Manganese in PDB 6p1o: Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp

Enzymatic activity of Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp

All present enzymatic activity of Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp:
2.7.7.7;

Protein crystallography data

The structure of Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp, PDB code: 6p1o was solved by A.M.Kaminski, L.C.Pedersen, K.Bebenek, K.K.Chiruvella, D.A.Ramsden, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	25.15 / 1.65
*Space group*	P 2₁ 2₁ 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	59.947, 68.713, 110.724, 90.00, 90.00, 90.00
*R / R_free (%)*	16.7 / 18.7

Other elements in 6p1o:

The structure of Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Chlorine	(Cl)	3 atoms
Sodium	(Na)	1 atom

Manganese Binding Sites:

The binding sites of Manganese atom in the Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp (pdb code 6p1o). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp, PDB code: 6p1o:

Manganese binding site 1 out of 1 in 6p1o

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Manganese Binding Sites List in 6p1o

Manganese binding site 1 out of 1 in the Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp

Mono view

Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Post-Catalytic Nicked Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG and Newly Incorporated Damp within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mn501 b:14.9 occ:0.73	OD1	A:ASP332	1.8	18.6	1.0
	OD2	A:ASP330	1.8	22.4	1.0
	OD2	A:ASP418	2.0	19.9	1.0
	OP1	P:DA5	2.1	21.1	1.0
	CG	A:ASP330	2.8	24.4	1.0
	CG	A:ASP332	2.8	17.4	1.0
	O3'	P:DA4	3.0	24.5	1.0
	CG	A:ASP418	3.0	19.2	1.0
	OD1	A:ASP330	3.0	18.2	1.0
	P	P:DA5	3.1	21.6	1.0
	OD2	A:ASP332	3.2	17.3	1.0
	MG	A:MG502	3.4	19.6	1.0
	CB	A:ASP418	3.6	16.3	1.0
	OD1	A:ASP418	4.0	19.5	1.0
	C3'	P:DA4	4.2	22.2	1.0
	CB	A:ASP332	4.2	13.7	1.0
	CB	A:ASP330	4.2	19.7	1.0
	O5'	P:DA5	4.2	16.1	1.0
	OP2	P:DA5	4.2	21.6	1.0
	C5'	P:DA5	4.2	16.2	1.0
	C4'	P:DA4	4.2	20.1	1.0
	C5'	P:DA4	4.3	20.1	1.0
	CE1	A:HIS329	4.5	26.1	0.5
	O	A:VAL331	4.6	16.5	1.0
	NH2	A:ARG416	4.6	21.0	1.0
	CA	A:ASP332	4.7	14.1	1.0
	O32	A:PPV507	4.7	35.2	0.6
	CZ3	A:TRP434	4.7	22.1	1.0
	N	A:ASP418	4.7	17.0	1.0
	C	A:VAL331	4.8	15.6	1.0
	O2B	P:ATP701	4.8	35.2	0.4
	CA	A:ASP418	4.8	15.8	1.0
	N	A:ASP332	4.9	13.8	1.0
	O2G	P:ATP701	5.0	19.2	0.4

Reference:

A.M.Kaminski, K.K.Chiruvella, D.A.Ramsden, T.A.Kunkel, K.Bebenek, L.C.Pedersen. Unexpected Behavior of Dna Polymerase Mu Opposite Template 8-Oxo-7,8-Dihydro-2'-Guanosine. Nucleic Acids Res. V. 47 9410 2019.
ISSN: ESSN 1362-4962
PubMed: 31435651
DOI: 10.1093/NAR/GKZ680

Page generated: Tue Dec 15 04:58:43 2020

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