Manganese in PDB 5lpv: Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana

All present enzymatic activity of Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana:
2.7.10.1; 2.7.11.1;

The structure of Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana, PDB code: 5lpv was solved by D.Bojar, J.Martinez, M.Hothorn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.48 / 2.70
Space group	P 62
Cell size a, b, c (Å), α, β, γ (°)	115.630, 115.630, 49.640, 90.00, 90.00, 120.00
R / Rfree (%)	19.1 / 23.3

The binding sites of Manganese atom in the Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana (pdb code 5lpv). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana, PDB code: 5lpv:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1201 b:86.3 occ:1.00 N3B A:ANP1200 1.8 0.7 1.0 O2A A:ANP1200 1.9 96.5 1.0 OD1 A:ASN1014 2.3 69.7 1.0 OD2 A:ASP1027 2.5 66.2 1.0 PG A:ANP1200 2.9 0.4 1.0 O2G A:ANP1200 3.1 68.4 1.0 PB A:ANP1200 3.1 0.1 1.0 PA A:ANP1200 3.2 94.5 1.0 CG A:ASN1014 3.3 53.1 1.0 O3A A:ANP1200 3.5 0.1 1.0 ND2 A:ASN1014 3.6 52.5 1.0 O3G A:ANP1200 3.6 87.8 1.0 CG A:ASP1027 3.7 70.6 1.0 OG A:SER1013 3.8 63.4 1.0 NZ A:LYS1011 3.9 67.1 1.0 O1B A:ANP1200 3.9 90.4 1.0 O5' A:ANP1200 4.2 66.2 1.0 O1G A:ANP1200 4.2 0.7 1.0 CB A:ASP1027 4.2 52.9 1.0 CE A:LYS1011 4.2 66.1 1.0 O1A A:ANP1200 4.2 64.4 1.0 O2B A:ANP1200 4.3 0.5 1.0 C5' A:ANP1200 4.4 58.8 1.0 O A:SER1013 4.5 77.8 1.0 MN A:MN1202 4.5 0.5 1.0 CB A:ASN1014 4.7 51.5 1.0 OD1 A:ASP1027 4.7 74.0 1.0 O3' A:ANP1200 4.8 74.8 1.0 C A:SER1013 4.8 67.7 1.0 CB A:SER1013 4.9 66.3 1.0 CA A:ASN1014 5.0 56.8 1.0 OD2 A:ASP1009 5.0 72.8 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the BRI1 Kinase Domain (865-1160) in Complex with Amppnp and Mn From Arabidopsis Thaliana within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1202 b:0.5 occ:1.00 O1B A:ANP1200 2.4 90.4 1.0 OD1 A:ASP1027 2.5 74.0 1.0 OD2 A:ASP1027 2.7 66.2 1.0 CG A:ASP1027 2.9 70.6 1.0 O1G A:ANP1200 3.4 0.7 1.0 PB A:ANP1200 3.6 0.1 1.0 N3B A:ANP1200 3.6 0.7 1.0 PG A:ANP1200 3.8 0.4 1.0 O2G A:ANP1200 3.9 68.4 1.0 OE1 A:GLU923 3.9 0.6 1.0 CB A:PHE894 4.0 0.0 1.0 OD2 A:ASP1009 4.1 72.8 1.0 O2B A:ANP1200 4.3 0.5 1.0 CB A:ASP1027 4.4 52.9 1.0 MN A:MN1201 4.5 86.3 1.0 CA A:GLY1029 4.6 55.4 1.0 NZ A:LYS911 4.6 49.1 1.0 N A:GLY1029 4.7 51.2 1.0 O3A A:ANP1200 4.8 0.1 1.0 CG A:MET1030 4.9 82.7 1.0

D.Bojar, J.Martinez, J.Santiago, V.Rybin, R.Bayliss, M.Hothorn. Crystal Structures of the Phosphorylated BRI1 Kinase Domain and Implications For Brassinosteroid Signal Initiation. To Be Published.