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Manganese in PDB 5kg1: Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree

Enzymatic activity of Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree

All present enzymatic activity of Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree:
2.7.7.7;

Protein crystallography data

The structure of Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree, PDB code: 5kg1 was solved by Y.Gao, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.93 / 1.62
Space group P 61
Cell size a, b, c (Å), α, β, γ (°) 98.240, 98.240, 82.010, 90.00, 90.00, 120.00
R / Rfree (%) 18.1 / 22.2

Manganese Binding Sites:

The binding sites of Manganese atom in the Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree (pdb code 5kg1). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree, PDB code: 5kg1:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in 5kg1

Go back to Manganese Binding Sites List in 5kg1
Manganese binding site 1 out of 3 in the Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn501

b:10.8
occ:1.00
 OP1 P:DA9 2.0 10.2 0.2
OD2 A:ASP13 2.1 11.4 1.0
OD1 A:ASP115 2.1 9.5 1.0
OE2 A:GLU116 2.2 11.7 1.0
O3' P:DT8 2.2 11.2 0.2
O A:HOH771 2.2 11.5 1.0
O3' P:DT8 2.3 10.1 0.8
O1A A:DTP506 2.4 13.4 0.8
P P:DA9 2.6 13.0 0.2
CG A:ASP115 3.1 9.7 1.0
CD A:GLU116 3.2 12.0 1.0
CG A:ASP13 3.2 10.0 1.0
C3' P:DT8 3.2 13.4 0.8
OD2 A:ASP115 3.4 10.1 1.0
C3' P:DT8 3.4 14.1 0.2
PA A:DTP506 3.5 9.3 0.8
OD1 A:ASP13 3.6 11.3 1.0
MN A:MN502 3.6 10.5 1.0
OP2 P:DA9 3.7 13.3 0.2
OG A:SER113 3.8 12.6 1.0
OE1 A:GLU116 3.8 14.8 1.0
O2A A:DTP506 3.9 12.2 0.8
O5' P:DA9 3.9 10.8 0.2
O5' A:DTP506 4.0 9.1 0.8
C4' P:DT8 4.1 15.1 0.8
C4' P:DT8 4.1 16.2 0.2
CB A:GLU116 4.1 9.6 1.0
CG A:GLU116 4.1 9.2 1.0
NZ A:LYS224 4.1 7.4 0.8
C5' A:DTP506 4.2 10.7 0.8
C5' P:DA9 4.2 10.6 0.2
O A:HOH738 4.3 13.6 0.7
O A:HOH738 4.3 11.8 0.3
O P:HOH203 4.5 14.9 1.0
CB A:ASP13 4.5 9.1 1.0
C5' P:DT8 4.5 20.3 0.2
CB A:ASP115 4.5 8.5 1.0
C2' P:DT8 4.5 13.0 0.8
C A:ASP115 4.6 11.1 1.0
C5' P:DT8 4.7 20.6 0.8
O5 A:DPO507 4.7 9.8 0.2
O A:ASP115 4.7 10.2 1.0
O1G A:DTP506 4.8 9.7 0.8
O3A A:DTP506 4.8 10.6 0.8
CB A:SER113 4.8 12.3 1.0
C2' P:DT8 4.8 15.7 0.2
N A:GLU116 4.9 8.6 1.0
O A:HOH802 5.0 22.5 1.0
O1B A:DTP506 5.0 8.1 0.8

Manganese binding site 2 out of 3 in 5kg1

Go back to Manganese Binding Sites List in 5kg1
Manganese binding site 2 out of 3 in the Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn502

b:10.5
occ:1.00
 O5 A:DPO507 2.1 9.8 0.2
O1B A:DTP506 2.1 8.1 0.8
OD1 A:ASP13 2.1 11.3 1.0
OD2 A:ASP115 2.1 10.1 1.0
O1G A:DTP506 2.2 9.7 0.8
O1A A:DTP506 2.2 13.4 0.8
O A:MET14 2.3 9.6 1.0
O1 A:DPO507 2.3 8.7 0.2
OP1 P:DA9 2.4 10.2 0.2
CG A:ASP13 3.1 10.0 1.0
PB A:DTP506 3.1 9.7 0.8
CG A:ASP115 3.2 9.7 1.0
P1 A:DPO507 3.4 10.5 0.2
PG A:DTP506 3.4 10.7 0.8
P2 A:DPO507 3.4 10.8 0.2
PA A:DTP506 3.4 9.3 0.8
C A:MET14 3.4 10.4 1.0
O3A A:DTP506 3.4 10.6 0.8
OD2 A:ASP13 3.4 11.4 1.0
MN A:MN501 3.6 10.8 1.0
O3B A:DTP506 3.6 8.7 0.8
O2 A:DPO507 3.6 10.8 0.2
OD1 A:ASP115 3.7 9.5 1.0
NZ A:LYS231 3.7 14.1 1.0
O4 A:DPO507 3.8 9.0 0.2
P P:DA9 3.8 13.0 0.2
N A:MET14 3.9 9.4 1.0
O7 A:DPO507 3.9 10.4 0.2
O A:HOH738 3.9 13.6 0.7
O2G A:DTP506 4.0 10.3 0.8
C5' A:DTP506 4.0 10.7 0.8
CA A:MET14 4.1 10.7 1.0
C5' P:DA9 4.2 10.6 0.2
O5' A:DTP506 4.2 9.1 0.8
O A:HOH738 4.2 11.8 0.3
C A:ASP13 4.2 11.1 1.0
CB A:ASP13 4.4 9.1 1.0
N A:ASP15 4.4 8.6 1.0
O A:HOH771 4.4 11.5 1.0
O5' P:DA9 4.4 10.8 0.2
N A:CYS16 4.5 7.9 1.0
O2B A:DTP506 4.5 10.1 0.8
CB A:ASP115 4.5 8.5 1.0
CE A:LYS231 4.5 18.3 1.0
O6 A:DPO507 4.6 10.4 0.2
CA A:ASP15 4.6 8.0 1.0
O3G A:DTP506 4.6 10.2 0.8
O2A A:DTP506 4.6 12.2 0.8
O A:ASP13 4.6 10.0 1.0
CB A:MET14 4.6 12.8 1.0
O3 A:DPO507 4.7 10.1 0.2
OP2 P:DA9 4.7 13.3 0.2
C A:ASP15 4.7 9.4 1.0
N A:PHE17 4.7 10.3 1.0
O3' P:DT8 4.7 11.2 0.2
CA A:ASP13 4.8 10.9 1.0
CB A:PHE17 4.9 9.5 1.0
O A:ASP115 5.0 10.2 1.0

Manganese binding site 3 out of 3 in 5kg1

Go back to Manganese Binding Sites List in 5kg1
Manganese binding site 3 out of 3 in the Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Human Dna Polymerase Eta-Dna Ternary Complex: Reaction First with 1 Mm MN2+ For 1800S Then with 5 Mm MN2+ For 60S at 30 Degree within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Mn101

b:17.6
occ:0.25
 O2A A:DTP506 1.7 12.2 0.8
OP2 P:DA9 2.1 13.3 0.2
O A:HOH865 2.1 17.9 0.2
O2 A:DPO507 2.2 10.8 0.2
O3A A:DTP506 2.3 10.6 0.8
O A:HOH738 2.4 11.8 0.3
O A:HOH647 2.5 29.6 1.0
PA A:DTP506 2.5 9.3 0.8
NH1 A:ARG61 2.5 21.0 0.8
O A:HOH665 2.8 18.0 1.0
P P:DA9 3.3 13.0 0.2
O1A A:DTP506 3.4 13.4 0.8
CZ A:ARG61 3.6 20.1 0.8
P1 A:DPO507 3.7 10.5 0.2
O5' A:DTP506 3.7 9.1 0.8
OP1 P:DA9 3.7 10.2 0.2
PB A:DTP506 3.7 9.7 0.8
NH2 A:ARG61 3.7 14.9 0.8
O A:HOH738 3.9 13.6 0.7
O5' P:DA9 3.9 10.8 0.2
O6 A:DPO507 3.9 10.4 0.2
O3G A:DTP506 3.9 10.2 0.8
O A:HOH698 4.0 14.7 0.2
O5 A:DPO507 4.0 9.8 0.2
O3B A:DTP506 4.0 8.7 0.8
O A:HOH761 4.0 29.9 0.6
O4 A:DPO507 4.0 9.0 0.2
O A:HOH922 4.1 19.1 1.0
O1G A:DTP506 4.1 9.7 0.8
P2 A:DPO507 4.2 10.8 0.2
O A:HOH771 4.3 11.5 1.0
PG A:DTP506 4.3 10.7 0.8
O2B A:DTP506 4.4 10.1 0.8
O3 A:DPO507 4.5 10.1 0.2
O P:HOH218 4.5 21.9 1.0
O3' P:DT8 4.5 11.2 0.2
C3' P:DT8 4.6 14.1 0.2
C2' P:DT8 4.6 13.0 0.8
O1 A:DPO507 4.6 8.7 0.2
O1B A:DTP506 4.8 8.1 0.8
C3' P:DT8 4.8 13.4 0.8
NE A:ARG61 4.8 20.9 0.8
C8 A:DTP506 4.9 12.8 0.8
C2' P:DT8 4.9 15.7 0.2
C5' A:DTP506 4.9 10.7 0.8
C5' P:DA9 5.0 10.6 0.2
O3' P:DT8 5.0 10.1 0.8
C8 P:DA9 5.0 13.0 0.2
O P:HOH216 5.0 24.8 1.0

Reference:

Y.Gao, W.Yang. Capture of A Third MG2+ Is Essential For Catalyzing Dna Synthesis. Science V. 352 1334 2016.
ISSN: ESSN 1095-9203
PubMed: 27284197
DOI: 10.1126/SCIENCE.AAD9633
Page generated: Tue Dec 15 04:42:59 2020

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