Manganese in PDB 5j6f: Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate

All present enzymatic activity of Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate:
2.5.1.54; 5.4.99.5;

The structure of Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate, PDB code: 5j6f was solved by A.R.Nazmi, M.Othman, E.J.M.Lang, Y.Bai, T.M.Allison, S.Panjkar, V.L.Arcus, E.J.Parker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	82.65 / 2.75
Space group	P 64
Cell size a, b, c (Å), α, β, γ (°)	95.438, 95.438, 167.452, 90.00, 90.00, 120.00
R / Rfree (%)	18.2 / 23.5

The binding sites of Manganese atom in the Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate (pdb code 5j6f). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate, PDB code: 5j6f:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:74.7 occ:1.00 OD2 A:ASP333 1.7 74.1 1.0 OE2 A:GLU322 2.1 74.8 1.0 OE1 A:GLU322 2.3 64.6 1.0 NE2 A:HIS296 2.4 87.6 1.0 CD A:GLU322 2.5 68.6 1.0 SG A:CYS126 2.6 84.8 1.0 CG A:ASP333 2.9 74.0 1.0 CB A:CYS126 3.1 77.9 1.0 CD2 A:HIS296 3.2 85.2 1.0 CE1 A:HIS296 3.4 87.3 1.0 OD1 A:ASP333 3.8 73.7 1.0 CB A:ASP333 3.8 74.8 1.0 CA A:CYS126 3.9 71.1 1.0 CG A:GLU322 4.0 63.6 1.0 NZ A:LYS155 4.2 93.1 1.0 CG A:HIS296 4.4 80.5 1.0 ND1 A:HIS296 4.4 84.0 1.0 CG2 A:THR295 4.7 69.9 1.0 N A:CYS126 4.8 69.8 1.0 CB A:GLU322 4.8 66.1 1.0 C A:CYS126 5.0 67.4 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of DAH7PS-Cm Complex From Geobacillus Sp. with Prephenate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn401 b:0.5 occ:1.00 OD2 B:ASP333 1.8 74.5 1.0 OE2 B:GLU322 2.2 72.5 1.0 SG B:CYS126 2.4 73.0 1.0 NE2 B:HIS296 2.4 79.4 1.0 OE1 B:GLU322 2.5 64.2 1.0 CD B:GLU322 2.6 64.8 1.0 CB B:CYS126 3.0 74.3 1.0 CG B:ASP333 3.0 72.6 1.0 CE1 B:HIS296 3.3 85.1 1.0 CD2 B:HIS296 3.3 78.3 1.0 CA B:CYS126 3.8 69.8 1.0 OD1 B:ASP333 3.9 72.5 1.0 CB B:ASP333 3.9 71.6 1.0 CG B:GLU322 4.1 61.7 1.0 ND1 B:HIS296 4.4 81.2 1.0 CG B:HIS296 4.5 78.5 1.0 C B:CYS126 4.9 68.2 1.0 N B:CYS126 4.9 67.3 1.0

A.R.Nazmi, E.J.M.Lang, Y.Bai, T.M.Allison, M.H.Othman, S.Panjikar, V.L.Arcus, E.J.Parker. Interdomain Conformational Changes Provide Allosteric Regulation En Route to Chorismate. J. Biol. Chem. V. 291 21836 2016.
ISSN: ESSN 1083-351X
PubMed: 27502275
DOI: 10.1074/JBC.M116.741637