Manganese in PDB 5zfs: Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose

The structure of Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose, PDB code: 5zfs was solved by H.Yoshida, A.Yoshihara, P.K.Gullapalli, K.Ohtani, K.Akimitsu, K.Izumori, S.Kamitori, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.02 / 1.96
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	103.980, 103.980, 256.530, 90.00, 90.00, 120.00
R / Rfree (%)	18.6 / 21.1

The binding sites of Manganese atom in the Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose (pdb code 5zfs). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose, PDB code: 5zfs:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn301 b:39.6 occ:1.00 OE2 A:GLU146 2.1 19.0 1.0 O A:HOH433 2.2 21.9 0.5 ND1 A:HIS205 2.4 18.7 1.0 OD2 A:ASP179 2.4 18.3 1.0 OE1 A:GLU240 2.4 20.5 1.0 O A:HOH559 2.7 46.0 1.0 CD A:GLU146 3.2 19.1 1.0 CE1 A:HIS205 3.2 18.5 1.0 CD A:GLU240 3.4 20.8 1.0 CG A:ASP179 3.5 17.8 1.0 OE1 A:GLU146 3.5 19.6 1.0 CG A:HIS205 3.6 17.9 1.0 OE2 A:GLU240 3.6 21.5 1.0 NH2 A:ARG211 3.8 19.9 1.0 CB A:ASP179 3.9 16.8 1.0 CB A:HIS205 4.0 17.4 1.0 O A:HOH474 4.0 22.4 1.0 O A:HOH433 4.1 11.7 0.5 NE2 A:HIS205 4.4 18.6 1.0 NE2 A:HIS182 4.4 15.6 1.0 CD2 A:HIS182 4.4 15.7 1.0 CG A:GLU146 4.5 18.4 1.0 OD1 A:ASP179 4.5 18.2 1.0 NE2 A:HIS177 4.5 17.5 1.0 CD2 A:HIS205 4.6 18.6 1.0 CE1 A:HIS177 4.6 17.6 1.0 CG A:GLU240 4.7 19.9 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Arthrobacter Globiformis M30 Sugar Epimerase Which Can Produce D-Allulose From D-Fructose within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn301 b:45.9 occ:1.00 OE2 B:GLU146 2.1 18.5 1.0 OD2 B:ASP179 2.3 17.7 1.0 OE1 B:GLU240 2.5 21.6 1.0 ND1 B:HIS205 2.5 18.9 1.0 O B:HOH417 2.8 21.3 0.5 O B:HOH473 3.0 30.9 1.0 CD B:GLU146 3.2 19.1 1.0 CE1 B:HIS205 3.3 19.1 1.0 CG B:ASP179 3.4 17.6 1.0 CD B:GLU240 3.5 21.4 1.0 OE1 B:GLU146 3.5 19.6 1.0 CG B:HIS205 3.6 18.6 1.0 NH2 B:ARG211 3.6 19.4 1.0 OE2 B:GLU240 3.8 22.5 1.0 CB B:ASP179 3.9 17.1 1.0 CB B:HIS205 4.0 17.9 1.0 O B:HOH519 4.1 26.2 1.0 O B:HOH417 4.1 7.8 0.5 NE2 B:HIS182 4.4 16.2 1.0 OD1 B:ASP179 4.4 18.3 1.0 CG B:GLU146 4.4 18.5 1.0 CD2 B:HIS182 4.4 16.3 1.0 NE2 B:HIS205 4.5 19.8 1.0 CE1 B:HIS177 4.5 18.4 1.0 NE2 B:HIS177 4.5 18.8 1.0 CD2 B:HIS205 4.6 19.2 1.0 CG B:GLU240 4.8 20.5 1.0 CZ B:ARG211 5.0 18.4 1.0

H.Yoshida, A.Yoshihara, P.K.Gullapalli, K.Ohtani, K.Akimitsu, K.Izumori, S.Kamitori. X-Ray Structure of Arthrobacter Globiformis M30 Ketose 3-Epimerase For the Production of D-Allulose From D-Fructose. Acta Crystallogr F Struct V. 74 669 2018BIOL Commun.
ISSN: ESSN 2053-230X
PubMed: 30279320
DOI: 10.1107/S2053230X18011706