Manganese in PDB 5jht: Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate

The structure of Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate, PDB code: 5jht was solved by S.Fudo, N.Yamamoto, M.Nukaga, T.Odagiri, M.Tashiro, T.Hoshino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.35 / 1.75
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	66.859, 66.859, 128.003, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 23.8

The binding sites of Manganese atom in the Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate (pdb code 5jht). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate, PDB code: 5jht:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn301 b:29.2 occ:1.00 OE2 A:GLU119 2.1 33.5 1.0 O4 A:TLA303 2.1 30.9 1.0 OD2 A:ASP108 2.2 23.1 1.0 O3 A:TLA303 2.3 25.1 1.0 O A:ILE120 2.3 24.7 1.0 NE2 A:HIS41 2.3 26.6 1.0 C4 A:TLA303 3.0 42.7 1.0 CG A:ASP108 3.0 29.6 1.0 C3 A:TLA303 3.1 34.3 1.0 CE1 A:HIS41 3.1 23.5 1.0 CD A:GLU119 3.2 31.0 1.0 OD1 A:ASP108 3.3 24.9 1.0 CD2 A:HIS41 3.4 24.8 1.0 C A:ILE120 3.4 26.9 1.0 MN A:MN302 3.8 34.2 1.0 OE1 A:GLU119 3.8 29.0 1.0 N A:ILE120 3.8 21.6 1.0 O A:HOH423 3.9 30.8 1.0 CA A:ILE120 4.1 19.0 1.0 O41 A:TLA303 4.2 43.8 1.0 CG A:GLU119 4.2 30.9 1.0 O2 A:TLA303 4.2 41.6 1.0 ND1 A:HIS41 4.3 23.2 1.0 NZ A:LYS134 4.3 33.3 1.0 C2 A:TLA303 4.3 39.0 1.0 CB A:ILE120 4.4 24.0 1.0 O A:HOH456 4.4 25.6 1.0 CG A:HIS41 4.4 25.7 1.0 CB A:ASP108 4.4 22.6 1.0 N A:GLY121 4.5 23.1 1.0 CA A:GLY121 4.6 22.9 1.0 OE1 A:GLU80 4.6 34.2 1.0 C A:GLU119 4.7 23.3 1.0 O A:HOH408 4.7 44.5 1.0 CA A:GLU119 4.9 23.1 1.0 CE A:LYS134 5.0 31.4 1.0

Manganese binding site 2 out of 2 in the Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Apo Form of Influenza Strain H1N1 Polymerase Acidic Subunit N-Terminal Region Crystallized with Potassium Sodium Tartrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn302 b:34.2 occ:1.00 O A:HOH423 2.0 30.8 1.0 OE1 A:GLU80 2.1 34.2 1.0 OD1 A:ASP108 2.2 24.9 1.0 O A:HOH454 2.3 26.8 1.0 O A:HOH482 2.3 34.7 1.0 O4 A:TLA303 2.3 30.9 1.0 C4 A:TLA303 3.2 42.7 1.0 CD A:GLU80 3.2 26.0 1.0 CG A:ASP108 3.3 29.6 1.0 O41 A:TLA303 3.4 43.8 1.0 MN A:MN301 3.8 29.2 1.0 OD2 A:ASP108 3.8 23.1 1.0 CE1 A:HIS41 3.9 23.5 1.0 O A:HOH524 4.0 51.7 1.0 OE2 A:GLU80 4.0 38.3 1.0 O A:HOH408 4.1 44.5 1.0 O A:PRO107 4.1 24.5 1.0 O A:LEU106 4.1 32.0 1.0 CG A:GLU80 4.2 28.6 1.0 C A:PRO107 4.4 23.4 1.0 CB A:GLU80 4.4 26.2 1.0 CA A:ASP108 4.4 19.2 1.0 CB A:ASP108 4.4 22.6 1.0 OE1 A:GLU119 4.4 29.0 1.0 OE2 A:GLU119 4.5 33.5 1.0 NE2 A:HIS41 4.5 26.6 1.0 N A:ASP108 4.6 21.2 1.0 O A:HOH462 4.6 58.5 1.0 C3 A:TLA303 4.6 34.3 1.0 CD A:GLU119 4.9 31.0 1.0 CA A:GLU80 4.9 25.1 1.0 ND1 A:HIS41 5.0 23.2 1.0 O3 A:TLA303 5.0 25.1 1.0

S.Fudo, Q.Fei, M.Nukaga, T.Hoshino. Influence of Precipitants on Molecular Arrangements and Space Groups of Protein Crystals Cryst.Growth Des. V. 17 534 2017.
ISSN: ISSN 1528-7483
DOI: 10.1021/ACS.CGD.6B01385