Manganese in PDB 6s91: Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2

The structure of Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2 also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Manganese atom in the Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2 (pdb code 6s91). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2, PDB code: 6s91:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in the Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2 within 5.0Å range: probe atom residue distance (Å) B Occ K:Mn1104 b:76.0 occ:1.00 O2B K:ANP1103 1.8 65.8 1.0 O K:SER651 2.2 55.8 1.0 O1G K:ANP1103 2.5 65.8 1.0 C K:SER651 3.2 55.8 1.0 PB K:ANP1103 3.2 65.8 1.0 N K:SER651 3.6 55.8 1.0 PG K:ANP1103 3.7 65.8 1.0 OD2 K:ASP650 3.8 52.5 1.0 O3A K:ANP1103 3.8 65.8 1.0 CB K:ASP650 3.8 52.5 1.0 CA K:SER651 3.9 55.8 1.0 C K:ASP650 4.0 52.5 1.0 CG K:ASP650 4.1 52.5 1.0 O1A K:ANP1103 4.1 65.8 1.0 O2G K:ANP1103 4.1 65.8 1.0 N3B K:ANP1103 4.1 65.8 1.0 O1B K:ANP1103 4.2 65.8 1.0 N K:ASP652 4.3 56.6 1.0 CB K:SER651 4.3 55.8 1.0 NZ K:LYS885 4.3 47.9 1.0 OD2 K:ASP803 4.4 53.8 1.0 CA K:ASP650 4.5 52.5 1.0 O2' K:ANP1103 4.5 65.8 1.0 PA K:ANP1103 4.5 65.8 1.0 C2' K:ANP1103 4.5 65.8 1.0 N K:TYR653 4.6 55.2 1.0 CA K:ASP652 4.6 56.6 1.0 O K:ASP650 4.7 52.5 1.0 C3' K:ANP1103 4.7 65.8 1.0 N K:LEU654 4.9 53.0 1.0 O3G K:ANP1103 4.9 65.8 1.0 C K:ASP652 4.9 56.6 1.0

Manganese binding site 2 out of 3 in the Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2 within 5.0Å range: probe atom residue distance (Å) B Occ K:Mn1105 b:87.3 occ:1.00 O1A K:ANP1103 1.9 65.8 1.0 OE1 K:GLU882 2.2 45.5 1.0 PA K:ANP1103 3.1 65.8 1.0 CD K:GLU882 3.3 45.5 1.0 O3G K:ANP1103 3.4 65.8 1.0 O2A K:ANP1103 3.5 65.8 1.0 O5' K:ANP1103 3.7 65.8 1.0 O1G K:ANP1103 3.8 65.8 1.0 OE2 K:GLU882 3.9 45.5 1.0 PG K:ANP1103 4.0 65.8 1.0 N3B K:ANP1103 4.1 65.8 1.0 O3A K:ANP1103 4.4 65.8 1.0 OE2 K:GLU886 4.4 50.5 1.0 CG K:GLU882 4.5 45.5 1.0 PB K:ANP1103 4.8 65.8 1.0 OD2 K:ASP650 4.8 52.5 1.0

Manganese binding site 3 out of 3 in the Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Cryo-Em Structure of the Type III-B Cmr-Beta Bound to Cognate Target Rna and Amppnp, State 2 within 5.0Å range: probe atom residue distance (Å) B Occ K:Mn1106 b:54.4 occ:1.00 O1A K:ANP1102 1.7 47.8 1.0 O3G K:ANP1102 1.7 47.8 1.0 O K:THR207 1.7 49.4 1.0 PA K:ANP1102 2.4 47.8 1.0 O3A K:ANP1102 2.4 47.8 1.0 OD2 K:ASP206 2.7 53.6 1.0 C K:THR207 3.0 49.4 1.0 PG K:ANP1102 3.1 47.8 1.0 O2A K:ANP1102 3.3 47.8 1.0 PB K:ANP1102 3.5 47.8 1.0 CG K:ASP206 3.6 53.6 1.0 N3B K:ANP1102 3.7 47.8 1.0 CG2 K:VAL210 3.7 45.1 1.0 O5' K:ANP1102 3.8 47.8 1.0 N K:ILE208 3.9 48.2 1.0 N K:THR207 3.9 49.4 1.0 O2G K:ANP1102 3.9 47.8 1.0 CA K:THR207 3.9 49.4 1.0 CA K:ILE208 4.0 48.2 1.0 OD1 K:ASP206 4.0 53.6 1.0 O2B K:ANP1102 4.2 47.8 1.0 O1G K:ANP1102 4.2 47.8 1.0 N K:GLY209 4.3 47.6 1.0 C5' K:ANP1102 4.3 47.8 1.0 CB K:THR207 4.5 49.4 1.0 C K:ILE208 4.5 48.2 1.0 C K:ASP206 4.5 53.6 1.0 CE K:LYS429 4.7 50.7 1.0 N K:VAL210 4.7 45.1 1.0 O1B K:ANP1102 4.8 47.8 1.0 NZ K:LYS429 4.8 50.7 1.0 CB K:ASP206 4.9 53.6 1.0 CB K:VAL210 4.9 45.1 1.0 O K:GLY310 4.9 45.2 1.0

N.Sofos, M.Feng, S.Stella, T.Pape, A.Fuglsang, J.Lin, Q.Huang, Y.Li, Q.She, G.Montoya. Structures of the Cmr-Beta Complex Reveal the Regulation of the Immunity Mechanism of Type III-B Crispr-Cas. Mol.Cell V. 79 741 2020.
ISSN: ISSN 1097-2765
PubMed: 32730741
DOI: 10.1016/J.MOLCEL.2020.07.008