0
|
OP1
|
P:8GM5
|
2.3
|
21.8
|
1.0
|
C1
|
A:GOA512
|
2.9
|
22.7
|
1.0
|
CG
|
A:ASP330
|
2.9
|
27.4
|
1.0
|
C2
|
A:GOA512
|
3.0
|
23.3
|
1.0
|
CG
|
A:ASP332
|
3.1
|
18.3
|
1.0
|
OD1
|
A:ASP330
|
3.2
|
31.1
|
1.0
|
MN
|
A:MN504
|
3.3
|
18.5
|
1.0
|
OD1
|
A:ASP332
|
3.4
|
21.2
|
1.0
|
P
|
P:8GM5
|
3.5
|
20.5
|
1.0
|
O5'
|
P:8GM5
|
3.8
|
21.1
|
1.0
|
O
|
A:HOH793
|
4.1
|
27.1
|
1.0
|
O11
|
A:GOA512
|
4.1
|
23.4
|
1.0
|
C5'
|
P:8GM5
|
4.2
|
21.4
|
1.0
|
O
|
P:HOH102
|
4.2
|
35.7
|
1.0
|
OP2
|
P:8GM5
|
4.2
|
20.3
|
1.0
|
O
|
A:ASP330
|
4.2
|
18.4
|
1.0
|
O
|
P:HOH105
|
4.3
|
18.6
|
1.0
|
O
|
A:HOH637
|
4.3
|
17.3
|
1.0
|
N
|
A:GLY320
|
4.4
|
17.4
|
1.0
|
CA
|
A:GLY319
|
4.4
|
16.5
|
1.0
|
CB
|
A:ASP330
|
4.4
|
21.7
|
1.0
|
O
|
A:HOH742
|
4.4
|
40.0
|
1.0
|
CB
|
A:ASP332
|
4.4
|
15.2
|
1.0
|
C
|
A:ASP330
|
4.5
|
18.1
|
1.0
|
O3'
|
P:DA4
|
4.7
|
20.4
|
1.0
|
O
|
A:HOH752
|
4.8
|
34.5
|
1.0
|
N
|
A:ASP332
|
4.9
|
15.7
|
1.0
|
C
|
A:GLY319
|
5.0
|
17.5
|
1.0
|
CA
|
A:ASP330
|
5.0
|
17.7
|
1.0
|
|
Manganese binding site 3 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 3 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 3 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn506
b:72.5
occ:0.80
|
NE2
|
A:HIS152
|
2.3
|
64.5
|
1.0
|
O
|
A:HOH784
|
2.3
|
44.3
|
0.7
|
O
|
A:HOH616
|
2.8
|
47.2
|
0.8
|
CD2
|
A:HIS152
|
3.2
|
63.7
|
1.0
|
CE1
|
A:HIS152
|
3.2
|
66.3
|
1.0
|
ND1
|
A:HIS152
|
4.4
|
65.2
|
1.0
|
O
|
A:HOH649
|
4.4
|
31.7
|
1.0
|
CG
|
A:HIS152
|
4.4
|
60.5
|
1.0
|
O
|
A:PRO190
|
4.4
|
36.7
|
1.0
|
O
|
A:HOH803
|
4.5
|
50.4
|
1.0
|
O
|
A:THR150
|
4.6
|
29.9
|
1.0
|
|
Manganese binding site 4 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 4 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 4 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn507
b:34.6
occ:0.48
|
O
|
D:HOH214
|
1.9
|
43.8
|
0.9
|
O
|
D:HOH213
|
2.1
|
42.5
|
1.0
|
OP3
|
D:DG1
|
2.1
|
36.8
|
1.0
|
O
|
D:HOH215
|
2.3
|
59.1
|
0.8
|
ND1
|
A:HIS208
|
2.5
|
33.4
|
1.0
|
CE1
|
A:HIS208
|
3.4
|
34.9
|
1.0
|
P
|
D:DG1
|
3.4
|
31.3
|
1.0
|
CG
|
A:HIS208
|
3.4
|
30.6
|
1.0
|
O
|
D:HOH212
|
3.7
|
47.6
|
1.0
|
CB
|
A:HIS208
|
3.7
|
27.4
|
1.0
|
OP1
|
D:DG1
|
3.7
|
28.4
|
1.0
|
OP2
|
D:DG1
|
4.1
|
30.7
|
1.0
|
NE2
|
A:HIS208
|
4.4
|
33.9
|
1.0
|
CD2
|
A:HIS208
|
4.5
|
33.1
|
1.0
|
O5'
|
D:DG1
|
4.5
|
28.3
|
1.0
|
CA
|
A:HIS208
|
4.7
|
26.8
|
1.0
|
C5'
|
D:DG1
|
4.8
|
28.0
|
1.0
|
|
Manganese binding site 5 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 5 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 5 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn508
b:60.9
occ:0.80
|
NE2
|
A:HIS219
|
2.1
|
73.4
|
1.0
|
O
|
A:HOH746
|
2.2
|
45.9
|
1.0
|
CD2
|
A:HIS219
|
2.9
|
65.9
|
1.0
|
CE1
|
A:HIS219
|
3.1
|
69.6
|
1.0
|
CG
|
A:HIS219
|
4.1
|
61.9
|
1.0
|
ND1
|
A:HIS219
|
4.2
|
68.2
|
1.0
|
CB
|
A:GLU218
|
4.6
|
40.7
|
1.0
|
CG
|
A:GLU215
|
5.0
|
34.6
|
1.0
|
|
Manganese binding site 6 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 6 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 6 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn509
b:21.3
occ:0.74
|
O
|
T:HOH209
|
2.0
|
25.9
|
1.0
|
O
|
A:HOH792
|
2.1
|
28.9
|
1.0
|
O
|
A:HOH727
|
2.2
|
28.3
|
1.0
|
OE2
|
A:GLU386
|
2.2
|
28.6
|
1.0
|
OE1
|
A:GLU386
|
2.2
|
24.5
|
1.0
|
NE2
|
A:HIS459
|
2.3
|
32.1
|
1.0
|
CD
|
A:GLU386
|
2.5
|
27.1
|
1.0
|
CD2
|
A:HIS459
|
3.1
|
30.0
|
1.0
|
CE1
|
A:HIS459
|
3.4
|
34.9
|
1.0
|
CG
|
A:GLU386
|
4.1
|
30.1
|
1.0
|
OP1
|
T:DC8
|
4.3
|
24.0
|
1.0
|
O
|
A:HOH700
|
4.3
|
26.5
|
1.0
|
CG
|
A:HIS459
|
4.3
|
26.1
|
1.0
|
O
|
A:HOH697
|
4.4
|
22.7
|
1.0
|
O
|
A:HOH800
|
4.4
|
43.8
|
1.0
|
ND1
|
A:HIS459
|
4.4
|
30.2
|
1.0
|
OE1
|
A:GLN426
|
4.4
|
22.5
|
1.0
|
C5'
|
T:DC8
|
4.5
|
17.6
|
1.0
|
CB
|
A:GLU386
|
4.9
|
26.9
|
1.0
|
O
|
T:HOH227
|
5.0
|
39.4
|
1.0
|
|
Manganese binding site 7 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 7 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 7 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
T:Mn101
b:56.5
occ:0.62
|
O
|
T:HOH233
|
2.1
|
62.3
|
0.9
|
N7
|
T:DG2
|
2.3
|
28.4
|
1.0
|
O
|
T:HOH219
|
2.4
|
48.5
|
1.0
|
O
|
T:HOH205
|
2.6
|
35.9
|
0.8
|
C8
|
T:DG2
|
3.1
|
26.8
|
1.0
|
C5
|
T:DG2
|
3.3
|
24.9
|
1.0
|
O6
|
T:DG2
|
3.8
|
27.5
|
1.0
|
C6
|
T:DG2
|
3.9
|
25.8
|
1.0
|
O
|
T:HOH207
|
4.3
|
40.0
|
1.0
|
N9
|
T:DG2
|
4.3
|
25.6
|
1.0
|
O
|
D:HOH211
|
4.3
|
67.2
|
1.0
|
C4
|
T:DG2
|
4.4
|
25.3
|
1.0
|
O6
|
T:DG3
|
4.5
|
25.1
|
1.0
|
C6
|
T:DC1
|
4.9
|
25.0
|
1.0
|
C2'
|
T:DC1
|
4.9
|
37.4
|
1.0
|
|
Manganese binding site 8 out
of 8 in 6vf6
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Manganese Binding Sites List in 6vf6
Manganese binding site 8 out
of 8 in the Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min)
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 8 of Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mn101
b:64.0
occ:0.53
|
O
|
D:HOH211
|
2.2
|
67.2
|
1.0
|
N7
|
D:DG1
|
2.2
|
25.0
|
1.0
|
O
|
D:HOH205
|
2.6
|
39.1
|
1.0
|
C8
|
D:DG1
|
3.1
|
23.2
|
1.0
|
C5
|
D:DG1
|
3.4
|
23.1
|
1.0
|
O6
|
D:DG1
|
3.8
|
23.8
|
1.0
|
C6
|
D:DG1
|
4.0
|
23.1
|
1.0
|
N4
|
D:DC2
|
4.3
|
28.4
|
1.0
|
O
|
D:HOH204
|
4.3
|
53.6
|
1.0
|
N9
|
D:DG1
|
4.4
|
22.8
|
1.0
|
C4
|
D:DG1
|
4.5
|
22.1
|
1.0
|
O
|
T:HOH219
|
4.6
|
48.5
|
1.0
|
OP2
|
D:DG1
|
4.6
|
30.7
|
1.0
|
CG
|
A:ARG175
|
4.8
|
28.6
|
1.0
|
O
|
A:HOH791
|
4.9
|
33.6
|
1.0
|
C4
|
D:DC2
|
4.9
|
25.7
|
1.0
|
|
Reference:
J.A.Jamsen,
A.Sassa,
D.D.Shock,
W.A.Beard,
S.H.Wilson.
Dna Polymerase Mu, 8-Oxorgtp:at Product State Ternary Complex, 50 Mm MN2+ (960 Min) To Be Published.
Page generated: Sun Oct 6 07:32:12 2024
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