Atomistry » Manganese » PDB 6dkt-6e4c » 6dpd
Atomistry »
  Manganese »
    PDB 6dkt-6e4c »
      6dpd »

Manganese in PDB 6dpd: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dpd was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.84 / 1.46
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.324, 37.988, 61.323, 90.00, 96.32, 90.00
R / Rfree (%) 15.1 / 17.3

Other elements in 6dpd:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Potassium (K) 1 atom
Iodine (I) 5 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C (pdb code 6dpd). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 6 binding sites of Manganese where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dpd:
Jump to Manganese binding site number: 1; 2; 3; 4; 5; 6;

Manganese binding site 1 out of 6 in 6dpd

Go back to Manganese Binding Sites List in 6dpd
Manganese binding site 1 out of 6 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn201

b:20.4
occ:1.00
O A:HOH359 2.1 21.2 1.0
OD2 A:ASP71 2.1 17.9 1.0
OD1 A:ASP192 2.1 22.4 1.0
OP3 b:C5 2.1 24.3 1.0
O A:HOH321 2.2 19.3 1.0
OP1 b:C5 2.6 18.8 1.0
P b:C5 2.9 21.8 1.0
HA A:ASP192 3.0 28.3 1.0
CG A:ASP71 3.1 16.7 1.0
CG A:ASP192 3.2 24.6 1.0
HB2 A:ASP192 3.4 27.7 1.0
OD1 A:ASP71 3.5 16.8 1.0
H A:TYR193 3.5 29.6 1.0
CB A:ASP192 3.6 23.1 1.0
MN A:MN203 3.7 32.2 0.1
CA A:ASP192 3.7 23.6 1.0
MN A:MN202 3.8 19.4 1.0
NZ A:LYS196 3.9 25.8 0.8
O5' b:C5 4.0 20.0 1.0
OP2 b:C5 4.0 21.4 1.0
OD2 A:ASP132 4.0 27.9 1.0
OP2 b:G6 4.0 21.4 1.0
O B:HOH207 4.0 26.1 1.0
HG22 A:ILE189 4.1 24.9 1.0
O A:VAL72 4.1 19.8 1.0
OE2 A:GLU188 4.2 32.5 1.0
C5' b:C5 4.2 23.5 1.0
N A:TYR193 4.2 24.7 1.0
OD2 A:ASP192 4.3 25.9 1.0
CB A:ASP71 4.4 15.9 1.0
HD1 A:TYR193 4.5 27.3 1.0
HB3 A:ASP192 4.6 27.7 1.0
C A:ASP192 4.6 25.2 1.0
HG21 A:ILE189 4.7 24.9 1.0
CG2 A:ILE189 4.7 20.8 1.0
N A:ASP192 4.8 21.9 1.0
K A:K206 4.8 34.0 0.6
HG23 A:ILE189 4.9 24.9 1.0
HB2 A:TYR193 4.9 30.6 1.0

Manganese binding site 2 out of 6 in 6dpd

Go back to Manganese Binding Sites List in 6dpd
Manganese binding site 2 out of 6 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn202

b:19.4
occ:1.00
OP1 b:C5 2.0 18.8 1.0
OD1 A:ASP71 2.0 16.8 1.0
OD1 A:ASP132 2.1 20.5 1.0
OE1 A:GLU109 2.1 18.2 1.0
OD2 A:ASP132 2.5 27.9 1.0
CG A:ASP132 2.6 21.3 1.0
CD A:GLU109 3.0 19.2 1.0
CG A:ASP71 3.1 16.7 1.0
O A:HOH352 3.2 18.6 1.0
OE2 A:GLU109 3.3 20.1 1.0
P b:C5 3.4 21.8 1.0
OD2 A:ASP71 3.5 17.9 1.0
HG12 A:VAL72 3.6 23.2 1.0
C3' B:U4 3.6 23.7 1.0
O3' B:U4 3.6 24.0 1.0
H A:VAL72 3.8 20.8 1.0
MN A:MN201 3.8 20.4 1.0
O A:HOH359 4.0 21.2 1.0
C5' b:C5 4.1 23.5 1.0
CB A:ASP132 4.1 21.1 1.0
H A:ASP132 4.1 21.5 1.0
O5' b:C5 4.2 20.0 1.0
OP2 b:C5 4.2 21.4 1.0
HB2 A:SER133 4.3 24.9 1.0
CB A:ASP71 4.3 15.9 1.0
OP3 b:C5 4.3 24.3 1.0
HG21 A:ILE189 4.4 24.9 1.0
CG A:GLU109 4.4 16.6 1.0
CG1 A:VAL72 4.5 19.4 1.0
N A:VAL72 4.5 17.3 1.0
HB3 A:ASP132 4.6 25.3 1.0
HB2 A:ASP132 4.6 25.3 1.0
C4' B:U4 4.6 22.8 1.0
CA A:ASP71 4.6 15.7 1.0
HG3 A:GLU109 4.6 19.9 1.0
C2' B:U4 4.7 21.2 1.0
HG2 A:GLU109 4.7 19.9 1.0
C5' B:U4 4.8 23.0 1.0
CA A:ASP132 4.8 19.9 1.0
N A:ASP132 4.8 17.9 1.0
HG13 A:VAL72 4.8 23.2 1.0
HG22 A:ILE189 4.8 24.9 1.0
C A:ASP132 4.9 19.0 1.0
O2' B:U4 4.9 24.7 1.0
HG11 A:VAL72 4.9 23.2 1.0
O A:VAL72 4.9 19.8 1.0
O A:HOH321 4.9 19.3 1.0

Manganese binding site 3 out of 6 in 6dpd

Go back to Manganese Binding Sites List in 6dpd
Manganese binding site 3 out of 6 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn203

b:32.2
occ:0.15
OP3 b:C5 2.1 24.3 1.0
CG A:LYS196 2.3 32.2 0.8
CD A:LYS196 2.4 30.4 0.8
NZ A:LYS196 2.4 25.8 0.8
OP2 b:G6 2.5 21.4 1.0
O B:HOH207 2.7 26.1 1.0
CE A:LYS196 2.9 27.2 0.8
P b:C5 3.2 21.8 1.0
O5' b:C5 3.4 20.0 1.0
CB A:LYS196 3.6 36.3 0.8
O5' b:G6 3.6 23.1 1.0
P b:G6 3.7 22.2 1.0
OD1 A:ASP192 3.7 22.4 1.0
MN A:MN201 3.7 20.4 1.0
O A:HOH321 3.8 19.3 1.0
OP2 b:C5 3.8 21.4 1.0
O A:HOH378 3.9 28.5 1.0
C5' b:C5 4.1 23.5 1.0
C3' b:C5 4.2 20.2 1.0
N A:LYS196 4.3 33.7 0.8
OP1 b:C5 4.4 18.8 1.0
O3' b:C5 4.4 20.7 1.0
CA A:LYS196 4.5 37.3 0.8
C4' b:C5 4.7 21.6 1.0
CG A:ASP192 4.7 24.6 1.0
O A:HOH359 4.8 21.2 1.0
OP1 b:G6 4.9 21.6 1.0
C6 b:C5 4.9 21.1 1.0
C5' b:G6 5.0 24.3 1.0
C A:ARG195 5.0 35.2 1.0

Manganese binding site 4 out of 6 in 6dpd

Go back to Manganese Binding Sites List in 6dpd
Manganese binding site 4 out of 6 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn204

b:27.4
occ:0.60
OE1 A:GLU166 2.1 27.7 1.0
O A:HOH415 2.2 39.3 1.0
O A:HOH346 2.4 37.0 1.0
OD1 A:ASP163 2.5 29.4 1.0
CD A:GLU166 3.1 39.1 1.0
HA A:ASP163 3.4 23.2 1.0
OE2 A:GLU166 3.5 25.6 1.0
CG A:ASP163 3.5 29.9 1.0
NH2 A:ARG151 4.0 35.5 1.0
O A:HOH384 4.2 38.6 1.0
NZ A:LYS144 4.2 67.8 1.0
CA A:ASP163 4.2 19.4 1.0
HB2 A:GLU166 4.2 24.3 1.0
CB A:ASP163 4.2 24.3 1.0
HZ3 A:TRP159 4.2 31.2 1.0
HB3 A:ASP163 4.3 29.2 1.0
OD2 A:ASP163 4.4 28.6 1.0
CG A:GLU166 4.4 24.1 1.0
HG12 A:VAL162 4.5 27.5 1.0
CB A:GLU166 4.7 20.2 1.0
HB3 A:GLU166 4.7 24.3 1.0
HG3 A:GLU166 4.8 28.9 1.0
N A:ASP163 4.9 18.4 1.0
HE3 A:TRP159 5.0 28.1 1.0

Manganese binding site 5 out of 6 in 6dpd

Go back to Manganese Binding Sites List in 6dpd
Manganese binding site 5 out of 6 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn205

b:35.5
occ:0.50
O A:LYS196 2.2 46.8 0.8
O A:HOH392 3.0 56.9 1.0
O A:HOH314 3.3 56.7 1.0
C A:LYS196 3.4 35.6 0.8
OXT A:LYS196 3.9 47.2 0.8
MN B:MN101 4.1 50.0 0.6
CA A:LYS196 4.6 37.3 0.8

Manganese binding site 6 out of 6 in 6dpd

Go back to Manganese Binding Sites List in 6dpd
Manganese binding site 6 out of 6 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 6 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 16 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn101

b:50.0
occ:0.60
O B:HOH212 2.5 52.6 1.0
O A:HOH314 2.6 56.7 1.0
O6 b:G6 2.7 35.4 1.0
O B:HOH210 2.8 55.9 1.0
N7 b:G6 3.6 32.0 1.0
C6 b:G6 3.6 29.6 1.0
C5 b:G6 3.9 28.1 1.0
MN A:MN205 4.1 35.5 0.5
O A:LYS196 4.1 46.8 0.8
O B:HOH213 4.6 33.8 1.0
N4 C:DC1 4.6 30.1 1.0
CB A:LYS196 4.8 36.3 0.8
C8 b:G6 4.8 28.4 1.0
N4 b:C5 4.8 29.9 1.0
N1 b:G6 4.9 30.8 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sun Oct 6 04:06:46 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy