Manganese in PDB 6iph: Binary Complex of Human Dna Polymerase Mu with Mndatp

All present enzymatic activity of Binary Complex of Human Dna Polymerase Mu with Mndatp:
2.7.7.7;

The structure of Binary Complex of Human Dna Polymerase Mu with Mndatp, PDB code: 6iph was solved by Y.K.Chang, W.J.Wu, M.D.Tsai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.65
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	124.582, 124.582, 50.548, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 21.6

The binding sites of Manganese atom in the Binary Complex of Human Dna Polymerase Mu with Mndatp (pdb code 6iph). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Binary Complex of Human Dna Polymerase Mu with Mndatp, PDB code: 6iph:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Binary Complex of Human Dna Polymerase Mu with Mndatp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Binary Complex of Human Dna Polymerase Mu with Mndatp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:24.2 occ:1.00 OD1 A:ASP330 2.0 21.5 1.0 O1A A:DTP503 2.1 32.1 1.0 O1B A:DTP503 2.2 27.8 1.0 OD2 A:ASP332 2.2 21.5 1.0 O3G A:DTP503 2.2 39.3 1.0 O A:HOH765 2.3 22.1 1.0 CG A:ASP330 3.1 21.4 1.0 CG A:ASP332 3.2 19.7 1.0 PA A:DTP503 3.3 32.3 1.0 PB A:DTP503 3.3 30.7 1.0 PG A:DTP503 3.4 40.1 1.0 O3A A:DTP503 3.5 32.5 1.0 OD2 A:ASP330 3.5 21.1 1.0 OD1 A:ASP332 3.5 20.3 1.0 MN A:MN502 3.7 29.0 1.0 O3B A:DTP503 3.8 33.2 1.0 CE1 A:HIS329 3.9 31.6 1.0 O1G A:DTP503 4.0 42.9 1.0 O A:ASP330 4.0 18.3 1.0 ND1 A:HIS329 4.1 32.0 1.0 O A:HOH715 4.2 18.7 1.0 O5' A:DTP503 4.3 36.8 1.0 C A:ASP330 4.3 19.0 1.0 O2A A:DTP503 4.3 32.4 1.0 N A:GLY320 4.4 18.7 1.0 CA A:GLY319 4.5 18.7 1.0 CB A:ASP330 4.5 20.3 1.0 CB A:ASP332 4.5 18.5 1.0 C5' A:DTP503 4.6 40.1 1.0 O2B A:DTP503 4.7 31.2 1.0 N A:ASP330 4.7 19.9 1.0 O2G A:DTP503 4.7 43.5 1.0 N A:ASP332 4.7 17.4 1.0 CA A:ASP330 4.7 19.6 1.0 O A:HOH803 4.8 35.4 1.0 O A:HOH601 4.8 39.9 1.0 N A:VAL331 4.8 17.9 1.0 C8 A:DTP503 4.9 43.8 1.0

Manganese binding site 2 out of 2 in the Binary Complex of Human Dna Polymerase Mu with Mndatp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Binary Complex of Human Dna Polymerase Mu with Mndatp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn502 b:29.0 occ:1.00 OD2 A:ASP330 2.1 21.1 1.0 OD1 A:ASP332 2.1 20.3 1.0 OD2 A:ASP418 2.2 23.9 1.0 N7 A:DTP503 2.3 42.6 1.0 O A:HOH803 2.3 35.4 1.0 O1A A:DTP503 2.7 32.1 1.0 C8 A:DTP503 3.1 43.8 1.0 CG A:ASP330 3.1 21.4 1.0 CG A:ASP332 3.2 19.7 1.0 CG A:ASP418 3.3 21.6 1.0 OD1 A:ASP330 3.4 21.5 1.0 C5 A:DTP503 3.5 44.0 1.0 OD2 A:ASP332 3.6 21.5 1.0 MN A:MN501 3.7 24.2 1.0 PA A:DTP503 3.9 32.3 1.0 CB A:ASP418 4.0 20.3 1.0 N6 A:DTP503 4.0 45.6 1.0 O2A A:DTP503 4.2 32.4 1.0 C6 A:DTP503 4.2 44.9 1.0 OD1 A:ASP418 4.3 23.6 1.0 O A:HOH672 4.3 39.2 1.0 N9 A:DTP503 4.4 42.8 1.0 CE1 A:HIS329 4.4 31.6 1.0 CB A:ASP330 4.4 20.3 1.0 CB A:ASP332 4.5 18.5 1.0 O5' A:DTP503 4.5 36.8 1.0 CZ3 A:TRP434 4.5 27.6 1.0 C4 A:DTP503 4.6 44.0 1.0 NE2 A:HIS329 4.8 33.1 1.0 O A:HOH670 4.8 43.1 1.0

Y.K.Chang, Y.P.Huang, X.X.Liu, T.P.Ko, Y.Bessho, Y.Kawano, M.Maestre-Reyna, W.J.Wu, M.D.Tsai. Human Dna Polymerase Mu Can Use A Noncanonical Mechanism For Multiple MN2+-Mediated Functions. J.Am.Chem.Soc. V. 141 8489 2019.
ISSN: ESSN 1520-5126
PubMed: 31067051
DOI: 10.1021/JACS.9B01741