Manganese in PDB 6uqi: Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*)

All present enzymatic activity of Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*):
2.7.7.7;

The structure of Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*), PDB code: 6uqi was solved by H.Jung, S.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.50
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	98.670, 98.670, 81.808, 90.00, 90.00, 120.00
R / Rfree (%)	17.9 / 23.9

The binding sites of Manganese atom in the Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*) (pdb code 6uqi). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*), PDB code: 6uqi:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn502 b:29.5 occ:1.00 O A:HOH614 2.0 24.2 1.0 OD2 A:ASP13 2.1 29.2 1.0 OE1 A:GLU116 2.1 35.6 1.0 O1A A:XG4501 2.2 28.7 1.0 OD1 A:ASP115 2.3 34.2 1.0 O3' P:DT8 2.4 35.3 1.0 CG A:ASP115 3.0 29.8 1.0 OD2 A:ASP115 3.1 31.1 1.0 C3' P:DT8 3.1 39.5 1.0 PA A:XG4501 3.1 27.3 1.0 CG A:ASP13 3.2 27.9 1.0 CD A:GLU116 3.2 35.7 1.0 O2A A:XG4501 3.5 31.8 1.0 MN A:MN503 3.6 27.9 1.0 OD1 A:ASP13 3.6 25.5 1.0 O5' A:XG4501 3.6 33.3 1.0 OE2 A:GLU116 3.9 37.4 1.0 OG A:SER113 3.9 33.5 1.0 C4' P:DT8 4.1 41.6 1.0 C5' A:XG4501 4.1 28.1 1.0 CG A:GLU116 4.2 33.0 1.0 O A:HOH615 4.2 30.1 1.0 CB A:GLU116 4.3 29.5 1.0 C2' P:DT8 4.4 38.8 1.0 CB A:ASP13 4.4 30.5 1.0 CB A:ASP115 4.5 26.3 1.0 NZ A:LYS224 4.5 41.5 1.0 O2G A:XG4501 4.6 25.4 1.0 C A:ASP115 4.7 30.7 1.0 N3A A:XG4501 4.7 27.2 1.0 O1B A:XG4501 4.7 25.3 1.0 C5' P:DT8 4.7 41.8 1.0 CB A:SER113 4.8 34.6 1.0 N A:GLU116 4.8 33.9 1.0 O A:ASP115 4.8 32.1 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8- Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn503 b:27.9 occ:1.00 O1B A:XG4501 2.0 25.3 1.0 OD2 A:ASP115 2.1 31.1 1.0 O A:MET14 2.1 26.7 1.0 OD1 A:ASP13 2.1 25.5 1.0 O2G A:XG4501 2.1 25.4 1.0 O1A A:XG4501 2.2 28.7 1.0 CG A:ASP13 3.0 27.9 1.0 PB A:XG4501 3.1 27.2 1.0 CG A:ASP115 3.3 29.8 1.0 PA A:XG4501 3.3 27.3 1.0 C A:MET14 3.3 32.1 1.0 OD2 A:ASP13 3.3 29.2 1.0 PG A:XG4501 3.3 29.7 1.0 MN A:MN502 3.6 29.5 1.0 N3A A:XG4501 3.6 27.2 1.0 O3B A:XG4501 3.6 25.1 1.0 N A:MET14 3.7 28.0 1.0 OD1 A:ASP115 3.9 34.2 1.0 NZ A:LYS231 3.9 30.0 1.0 O3G A:XG4501 3.9 27.8 1.0 O A:HOH615 3.9 30.1 1.0 CA A:MET14 4.0 30.9 1.0 C5' A:XG4501 4.1 28.1 1.0 O5' A:XG4501 4.1 33.3 1.0 C A:ASP13 4.2 29.5 1.0 O A:HOH614 4.2 24.2 1.0 CB A:ASP13 4.3 30.5 1.0 N A:ASP15 4.4 29.9 1.0 CB A:ASP115 4.4 26.3 1.0 O2B A:XG4501 4.4 31.2 1.0 N A:CYS16 4.5 30.9 1.0 CE A:LYS231 4.5 29.6 1.0 O1G A:XG4501 4.6 30.1 1.0 O2A A:XG4501 4.6 31.8 1.0 CA A:ASP15 4.6 31.0 1.0 CB A:MET14 4.6 28.2 1.0 O A:ASP13 4.7 28.4 1.0 CA A:ASP13 4.7 29.5 1.0 C A:ASP15 4.8 31.3 1.0 N A:PHE17 4.8 28.6 1.0 O A:ASP115 4.9 32.1 1.0 CB A:PHE17 4.9 24.8 1.0

H.Jung, S.Lee. Crystal Structure of Q38A Human Dna Polymerase Eta Bound with 8-Oxoadenine (Oxoa) and Non-Hydrolyzable Dgtp Analog (Dgtp*) To Be Published.