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Manganese in PDB 5mof: Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate

Enzymatic activity of Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate

All present enzymatic activity of Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate:
1.13.12.19; 1.14.11.34;

Protein crystallography data

The structure of Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate, PDB code: 5mof was solved by M.A.Mcdonough, Z.Zhang, C.J.Schofield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 69.28 / 1.45
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 79.484, 97.850, 98.094, 90.00, 90.00, 90.00
R / Rfree (%) 17.2 / 18.9

Other elements in 5mof:

The structure of Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Manganese Binding Sites:

The binding sites of Manganese atom in the Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate (pdb code 5mof). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate, PDB code: 5mof:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 5mof

Go back to Manganese Binding Sites List in 5mof
Manganese binding site 1 out of 2 in the Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn401

b:14.0
occ:0.85
 O A:HOH841 2.0 18.9 0.3
O2 A:AKG404 2.0 19.1 0.7
NE2 A:HIS268 2.0 13.2 1.0
O5 A:AKG404 2.1 18.2 0.7
OD1 A:ASP191 2.2 13.2 1.0
O A:HOH715 2.2 19.4 0.3
NE2 A:HIS189 2.2 11.3 1.0
O A:HOH518 2.2 17.9 1.0
C1 A:AKG404 2.6 20.3 0.7
C2 A:AKG404 2.6 19.9 0.7
CD2 A:HIS268 3.0 12.2 1.0
CG A:ASP191 3.0 12.2 1.0
CE1 A:HIS268 3.1 12.8 1.0
CE1 A:HIS189 3.1 11.3 1.0
OD2 A:ASP191 3.1 13.0 1.0
CD2 A:HIS189 3.3 11.3 1.0
O1 A:AKG404 3.8 21.3 0.7
O A:HOH863 3.9 21.7 0.3
C3 A:AKG404 4.0 20.2 0.7
CG A:HIS268 4.1 11.2 1.0
ND1 A:HIS268 4.1 12.1 1.0
O A:HOH668 4.2 25.1 1.0
ND1 A:HIS189 4.2 11.5 1.0
O A:HOH517 4.2 28.0 1.0
CG A:HIS189 4.3 10.6 1.0
CB A:ASP191 4.5 10.5 1.0
O A:HOH966 4.6 20.3 0.3
C4 A:AKG404 4.6 19.7 0.7
O A:HOH856 4.7 44.9 1.0
CA A:ASP191 4.9 9.8 1.0
CZ A:PHE283 5.0 17.0 0.5

Manganese binding site 2 out of 2 in 5mof

Go back to Manganese Binding Sites List in 5mof
Manganese binding site 2 out of 2 in the Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Ethylene Forming Enzyme From Pseudomonas Syringae Pv. Phaseolicola - I222 Crystal Form in Complex with Manganese and 2-Oxoglutarate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn402

b:50.0
occ:0.70
 SG A:CYS317 2.4 25.6 0.5
SG A:CYS317 2.7 29.2 0.5
CB A:CYS317 3.4 24.6 0.5
CB A:CYS317 3.4 24.5 0.5
OH A:TYR318 4.3 31.0 1.0
O A:HOH591 4.6 26.8 1.0
OE2 A:GLU84 4.6 40.0 1.0
SD A:MET313 4.6 31.2 1.0
CZ A:TYR318 4.7 27.3 1.0
CG A:MET313 4.7 26.9 1.0
CA A:CYS317 4.8 23.9 0.5
CA A:CYS317 4.8 23.9 0.5
CE2 A:TYR318 4.8 22.4 1.0
CD A:GLU84 4.8 39.3 1.0
CE2 A:TYR192 5.0 13.4 1.0
NH1 A:ARG171 5.0 35.1 1.0

Reference:

Z.Zhang, T.J.Smart, H.Choi, F.Hardy, C.T.Lohans, M.I.Abboud, M.S.W.Richardson, R.S.Paton, M.A.Mcdonough, C.J.Schofield. Structural and Stereoelectronic Insights Into Oxygenase-Catalyzed Formation of Ethylene From 2-Oxoglutarate. Proc. Natl. Acad. Sci. V. 114 4667 2017U.S.A..
ISSN: ESSN 1091-6490
PubMed: 28420789
DOI: 10.1073/PNAS.1617760114
Page generated: Sun Oct 6 01:59:36 2024

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