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Manganese in PDB 6dpb: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dpb was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 33.89 / 1.32
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.546, 37.318, 62.154, 90.00, 96.85, 90.00
R / Rfree (%) 14.8 / 17.2

Other elements in 6dpb:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 4 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C (pdb code 6dpb). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dpb:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in 6dpb

Go back to Manganese Binding Sites List in 6dpb
Manganese binding site 1 out of 4 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn201

b:19.3
occ:1.00
OD1 A:ASP192 2.1 21.5 1.0
OD2 A:ASP71 2.1 18.3 1.0
O b:HOH201 2.1 20.7 0.4
OP3 b:C5 2.1 23.2 0.6
O A:HOH351 2.2 20.9 1.0
O A:HOH327 2.2 20.2 1.0
OP1 b:C5 2.3 16.4 0.4
OP1 b:C5 2.4 36.7 0.6
P b:C5 2.9 16.7 0.6
HA A:ASP192 3.1 25.3 1.0
CG A:ASP71 3.1 17.0 1.0
CG A:ASP192 3.2 20.0 1.0
OD1 A:ASP71 3.5 17.8 1.0
P b:C5 3.5 20.1 0.4
HB2 A:ASP192 3.5 24.2 1.0
H A:TYR193 3.6 27.4 1.0
CB A:ASP192 3.7 20.2 1.0
MN A:MN202 3.8 17.9 1.0
CA A:ASP192 3.8 21.1 1.0
OP2 b:C5 3.8 28.0 0.4
K A:K204 3.9 24.3 0.2
O5' b:C5 3.9 16.2 0.6
NZ A:LYS196 3.9 22.0 0.6
OP2 b:G6 4.0 21.0 1.0
OP2 b:C5 4.0 16.9 0.6
OD2 A:ASP132 4.0 24.5 1.0
HG22 A:ILE189 4.1 22.6 1.0
O A:HOH366 4.1 18.8 0.6
O A:VAL72 4.1 17.6 1.0
C5' b:C5 4.2 19.6 1.0
OD2 A:ASP192 4.2 25.1 1.0
OE2 A:GLU188 4.3 32.0 1.0
O5' b:C5 4.3 17.2 0.4
N A:TYR193 4.3 22.8 1.0
CB A:ASP71 4.4 16.0 1.0
HD1 A:TYR193 4.5 26.2 1.0
O A:HOH310 4.6 19.0 0.4
O3' B:U4 4.6 23.7 0.4
C A:ASP192 4.7 23.0 1.0
HB3 A:ASP192 4.7 24.2 1.0
HG21 A:ILE189 4.7 22.6 1.0
CG2 A:ILE189 4.7 18.8 1.0
HG23 A:ILE189 4.9 22.6 1.0
N A:ASP192 4.9 19.5 1.0
K A:K216 4.9 22.9 0.3
HB2 A:TYR193 5.0 26.9 1.0

Manganese binding site 2 out of 4 in 6dpb

Go back to Manganese Binding Sites List in 6dpb
Manganese binding site 2 out of 4 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn202

b:17.9
occ:1.00
OD1 A:ASP71 2.1 17.8 1.0
OD1 A:ASP132 2.1 19.4 1.0
OE1 A:GLU109 2.1 18.6 1.0
OP1 b:C5 2.3 16.4 0.4
OP1 b:C5 2.4 36.7 0.6
OD2 A:ASP132 2.5 24.5 1.0
CG A:ASP132 2.6 20.6 1.0
O3' B:U4 2.7 23.7 0.4
CD A:GLU109 3.0 16.7 1.0
P b:C5 3.1 20.1 0.4
CG A:ASP71 3.1 17.0 1.0
O A:HOH345 3.2 18.2 1.0
OE2 A:GLU109 3.2 17.6 1.0
OD2 A:ASP71 3.5 18.3 1.0
HG12 A:VAL72 3.6 19.5 1.0
C3' B:U4 3.6 16.7 0.6
O3' B:U4 3.6 17.0 0.6
P b:C5 3.6 16.7 0.6
H A:VAL72 3.8 18.2 1.0
MN A:MN201 3.8 19.3 1.0
C3' B:U4 4.0 38.2 0.4
O A:HOH351 4.0 20.9 1.0
O5' b:C5 4.0 17.2 0.4
C5' b:C5 4.1 19.6 1.0
CB A:ASP132 4.1 18.3 1.0
OP2 b:C5 4.1 16.9 0.6
H A:ASP132 4.1 18.6 1.0
OP2 b:C5 4.2 28.0 0.4
HB2 A:SER133 4.3 21.9 1.0
O5' b:C5 4.3 16.2 0.6
CB A:ASP71 4.3 16.0 1.0
HG21 A:ILE189 4.4 22.6 1.0
CG A:GLU109 4.4 15.3 1.0
O2' B:U4 4.4 24.7 0.4
C4' B:U4 4.4 33.5 0.4
OP3 b:C5 4.5 23.2 0.6
CG1 A:VAL72 4.5 16.3 1.0
HG3 A:GLU109 4.6 18.4 1.0
HB2 A:ASP132 4.6 22.0 1.0
N A:VAL72 4.6 15.2 1.0
HB3 A:ASP132 4.6 22.0 1.0
C4' B:U4 4.6 20.2 0.6
O b:HOH201 4.6 20.7 0.4
CA A:ASP71 4.6 16.6 1.0
HG2 A:GLU109 4.7 18.4 1.0
C2' B:U4 4.7 26.1 0.6
C5' B:U4 4.8 31.7 0.4
C2' B:U4 4.8 19.8 0.4
C5' B:U4 4.8 16.8 0.6
N A:ASP132 4.8 15.5 1.0
CA A:ASP132 4.8 17.4 1.0
HG22 A:ILE189 4.8 22.6 1.0
HG13 A:VAL72 4.9 19.5 1.0
C A:ASP132 4.9 18.2 1.0
HG11 A:VAL72 4.9 19.5 1.0
O2' B:U4 4.9 18.1 0.6
K A:K216 4.9 22.9 0.3
O A:VAL72 4.9 17.6 1.0
O A:HOH327 5.0 20.2 1.0

Manganese binding site 3 out of 4 in 6dpb

Go back to Manganese Binding Sites List in 6dpb
Manganese binding site 3 out of 4 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn203

b:23.2
occ:0.40
OE1 A:GLU166 2.1 32.3 1.0
O A:HOH426 2.3 48.0 1.0
O A:HOH302 2.4 51.2 1.0
OD1 A:ASP163 2.6 27.3 1.0
O A:HOH323 2.9 44.7 1.0
CD A:GLU166 3.2 25.9 1.0
HA A:ASP163 3.4 22.4 1.0
OE2 A:GLU166 3.5 26.4 1.0
NZ A:LYS144 3.5 89.6 1.0
CG A:ASP163 3.6 30.1 1.0
NH2 A:ARG151 3.8 29.5 1.0
O A:HOH375 4.0 35.5 1.0
HB2 A:GLU166 4.2 22.6 1.0
CA A:ASP163 4.3 18.6 1.0
HZ3 A:TRP159 4.3 29.0 1.0
CB A:ASP163 4.4 21.2 1.0
HB3 A:ASP163 4.4 25.4 1.0
CG A:GLU166 4.5 20.7 1.0
OD2 A:ASP163 4.5 29.1 1.0
HG12 A:VAL162 4.5 25.4 1.0
CE A:LYS144 4.7 77.1 1.0
CB A:GLU166 4.8 18.8 1.0
HB3 A:GLU166 4.8 22.6 1.0
HG3 A:GLU166 4.8 24.8 1.0
CD A:LYS144 4.9 61.6 1.0
N A:ASP163 4.9 18.8 1.0

Manganese binding site 4 out of 4 in 6dpb

Go back to Manganese Binding Sites List in 6dpb
Manganese binding site 4 out of 4 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 8 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
b:Mn101

b:31.1
occ:0.20
O b:HOH202 2.4 38.5 1.0
O6 b:G6 2.7 28.8 1.0
O b:HOH205 2.9 49.1 1.0
C6 b:G6 3.6 25.8 1.0
N7 b:G6 3.6 30.4 1.0
C5 b:G6 3.9 24.7 1.0
O A:LYS196 4.2 58.8 0.6
N4 C:DC1 4.5 29.2 1.0
O b:HOH206 4.7 31.3 1.0
N1 b:G6 4.8 24.2 1.0
C8 b:G6 4.9 28.0 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Tue Dec 15 04:51:50 2020

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