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Manganese in PDB 6rk6: Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity.

Enzymatic activity of Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity.

All present enzymatic activity of Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity.:
3.1.13.3;

Protein crystallography data

The structure of Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity., PDB code: 6rk6 was solved by Y.Piotrowski, K.Berg, D.P.Klebl, I.Leiros, A.N.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 44.58 / 3.15
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 108.322, 108.322, 101.334, 90.00, 90.00, 120.00
R / Rfree (%) 24.5 / 26.6

Manganese Binding Sites:

The binding sites of Manganese atom in the Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity. (pdb code 6rk6). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity., PDB code: 6rk6:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in 6rk6

Go back to Manganese Binding Sites List in 6rk6
Manganese binding site 1 out of 3 in the Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn201

b:99.6
occ:1.00
OD2 A:ASP163 2.4 91.8 1.0
OE2 A:GLU14 2.7 78.6 1.0
OD2 A:ASP12 2.9 71.4 1.0
OD1 A:ASP12 3.3 66.6 1.0
CG A:ASP12 3.5 69.5 1.0
CG A:ASP163 3.5 88.5 1.0
CD A:GLU14 3.6 72.2 1.0
CG A:GLU14 3.8 72.3 1.0
CB A:ASP163 4.1 81.2 1.0
CA A:ALA160 4.2 83.1 1.0
O A:GLN159 4.4 88.7 1.0
OD1 A:ASP163 4.5 86.9 1.0
CB A:ALA160 4.6 84.7 1.0
N A:ALA160 4.7 89.4 1.0
OE1 A:GLU14 4.7 72.2 1.0
C A:GLN159 4.8 90.9 1.0
CB A:ASP12 4.9 70.4 1.0
OE1 A:GLU27 5.0 84.1 1.0

Manganese binding site 2 out of 3 in 6rk6

Go back to Manganese Binding Sites List in 6rk6
Manganese binding site 2 out of 3 in the Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity. within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn201

b:0.2
occ:1.00
OD2 B:ASP163 2.4 99.7 1.0
OD2 B:ASP12 3.4 77.8 1.0
OE2 B:GLU14 3.4 84.1 1.0
CG B:ASP163 3.6 96.3 1.0
CA B:ALA160 3.7 85.9 1.0
O B:GLN159 3.8 89.6 1.0
OD1 B:ASP12 4.0 77.6 1.0
CG B:ASP12 4.0 76.6 1.0
N B:ALA160 4.1 90.2 1.0
C B:GLN159 4.1 92.3 1.0
CD B:GLU14 4.1 76.1 1.0
CG B:GLU14 4.2 72.0 1.0
CB B:ALA160 4.2 86.2 1.0
CB B:ASP163 4.3 89.4 1.0
OD1 B:ASP163 4.5 93.5 1.0
OE1 B:GLU27 4.6 81.9 1.0
O B:HIS158 4.7 0.0 1.0
C B:ALA160 4.9 82.4 1.0

Manganese binding site 3 out of 3 in 6rk6

Go back to Manganese Binding Sites List in 6rk6
Manganese binding site 3 out of 3 in the Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity. within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mn201

b:95.4
occ:1.00
OE2 C:GLU14 2.4 73.6 1.0
OD2 C:ASP163 2.6 85.5 1.0
OD2 C:ASP12 3.0 73.4 1.0
OD1 C:ASP12 3.2 69.2 1.0
CD C:GLU14 3.4 68.7 1.0
CG C:ASP12 3.5 69.9 1.0
CG C:ASP163 3.6 86.5 1.0
CG C:GLU14 3.8 67.0 1.0
CB C:ASP163 4.2 82.2 1.0
OD1 C:ASP163 4.5 86.4 1.0
OE1 C:GLU14 4.5 71.0 1.0
CA C:ALA160 4.6 81.9 1.0
O C:GLN159 4.8 93.9 1.0
CB C:ASP12 4.9 65.9 1.0
CB C:ALA160 4.9 79.9 1.0

Reference:

Y.Piotrowski, K.Berg, D.P.Klebl, I.Leiros, A.N.Larsen. Characterization of An Intertidal Zone Metagenome Oligoribonuclease and the Role of the Intermolecular Disulfide Bond For Homodimer Formation and Nuclease Activity. Febs Open Bio V. 9 1674 2019.
ISSN: ESSN 2211-5463
PubMed: 31420950
DOI: 10.1002/2211-5463.12720
Page generated: Tue Dec 15 05:00:26 2020

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