Manganese in PDB 6e7i: Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp

All present enzymatic activity of Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp:
2.4.1.41;

The structure of Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp, PDB code: 6e7i was solved by C.R.Bertozzi, B.Schumann, A.J.Agbay, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.66 / 1.80
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	69.310, 69.310, 169.780, 90.00, 90.00, 120.00
R / Rfree (%)	16.7 / 21.6

The binding sites of Manganese atom in the Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp (pdb code 6e7i). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp, PDB code: 6e7i:

Manganese binding site 1 out of 1 in the Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Human PPGALNACT2 I253A/L310A Mutant with EA2 and Udp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn602 b:10.1 occ:1.00 O2A A:UDP601 2.1 11.0 1.0 O2B A:UDP601 2.2 12.0 1.0 NE2 A:HIS226 2.2 7.5 1.0 NE2 A:HIS359 2.2 8.3 1.0 OD2 A:ASP224 2.3 10.8 1.0 O A:HOH855 2.5 13.8 1.0 CE1 A:HIS226 3.1 7.8 1.0 CE1 A:HIS359 3.2 9.5 1.0 CD2 A:HIS359 3.3 8.8 1.0 PB A:UDP601 3.3 13.4 1.0 CD2 A:HIS226 3.3 7.2 1.0 PA A:UDP601 3.3 11.9 1.0 CG A:ASP224 3.4 9.9 1.0 O A:HOH705 3.5 12.4 1.0 O3A A:UDP601 3.6 11.9 1.0 O3B A:UDP601 3.8 15.6 1.0 CB A:ASP224 3.9 9.0 1.0 ND1 A:HIS226 4.3 7.3 1.0 O5' A:UDP601 4.3 9.3 1.0 C5' A:UDP601 4.3 9.9 1.0 ND1 A:HIS359 4.3 9.6 1.0 O A:VAL360 4.3 9.8 1.0 CG A:HIS359 4.4 8.8 1.0 CG A:HIS226 4.4 7.6 1.0 OD1 A:ASP224 4.4 9.7 1.0 O1B A:UDP601 4.5 12.6 1.0 O1A A:UDP601 4.6 11.0 1.0 C3' A:UDP601 4.7 10.4 1.0 NH2 A:ARG362 4.8 15.0 1.0 C4' A:UDP601 4.8 10.0 1.0 O A:HOH889 4.9 7.8 1.0

C.R.Bertozzi, B.Schumann, A.J.Agbay. Structure of Bump-Hole Engineered Human PPGALNACT2 with Bound EA2 and Udp To Be Published.